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ISSN 1995-5537

Scientific journal

БІОТЕХНОЛОГІЯ

V. 3, No. 2, 2010

«Biotechnology» journal, Vol. 3, No. 2, 2010
Р. 19-32, Bibliography. 60, Russian..
Universal Decimal classification: 631.811.98 75.117.2.577.2.08

CHANGE OF FUNCTIONALLY ACTIVE CYTOPLASMICAL mRNA POPULATIONS IN PLANT CELLS UNDER GROWTH REGULATORS ACTION AND BIOLOGICAL PERSPECTIVES OF CELL-FREE SYSTEMS OF PROTEIN SYNTHESIS

V. A. Tsygankova, L. I. Musatenko, S. P. Ponomarenko,
L. O. Galkina, Ja. V. Andrusevich, A. P. Galkin

Institute of Bioorganic Chemistry and Petrochemistry
of National Academy of Sciences of Ukraine, Kyiv
Kholodny Institute of Botany of National Academy of Sciences of Ukraine, Kyiv;
State Enterprise «Interdepartmental Research and Technological Center «Agrobiotech»
of National Academy of Sciences and Department of Health of Ukraine, Kyiv

Protein-synthesized activity of poly(А)+mRNA of the embryonic axes of haricot bean germs without and with treatment by growth regulators in the cell-free wheat germ system of protein synthesis in vitro was studied. It is found that mRNA preparations isolated from plants with the stimulated growth both a synthetic regulator (ivin) and regulators of natural origin (emistim, biolan) provide higher level of protein biosynthesis as compared with control. mRNAs obtained from plants with the stimulated growth by natural regulators are characterized by higher potential in stimulation of protein synthesis in vitro, and by more prolonged protein-synthesized activity as compared with synthetic preparation as well. It is assumed that the indicated difference between intensity and duration of protein biosynthesis is related to difference in mRNA populations out of the control plants and of the plants with stimulated with the regulators growth that have been already found.

Key words: the wheat-germ cell-free systems of protein synthesis, plant growth regulators, the proteinsynthesized activity of mRNA in vitro.


© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008