Biotechnologia Acta

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MICROBIAL SYNTHESIS OF PHYTOHORMONES T. P. Pirog , G. O. Iutynska, N. O. Leonova, K. A. Beregova, T. A. Shevchuk

"Biotechnologia Acta" V. 11, No. 1, 2018
https://doi.org/10.15407/biotech11.01.005

AbstractThe aim of the review was to   analyze    current literature data and the results of own studies on the synthesis of auxins, cytokinins, and gibberellins by plant-associated microorganisms (living in rhizosphere, endophytic, nitrogen-fixing, and phytopathogenic), and by those not involved in symbiotic interactions. Many microorganisms can generate phytohormones, and microbial synthesis of indole-3-acetic acid can be enhanced which can be used in producing it instead of extracting it from plants or by chemical synthesis. Recent progress in intensifying the synthesis of gibberellic acid in deep and solid-phase producer cultivation allows substantially reducing the prime cost of biotechnological production of that phytohormone. The ability of microorganisms to simultaneously synthesize phytohormones and other biologically active compounds with antimicrobial, nematocidal, and other various effects enables creating complex polyfunctional microbial preparations with various biological properties for use in crop production to stimulate plant growth and pest control.

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SPECIFICITY  OF MANIFACTURING PROCESS VALIDATION FOR DIAGNOSTIC SEROLOGICAL DEVICES O. Yu. Galkin, A. G. Komar, M. O. Pys’menna

"Biotechnologia Acta" V. 11, No. 1, 2018
https://doi.org/10.15407/biotech11.01.025

Abstract
The aim of this research was to analyze recent scientific literature, as well as national and international legislature on manifacturing process validation of biopharmaceutical production, in particular devices for serological diagnostics. Technology validation in the field of medical devices for serological diagnostics is most influenced by the Technical Regulation for Medical Devices for in vitro Diagnostics State Standards of Ukraine – SSU EN ISO 13485:2015 “Medical devices. Quality management system. Requirements for regulation”, SSU EN ISO 14971:2015 “Medical devices. Instructions for risk management”, Instruction ST-N of the Ministry of Healthcare of Ukraine 42-4.0:2014 “Medications. Suitable industrial practice”, State Pharmacopoeia of Ukraine and Instruction ICH Q9 on risk management. Current recommendations for validations of drugs manufacturing process, including biotechnological manufacturing, can not be directly applied to medical devices for in vitro diagnostics. It was shown that the specifics of application and raw materials require individual validation parameters and process validations for serological diagnostics devices. Critical parameters to consider in validation plans were provided for every typical stage of production of in vitro diagnostics devices on the example of immunoassay kits, such as obtaining protein antigens, including recombinant ones, preparations of mono- and polyclonal antibodies, immunoenzyme conjugates and immunosorbents, chemical reagents etc. The bottlenecks of technologies for in vitro diagnostics devices were analyzed from the bioethical and biosafety points of view.

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SOME TRENDS IN MATHEMATICAL MODELING FOR BIOTECHNOLOGY O. M. Klyuchko, YU. M. Onopchuk

"Biotechnologia Acta" V. 11, No. 1, 2018
https://doi.org/10.15407/biotech11.01.039

Abstract
The purpose of present research  is to demonstrate some trends of development of modeling methods for biotechnology according to contemporary achievements in science and technique. At the beginning the general approaches are outlined, some types of classifications of modeling methods are observed. The role of mathematic methods modeling for biotechnology in present époque of information computer technologies intensive development is studied and appropriate scheme of interrelation of all these spheres is proposed. Further case studies are suggested: some mathematic models in three different spaces (1D, 2D, 3D models) are described for processes in living objects of different levels of hierarchic organization. In course of this the main attention was paid to some processes modeling in neurons as well as in their aggregates of different forms, including glioma cell masses (1D, 2D, 3D brain processes models). Starting from the models that have only theoretical importance for today, we describe at the end a model which application may be important for the practice. The work was done after the analysis of approximately 250 current publications in fields of biotechnology, including the authors’ original works.

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POLYMERASE CHAIN REACTION FOR IDENTIFICATION OF CYPRINID HERPESVIRUSES IN UKRAINE Yu. P. Rud, M. I. Maistrenko, L. P. Buchatskiy

"Biotechnologia Acta" V. 11, No. 1, 2018
https://doi.org/10.15407/biotech11.01.058

Abstract
The aim of the research was to investigate diseased fish species Labeo (Labeo bicolor) and Sack-gill Catfish (Heteropneustes fossilis) by testing several diagnostic systems targeting fish herpesviruses in purpose to determine the etiology of the outbreak infection in aquarium fishes at the Kуiv Zoo during summer 2017. During a fish health inspection of aquariums in Kyiv Zoo, an Cyprinid herpesvirus 3 − CyHV-3 was detected in Labeo and Sack-gill Catfish. Preliminary examination of infected fish revealed a range of lesions particularly in internal organs and tissues. The gills of diseased fish were characterized by hyperaemia and necrosis. The infringement of liver color and structure, kidney swelling and gallbladder necrosis were observed in both fish species. The virus did not grow in fish cell lines of RTG-2, FHM and EPC, that was evident by absence of any morphological changes in appropriate cell lines. Also initially fish were tested for parasitic and bacterial infections and they were determined to be non-infected. Our results demonstrated that specific oligonucleotide primers for thymidine kinase gene of CyHV-3 were successfully amplified the specific DNA fragments. The length of polymerase chain reaction product, as expected, was 264 nucleotide pairs. The amplified specific fragments were identical to the area of thymidine kinase gene CyHV-3, as was shown by sequence analysis. The identity of nucleotide sequences composed 97‒99%. The same positive results were obtained using primers that recommended by the International Epizootic Bureau, fragments in size of 409 and 292 were also obtained. In our opinion, CyHV-3 was brought to Ukraine by import of aquarium fish avoiding sanitary control of transboundary transportation. Therefore, the uncontrolled import of aquarium and cultured fish species is a serious problem because imported fish could be as a source of highly pathogenic infections for industrial aquaculture.

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USING OF LOWLAND PEAT SUBSTRATE FOR CULTIVATION OF PICHIA ANOMALA IMB Y-5067 AND RHODOTORULA GRACILIS IMB Y-5075 YEAST STRAINST. S. Kalandyrets, V. O. Krasinko, H. S. Andriiash, N. Ye. Beiko, О. О. Tigunova, S. M. Shulga

"Biotechnologia Acta" V. 11, No. 1, 2018
https://doi.org/10.15407/biotech11.01.064

Abstract
The aim of the research was to study the accumulation of yeast lipids in the process of cultivation of strains P. anomala IMB Y-5067 and R. gracilis IMB Y-5075 with the use of peat as growing substrate. The object of researches was strains P. anomala IMB Y-5067 and R. gracilis IMB Y-5075 from “Collection of strains of microorganisms and plant lines for food and agricultural biotechnology” of SE “Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine”. As a raw material, lowland peat which was preliminarily processed with the help of cavitation or explosive autohydrolysis was used. The accumulation of lipids over the course of cultivation P. anomala IMB Y-5067 and R. gracilis IMB Y-5075 on non-food substrate ‒ lowland peat was shown. The effect of pulp explosive autohydrolysis and pulp cavitation processing on biomass accumulation and lipids synthesis by strains P. аnomala IMB Y-5067 and R. gracilis IMB Y-5075 was researched. It was found that the maximum lipids accumulation by strains P. аnomala IMB Y-5067 (9.7 g/dm3) and R. gracilis IMB Y-5075 (8.9 g/dm3) was over the course of cavitation processing of peat pulp and additional application of salts and yeast extract into cultivation environment.

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