Biotechnologia Acta

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Ж-л "Biotechnologia Acta" Т. 11, № 2, 2018
https://doi.org/10.15407/biotech11.03.005

INFORMATION COMPUTER TECHNOLOGIES FOR USING IN BIOTECHNOLOGY: ELECTRONIC MEDICAL INFORMATION SYSTEMS O. M. Klyuchko

Abstract

The aim of the work was to generalize and analyze the use of electronic information systems in biotechnology in order to create new versions of these systems. The publications concerning the systems of different types for solving the problems in biotechnology were studied. Similar systems which traditionally belong to biology and medicine were classified. The prospects of their application for development of more advanced electronic systems were considered.

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"Biotechnologia Acta" V. 11, No 3, 2018
https://doi.org/10.15407/biotech11.03.027

BIOLOGICAL PROPERTIES AND MEDICAL APPLICATION OF DIPHTHERIA TOXIN DERIVATIVES Manoilov K.Y.

Abstract

The aim of the review was to analyze the literature data related to the application of a variety of diphtheria toxin derivatives. Although studies of the diphtheria toxin interaction with sensitive and resistant mammalian cells have been held for a relatively long time, there are still some unresolved issues concerning the molecular mechanisms of diphtheria toxin functioning. Native diphtheria toxin  and parts of its molecule which preserves toxicity are used as instruments in the newest biotechnological methods of specific cell subtype ablation in multicellular organisms. New recombinant derivatives of diphtheria toxin are periodically obtained in the laboratories throughout the world. Most of these analogs of diphtheria toxin  are used in biological studies as convenient tools for analysis of the functions of the natural toxin. A non-toxic analog of diphtheria toxin, protein CRM197, is used in clinical practice as a component of vaccines and as an anticancer agent. Diphtheria toxin  -based targeted toxin therapy is another perspective trend for cancer therapy. Therefore, studying of diphtheria toxin derivatives is of a great relevance for biotechnology and medicine.

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"Biotechnologia Acta" V. 11, No 3, 2018
https://doi.org/10.15407/biotech11.03.047

APPLICATION OF BIOFILMS IN REMOVAL OF HEAVY METALS FROM WASTEWATER IN STATIC CONDITION Ogbuagu Dike Henry, Nwachukwu Ikenna Ndubuisi, Ejike Onyinye Joy

Abstract

The aim of the research was to utilize biofilms as a model in ecotoxicology to remove selected heavy metals (Cd, Cu, Cr, Zn and Pb) from wastewater in a static condition. Biofilms were grown in three graded concentrations of the metal leachates (0.625, 0.417 and 0.250 %), harvested after 1, 2 and 3 weeks and analyzed for heavy metals. Mean accumulations peaked on Day 21, and of Cd ranged from 0.000 to 0.040 (mean = 0.00837 ± 0.002), Cu from 0.000 to 0.212 (meam = 0.03929 ± 0.012), Cr from 0.000 to 0.500 (mean = 0.05821 ± 0.021), Zn from 0.000 to 1.456 (mean = 0.31833 ± 0.109) and Pb from 0.000 to 0.099 (mean = 0.02129 ± 0.006) mg/g in resultant biofilm formations. Accumulation of the metals increased significantly with time [F(205.59) > Fcrit(3.95)] at the 95% confidence interval. Those of Pb was significantly higher in the 0.625% leachate mixture than control (Sig F = 0.034) at P < 0.05, even as those of Cd and Cu were slightly higher in the concentrations than control. Biofilm model removed small amounts of metals from wastewater stream in static condition.

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"Biotechnologia Acta" V. 11, No 3, 2018
https://doi.org/10.15407/biotech11.03.056

BIOSYNTHESIS OF VOLATILES BY Pleurotus ostreatus (Jacq.:Fr.) Kumm. MUSHROOMS ON SUBSTRATES ENRICHED WITH VEGETABLE OILS Vlasenko E. N., Kuznetsova O. V.

The purpose of the study was to analyze possible ways and intensity of synthesis of volatile flavor compounds by Pleurotus ostreatus (Jacq.:Fr.) Kumm. mushrooms in the process of intensive cultivation on sunflower husk and barley straw with the addition of vegetable oils (sunflower and corn) as a potential source of unsaturated fatty acids. Methods of sensory profile analysis and ultraviolet spectroscopy were used. Sensory profile analysis of dried samples of fruit bodies showed an increase in the intensity of mushroom, meat and grassy notes of flavor on substrates with the addition of vegetable oils in a concentration of 1% and 5% of the weight of the substrate. For the strain IBK-551 marked increase in the intensity of sweet and floral attributes of the aroma on both substrates with the addition of corn oil. UV spectroscopy of hexane extracts of dried samples of fruit bodies revealed maxima of light absorption in the range of 200−210 nm and 260−300 nm. There was a difference in intensity of light absorption of samples of different strains cultivated on substrates with the addition of vegetable oils.

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"Biotechnologia Acta" V. 11, No 3, 2018
https://doi.org/10.15407/biotech11.03.069

GANODERMA SPECIES EXTRACTS: ANTIOXIDANT ACTIVITY AND CHROMATOGRAPHY V. Raks, M. Öztürk, O. Vasylchenko, M. Raks

Abstract

Research aimed to isolate biologically active compounds from mushrooms fruiting bodies of Ganoderma lucidum, Ganoderma adspersum and Ganoderma applanatum and estimate their antioxidant activities. Variety of sample preparation techniques were used to isolate biologically active compounds: solid-liquid, ultrasonic extraction, Soxhlet extractor extractions. Antioxidant properties were estimated with spectrophotometricaly measuring free radical scavenging activity. High performance liquid chromatography was applied to get fingerprints of isolated extracts. In β-carotene-linoleic acid assay, methanol extracts demonstrated mainly the best antioxidant activities: optimum values of half maximal inhibitory concentration (IC50) − for G. applanatum and G. adspersum were 8.25 ± 0.88 µg/ml and 1.70 ± 1.13 µg/ml respectively. However, petroleum ether and chloroform extracts of G. lucidum demonstrated the highler antioxidant activity with an IC50 about 33.66 ± 3.69 µg/ml. Chromatograms of dry components of acetone and methanol extracts of G. lucidum were recorded. The main outcome of such chromatograms is the possibility to compare presence active components in various mushrooms species without usage of expensive standards.

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