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"Biotechnologia Acta" V. 11, No 5, 2018
https://doi.org/10.15407/biotech11.05.005

ELECTRONIC INFORMATION SYSTEMS FOR MONITORING OF POPULATIONS AND MIGRATIONS OF INSECTS

O. M. Klyuchko, Z. F. Klyuchko

Abstract

The purpose of the work was to analyze existing information systems (IS) of biological objects and to propose the methods for development of such IS for insects on the example of Noctuidae (Lepidoptera). A detailed analysis of technical information concerning the distributed networked systems, access to computer systems to the common data in electronic IS and the organization of biomedical databases in the Internet was done. The peculiarities of IS’ prototypes development for environmental monitoring of the fauna have been discussed, in particular changes in the number of butterflies’ populations throughout France (including western and southern departments), the Noctuidae (Lepidoptera) in the steppe zone of Ukraine (Striltzivskyi Steppe), and the development of such IS for all territory of Ukraine.

The results could be used to develop electronic IS for other biological organisms.

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"Biotechnologia Acta" V. 11, No 5, 2018
https://doi.org/10.15407/biotech11.05.026

CALIX[4]ARENES METHYLENE BISPHOSPHONIC ACIDS EFFECT ON PLASMIN ACTIVITY

O. I. Yusova, O. V. Savchuk, T. Yatsenko, V. Popadiuk, L. G. Kapustianenko, T. V. Grinenko

Abstract

The aim of the work was to study plasmin inhibiting properties of calix[4]arenes functionalized by phosphonic acid remnants. The following methods were used: turbidimetry, analysis of enzyme activity for chromogenic substrates, evaluation of caseinolytic activity for the release of tyrosine from casein, Dixon’s method for determining the type and inhibition constant. It was found, that calix[4]arenes С 296, С-425, С-427 and C-145 inhibit fibrin clot lysis by plasmin in dose-dependent manner and inhibition rate is proportional to the number of phosphonic acid remnants. C-145 is a most effective plasmin inhibitor (competitive mechanism, Ki = 0,52 μM). However, C-145, as well as С-296, С-425 and С-427, does not affect amidolytic and caseinolytic plasmin activity but inhibit plasminogen activation by streptokinase. Thus, we assume that the mechanism of calyx[4]arene selectivity to fibrinolysis and its ability to obstruct plasminogen-streptokinase interaction is provided by complex formation between calix[4]arene negatively charged phosphonic groups and positively charged amino acids in substrate recognition exosites of plasmin. Calix[4]arene C-145 is effective plasmin fibrinolytic activity inhibitor and perspective for further investigation as antifibrinolytic agent.

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"Biotechnologia Acta" V. 11, No 5, 2018
https://doi.org/10.15407/biotech

DEGRADATION OF FLAVONOIDS BY Cryptococcus albidus α-L-RHAMNOSIDASE

N.V. Borzova O. V., Gudzenko, L. D. Varbanets

Abstract

The aim of the work was to investigate the possibility of practical use substrate specificity of α-Lrhamnosidase Cryptococcus albidus. p-Nitrophenyl derivatives of monosaccharides were used to determine the activity and specificity of the enzyme. The ability to hydrolyze of natural substrates was evaluated by Davis and high-performance liquid chromatography methods. It was shown that the enzyme exhibits narrow specificity towards the glycon of synthetic substrates and hydrolyzes only p-nitrophenyl-α-L-rhamnopyranoside (Km 4.5 mM) and p-nitrophenyl-α-D-glucopyranoside (Km 10.0 mM). C. albidus α-L-rhamnosidase  the most active degrades naringin (Km 0.77 mM), releasing prunin and naringenin. Km for neohesperidin was 3.3 mM. The efficacy of the naringin hydrolysis in grapefruit and pomelo juice was 98 and 94% in 60 min (40 оC, 2 U/ml). As the result of treatment by α -L-rhamnosidase of green tea and orange juice, there was a decrease in the content of rutin, narirutin and hesperidin, indicating that the α -1,2- and α -1,6-linked rhamnose can be cleaved from natural flavonoids. Thus, the study shows the efficiency of treating citrus juices and green tea with C. albidus α -L-rhamnosidase for the purpose of improving their taste qualities and obtaining bioavailable flavonoids glucosides.

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"Biotechnologia Acta" V. 11, No 5, 2018
https://doi.org/10.15407/biotech11.05.042

AMPHIBIAN SKIN SECRETIONS: A POTENTIAL SOURCE OF PROTEOLYTIC ENZYMES

I. Nikolaieva, Yu. Dudkina, D. Oliinyk, O. Oskyrko, O. Marushchak, T. Halenova, O. Savchuk

Abstract

The aim of the work was to study the protein content and proteolytic activity of the skin glands secretions of 10 the most common types of amphibians on the territory of Ukraine such as B. bombina, B. variegata, B. bufo, B. viridis, R. temporaria, P. ridibundus, P. esculentus, P. fuscus, S. salamandra, as well as the hybrid of B. bombina and B. variegata species. It was shown that the skin secretions of the studied amphibians contained a wide range of proteins with a molecular weight in the range from 8 to 150 kDa. By enzyme electrophoresis using gelatin, fibrinogen and collagen as substrates, it was found that they contained proteinases that differ in substrate specificity. It was revealed that the skin glands secretions of B. bombina, S. salamander species, as well as the hybrid of B. bombina and B. variegata species were characterized by he increased protein content with gelatinase and collagen activity.

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"Biotechnologia Acta" V. 11, No 5, 2018
https://doi.org/10.15407/biotech11.05.049

ISOLATION OF PURE CULTURES IRON- AND MANGANESE-OXIDIZING BACTERIA FROM RAPID FILTERS

O. V. Kravchenko, O. S. Panchenko

Abstract

The aim of the research was the isolation from drinking water the pure cultures of iron- and manganese-oxidizing microorganisms with further assessment of their efficacy to remove these contaminants on rapid filters. To assess the effectiveness selected strains were grown on the solid nutrient medium; the suspension was prepared and was treated to zeolite loading. Ten pure cultures of iron- and manganese-oxidizing bacteria were isolated and identified as 6 genuses: Siderocapsa, Leptothrix, Sphaerotillus, Galionella, Metallogenium, Hyphomicrobium. Comparison the efficiency of genuses Leptothrix, Sphaerotillus, Metallogenium has shown that under conditions of these experiments Leptothrix more effectively removed iron and manganese at low concentrations in model solution.

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Dear colleagues, we invite you to publish your articles in our journal.
© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2008.
All rights are reserved. Complete or partial reprint of the journal is possible only with the written permission of the publisher.
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