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ISSN 1995-5537

Scientific journal

"БІОТЕХНОЛОГІЯ"
V. 4, No. 5, 2011

"Biotechnology" journal V. 5, No. 1, 2012
Р. 9-17, Bibliography 46, Ukrainian.
Universal Decimal classification: 577.112:616

THE ROLE OF TRANSCRIPTION FACTOR ZXDC
IN THE REGULATION OF PROMYELOCYTES DIFFERENTIATION

O. H. Minchenko, O. V. Galkin, D. O. Minchenko

Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv
University of Kansas Medical Center, USA
Bohomoletz National Medical University, Kyiv

In the review the data concerning role of ZDXC transcription factor in the regulation of gene expression, especially genes participated in regulation of cell differentiation, was analyzed. Of the large number of genes whose expression changed significantly when expression of ZDXC transcription factor enhanced in human embryonic kidney cells HEK293 and in human promyelocytes HL60, only some genes (ERG2, PU.1, C/EBP?, C/EBP?, GFI and LRG1) were studied in detail and analyzed. Using EGR2 gene, encoding synthesis of an important transcription factor regulating differentiation, the molecular mechanisms of its regulation under ZDXC transcription factor were considered in detail by binding the EGR2 gene to the promoter. The mechanisms regulating ZDXC gene transcription were analyzed with the following transcription factors dependent on it: overexpression of the key factors of myeloid differentiation (C/EBP?, C/EBP? and PU.1) in HL60 cells led to decresing of expression level of endogenous mRNA of ZXDC transcription factor. Moreover, overexpression of GFI1 factor even blocked completely ZXDC mRNA expression. Data of the review clearly demonstrated the important role of ZDXC transcription factor in regulation of promyelocytes differentiation processes.

Key words: ZDXC transcription factor, trascription regulation, differentiation factors, EGR2, HEK293 cells, HL60 cells.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008