Biotechnologia Acta

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"Biotechnologia Acta" V. 11, No 6, 2018
https://doi.org/10.15407/biotech11.06.082
Р. 82-91, Bibliography 37, English
Universal Decimal Classification: 579.663

THE PROPERTIES OF SURFACTANTS SYNTHESIZED BY Acinetobacter calcoaceticus ІMV В-7241 ON REFINED AND WASTE SUNFLOWER OIL

T. P. Pirog, D. A. Lutsai, S. I. Antonuk, I. V. Elperin

Abstract

The aim of the work was to compare the antimicrobial and anti-adhesive activity (including the ability to destroy biofilms), as well as the effect on oil degradation of the surfactants synthesized by the culture of Acinetobacter calcoaceticus IMV B-7241 on refined waste sunflower oil.

The surfactants were extracted from supernatant of cultural liquid by mixture of chloroform and methanol (2:1). The number of attached cells and the degree of biofilm destruction were analyzed spectrophotometrically. Antimicrobial properties of the surfactants were determined by index of the minimal inhibiting concentration (MIC). The concentration of oil in water was analyzed by the gravimetric method after extraction with hexane.

It was shown that surfactants synthesized in medium with 2% of both refined and waste oil were characterized by high antimicrobial (MIC with respect to bacterial test cultures 0.8–29 μg/ml, Candida albicans D-6 26 — 58 μg/ml) and anti-adhesive (decreasing number of bacterial and fungal cells of test cultures attached to abiotic surfaces by 35–70%, destruction of biofilms by an average of 40–44%) activity. Increasing concentration of waste oil in the medium to 4% was accompanied by the formation of surfactants with low antimicrobial activity, in the presence of which the degree of oil destruction in water (3–6 g/l) was 80–88% in 20 days, which is 10–16% higher than when using surfactants synthesized in a medium with 2% oil.

The obtained data indicate on the need for studies on the effect of cultivation conditions of producer on the properties of synthesized surfactants for the production of final product with stable predetermined properties, depending on the field of practical application.

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ПРИРОДНЫЕ И СИНТЕТИЧЕСКИЕ ТВЕРДЫЕ НОСИТЕЛИ В ПРОТОЧНОМ МОДУЛЕ ДЛЯ МИКРОБНОГО ОЧИЩЕНИЯ ФИЛЬТРАТОВ СТОЧНЫХ ВОД Таширев О. Б., Сіома І. Б., Таширева Г. О., Говоруха В. М.

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Biotechnologia Acta, V. 11, No 6, 2018
https://doi.org/10.15407/biotech11.06.073
Р. 73-81, Bibliography 16, English
Universal Decimal Classification: 579.695

NATURAL AND SYNTHETIC SOLID CARRIERS IN FLOW MODULE FOR MICROBIAL SEWAGE FILTRATE PURIFICATION

O. B. Tashyrev, I. B. Sioma, G. O. Tashyreva, V. M. Hovorukha

Abstract

The aim of the research was to develop theoretic principles of an efficient biotechnology for microbial purification of concentrated sewage filtrate from a wide range of organic and non-organic compounds, and experimentally confirmed these principles. The experiment combined conventional microbiological, physical and chemical methods in which ten different types of solid carriers, both natural and artificial, were used. A comparative analysis of these types of solid carriers used in the flow system and a structured evaluation of treatment parameters was provided. The obtaining results demonstrated the effectiveness of the use of approach sewage microbial purification in a flow system. Developed principles can be used as a basis for new efficient biotechnologies for sewage microbial purification.

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Biotechnologia Acta, V. 11, No 6, 2018
https://doi.org/10.15407/biotech11.06.067
Р. 67-72, Bibliography 21, English
Universal Decimal Classification: 616-089.843:612.419-018.4

ABILITY OF THYMIC MSCs AND THEIR DERIVATIVES TO INTERACT WITH THE CELLS OF LYMPHOID ORIGIN

D. L. Demchenko

Abstract

The aim of the research was to determine the ability of thymic multipotent stromal cells and their derivatives to interact with lymphocytes obtained from different sources. It was shown that a part of thymic cells from 6−8-weeks-old С57BL mice in vitro were characterized by such properties: the ability to adhere to the surfaces of cell-culture plastic, the specific fibroblast-like morphology, and the ability to directed adipogenic and osteogenic differentiation. Due to these properties, the cell populations isolated from thymus could be attributed to the multipotent mesenchymal stromal cells (MMSC) or mesenchymal stem cells (MSCs). We have shown that all types of stromal cells have an ability to interact with the lymphoid cells obtained from different sources (thymocytes, splenocytes, cells of the lymph nodes and bone marrow). The largest number of intercellular associations has been formed with the thymocytes, and the smallest one – with the lymphoid cells of bone marrow. Among differentiated forms osteogenic cells are capable to create higher number of intercellular associations, as compared to adipocytes. Thus, probably the intercellular contact interactions between the MSCs and hematopoietic cells might be used as one of the new approaches for efficient and directed modification of the cell properties.

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Biotechnologia Acta, V. 11, No 6, 2018
https://doi.org/10.15407/biotech11.06.055
Р. 55-66, Bibliography 17, English
Universal Decimal Classification: 552.57:579.6

OPTIMIZATION OF THE COAL BACTERIAL DESULFURIZATION USING MATHEMATICAL METHODS

I. A. Blaydа, N. Yu. Vasylieva, T. V. Vasylieva, L. I. Sliusarenko, O. I. Dzhambek

Abstract

The aim of the work was to optimize the process of bacterial desulfurization of energy coal, namely, to determine the influence of the component composition of the nutrient medium and the conditions of the process, which ensure the maximal development and activity of the aboriginal association of acidophilic chemolithotrophic bacteria and, as a consequence, the maximal index of sulfur decrease in coal in minimal time. We used the method of mathematical planning of the experiment adapted to the plan in Greek-Latin squares. The calculations in this approach are based on the analysis of variance (ANOVA). The formal planning of experiments has been carried out with four operating factors (nutrient medium components) at four levels (concentrations). The calculations were performed in Excel. The selection of operating factors and their combinations was made with the usage of unifactor ANOVA, correlation analysis and the method of principal components PCA. Researches were carried out in R 3.4.0 program and were founded on data of the preliminary evaluating experiments. Acidithiobacillus ferrooxidans Coal 17 aboriginal strain was used to obtain the most significant desulfurization effect. This strain was isolated from the investigated coal, studied and identified. The significance of the factor level for each nutrient medium component was analyzed using the Duncan’s multiple range test, the uniformity of the variances was examined with the Cochran’s test, and the significance of the factors was tested by the Fisher’s criterion. As a result, for the optimal nutrient medium the next combination of factors and their levels, which corresponds to the composition, g/dm3, was recommended: (NH4)2SO4 — 0.15; K2HPO4 — 0.50; FeSO4.7H2O — 44.0; KCl — 0.10; MgSO4·7H2O — 0.10; Ca(NO3)2 — 0.10; yeast extract — 0.025% (vol.); strain A. ferrooxidans Coal 17 (titre 1·108 CFU/ml) — 1.60% (vol.). This makes it possible to reduce the sulfur content in coal by 66.31% in a short period (seven days). This result could not be got before.

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Biotechnologia Acta, V. 11, No 6, 2018
https://doi.org/10.15407/biotech11.06.047
Р. 47-54, Bibliography 25, English
Universal Decimal Classification: 57.023: 58.039

NANOSTRUCTURED FERRIC CITRATE EFFECT ON Chlorella vulgarisDEVELOPMENT

N. B. Golub, M. Tsvetkovych, I. I. Levtun, V. I. Maksyn

Abstract

The aim of the research was to study the development of Chlorella vulgaris at culturing on the modified Gromov 6 medium with high concentrations of nanostructured ferric citrate and also its effect on photosynthesis pigments accumulation. It was demonstrated that the highest intracellular iron content (15 mg/g of dry mass) in the culture cells was typical with nanostructured ferric citrate content of 30 mg/dm3 of culture medium, the highest content of chlorophyll a — 23 mg/g of dry algae mass, b — 7.5 mg/g of dry mass, and carotenoids — 9.2 mg/g of dry mass was observed at nanostructured ferric citrate content of 20 mg/dm3. The use of nanostructured ferric citrate leaded to an increase in the chlorella biomass yield by 3 times with compared to standard technology. Simultaneously, intracellular iron content in cells increased significantly with the use of nanostructured ferric citrate, which increases their value as a nutritional supplement. In order to increase the biomass yield and intracellular iron content in cells, application of nanostructured ferric citrate is recommended.

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