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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

"Biotechnologia Acta" V. 12, No 2, 2019
Р. 63-70, Bibliography 46, English
Universal Decimal Classification:  571.27: 581.6
https://doi.org/10.15407/biotech12.02.063

PHYTOCHEMICAL SCREENING OF POLYHERBAL COMPOSITION BASED ON Portulaca oleracea AND IT’S EFFECT ON MACROPHAGE OXIDATIVE METABOLISM

M. Gahramanova1, 2, R. Dovhyi2, M. Rudyk2, O. Molozhava2, V. Svyatetska2, L. Skivka2

1Nargiz Medical Center, Baku, Azerbaijan
2Education Scientific Center “Institute of Biology and Medicine”,Taras Shevchenko National University of Kyiv, Ukraine

The aim of the work was to explore phytochemical characteristics of water extract from polyherbal composition based on P. oleracea and it’s effect on oxidative metabolism of murine peritoneal macrophages. The qualitative phytochemical analysis was conducted by colorimetric method. Quantitative analysis of phenols was performed in the test with gallic acid as a standard. Murine peritoneal macrophages were isolated without previous sensitization. Leukotoxicity of the water extract from polyherbal composition leukotoxicity was evaluated in MTT test. Reactive oxygen species generation was assayed by the nitroblue tetrazolium reduction method. Phytochemical analysis revealed the presence of water-soluble and insoluble phenols, tannins, saponins, flavonoids, cardiac glycosides and coumarins in the studied plant mixture. The water extract from polyherbal composition used in a range of concentration 1–1000 μg/ml (lyophilisate in distilled H2O) didn’t exhibit any toxic effects on murine peritoneal macrophages. Water extract from polyherbal composition caused statistically significant dose-dependent increase in oxidative metabolism of murine peritoneal suggest modulatory effect of studied water extract from polyherbal composition on innate immunity cells.

Key words: water extract from polyherbal composition Portulaca oleracea, peritoneal macrophages, reactive oxygen species.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2019