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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
Biotechnologia Acta V. 13, No 3, 2020
Р. 81-88, Bibliography 15, English
Universal Decimal Classification: 628.3
https://doi.org/10.15407/biotech13.03.081
EFFECTIVE TECHNOLOGY OF PHARMACEUTICAL ENTERPRISES WASTEWATER LOCAL TREATMENT FROM ANTIBIOTICS
National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”
The work was aimed to study the processes of industrial wastewater purification of a pharmaceutical company treatment and to recommend an effective technology for local treatment of such wastewater in accordance with the requirements of normative documents on the treated wastewater disposal into the urban sewerage system.
Model solutions of cefuroxime, a cephalosporin antibiotic, in distilled water with a concentration of 25 and 35 mg/dm3 were used for the study. COD model solutions, which is, respectively, 90 and 120 mg/dm3. Chemical oxygen demand was etermined by the dichromate method (according to the guiding normative document (GND) 211.1.4.021-95. “Methods for Chemical Oxygen Demand (COD) Determining in Surface and Wastewater”).
The proposed technology included the following successive processes of physical and chemical treatment of wastewater: aeration – equalization of quantitative and qualitative composition of wastewater and oxidation up to 30% of organic matter; coagulation – removing of antibiotics from wastewater by their adsorption on a highly developed surface of mineral coagulants flakes; sedimentation – separating of the formed flakes; oxidation of antibiotics destruction products by hydrogen peroxide; filtration – removal of fine impurities.Effect of COD reduction rate in the coagulation and setteling of wastewater in the case of ferrous sulphate III was 79.2% and 75%, which is higher by 4.2-6.7% than when using aluminum sulphate. The COD index changing of the pharmaceutical enterprise wastewater by the stages of its purification was found as follows: “aeration — coagulation with iron sulfate III — sedimentation — oxidation — filtration”. After filtering effect of reducing COD index was 95.8-100% at the initial value of 120 and 90 mg/dm3 respectively. The technology of local wastewater treatment from antibiotics was developed, which was based on the consistent use of physico-chemical methods of treatment and enabled to remove antibiotics and related substances from wastewater in accordance with the requirements of normative documents and disposal of treated wastewater into the urban wastewater system.
Application of the developed technology of local wastewater treatment from antibiotics and related substances by the pharmaceutical companies would lead to meet the requirements of industrial wastewater disposal into the city drainage system, to a significant risk reduction of the antibiotics influence on the microorganisms of active sludge of the urban wastewater biological treatment facilities, to operating costs reduction to achieve maximum allowable discharges (MAD) of wastewater into a natural reservoir.
Key words: antibiotics, wastewater, treatment, pharmaceutical enterprises.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2020
References
1. Angeles L. F., Mullen R. A., Huang I. J., Wilson C., Khunjar W., Sirotkin H. I., McElroy A. E., Aga D. S. Assessing pharmaceutical removal and reduction in toxicity provided by advanced wastewater treatment systems. Environmental Science: Water Research and Technology. 2020, 6 (1), 62–77. https://doi.org/10.1039/C9EW00559E
2. Kurt A., Mert B. K., ?zengin N., Sivrio?lu ?., Yonar T. Treatment of Antibiotics in Wastewater Using Advanced Oxidation Processes (AOPs). Physico-Chemical Wastewater Treatment and Resource Recovery. 2017. https://doi.org/10.5772/67538
3. Collivignarelli M. C., Pedrazzani R., Sorlini S., Abb? A., Bertanza G. H2O2 based oxidation processes for the treatment of real high strength aqueous wastes. Sustainability (Switzerland). 2017, 9 (2), 1–14. https://doi.org/10.3390/su9020244
4. Ghaly M. Y., H?rtel G., Mayer R., Haseneder R. Photochemical oxidation of p-chlorophenol by UV/H2O2 and photo-Fenton process. A comparative study. Waste Management. 2001, 21 (1), 41–47. https://doi.org/10.1016/S0956-053X(00)00070-2
5. Ribeiro A. R., Sures B., Schmidt T. C. Cephalosporin antibiotics in the aquatic environment: A critical review of occurrence, fate, ecotoxicity and removal technologies. Elsevier Ltd. 2018. https://doi.org/10.1016/j.envpol.2018.06.040
6. Ahmed M. B., Zhou J. L., Ngo H. H., Guo W. Adsorptive removal of antibiotics from water and wastewater : Progress and challenges Science of the Total Environment Adsorptive removal of antibiotics from water and wastewater : Progress and challenges. Science of the Total Environment. 2015, 532 (November), 112–126. https://doi.org/10.1016/j.scitotenv.2015.05.130
7. Choi K. J., Kim S. G., Kim S. H. Removal of antibiotics by coagulation and granular activated carbon filtration. Journal of Hazardous Materials. 2008, 151 (1), 38–43. https://doi.org/10.1016/j.jhazmat.2007.05.059
8. Choi K. J., Kim S. G., Kim S. H. Removal of antibiotics by coagulation and granular activated carbon filtration. Journal of Hazardous Materials. 2008, 151 (1), 38–43. https://doi.org/10.1016/j.jhazmat.2007.05.059
9. Carabineiro S. A. C., Thavorn-Amornsri T., Pereira M. F. R., Figueiredo J. L. Adsorption of ciprofloxacin on surface-modified carbon materials. Water Research. 2011, 45 (15), 4583–4591. https://doi.org/10.1016/j.watres.2011.06.008
10. Adams C., Wang Y., Loftin K., Meyer M. Removal of antibiotics from surface and distilled water in conventional water treatment processes. Journal of Environmental Engineering. 2002, 128 (3), 253–260. https://doi.org/10.1061/(ASCE)0733-9372(2002)128:3(253)
11. Elbalkiny H. T., Yehia A. M., Riad S. M., Elsaharty Y. S. Removal and tracing of cephalosporins in industrial wastewater by SPE-HPLC: optimization of adsorption kinetics on mesoporous silica nanoparticles. Journal of Analytical Science and Technology. 2019, 10 (1). https://doi.org/10.1186/s40543-019-0180-6
12. Duan H. Study on the Treatment Process of Wastewater from Cephalosporin Production. Journal of Sustainable Development. 2009, 2 (2), 133–136. https://doi.org/10.5539/jsd.v2n2p133
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14. Suslova O. Resistance of karst caves microorganisms to p-nitrochlorobenzene. Biotechnol. acta. 2015, 8 (4), 135–140. https://doi.org/10.15407/biotech8.04.135
15. Tashyrev O. Natural and synthetic solid carriers in flow module for microbial sewage filtrate purification. Biotechnol. acta. 2018, 11 (6), 73–81. https://doi.org/10.15407/biotech11.06.073
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- Hits: 344
ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
Biotechnologia Acta V. 13, No 3, 2020
Р. 73-80, Bibliography 17, English
Universal Decimal Classification: 579.861.2+57.017.22
https://doi.org/10.15407/biotech13.03.073
In vitro ACTIVITY OF THE ANTIBIOTIC BATUMIN AGAINST Candida albicans BIOFILM
L. N. Churkina 1, N. B. Perunova 2, О. V. Bukharin 2, Е. V. Ivanova 2, L. V. Yaroshenko 1
1 Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, Kyiv
2 Institute of Cellular and Intracellular Symbiosis, Ural Branch of Russian Academy of Sciences, Orenburg, Russian Federation
The aim of this work was to study action of batumin on the strains of Candida albicans and Candida krusei in planktonic and biofilm form and also to obtain more detailed insights into the influence of batumin on biofilm formation by using atomic-force microscopy. The Minimum Inhibitory Concentration (MIC) of batumin was studied according to CLSI standards. Formation of a biofilm was studied by the photometric O'Toole method by means of a plate photometer ELx808 (BioTek, USA) at wavelength of 630 nanometers.
The batumin has a high selective activity against staphylococci (MIC ≥ 0,25 μg/ml), at the same time, antibiotic, being not active concerning yeast of the genus Candida (MIC ≥ 512 μg/ml) showed the inhibiting action on biofilm formation of these microorganisms. Batumin influence on biofilm formation was studied in type, collection strains С. albicans, C. kruise and clinical isolates. Presence 0,125 µg/ml of batumin in the broth (1/2 MIC for staphylococci) reduced the biofilm formation at 55.6% of the studied strains. Their biofilm formation values varied for C. albicans from 1.5?3.9 CU (conventional unit: OD630 in experimental samples / OD630 in control samples), for C. krusei of 2.3?3.0. Batumin was more effective against Candida strains with strong biofilm formation.
Atomic force microscopy revealed qualitative changes in the exopolymeric matrix due to batumin treatment, as well as a significant reduction in the number of cells adhered to the coverslip, preventing formation of C. albicans 127 biofilm, However, C. albicans ATCC 24433 a significant reduction in the number of cells adhered to the coverslip weren't observed.
The data obtained by an Аtomic force microscopy confirm ability of a batumin to prevent formation of a biofilm at the studied strains that allows to consider it as the preventive agent at treatment of the infections caused by yeast-like fungi of the genus Candida.
Key words: batumin, Candida, biofilm, atomic force microscopy.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2020
References
1. Sardi J. C. O., Scorzoni L., Bernardi T., Fusco-Almeida A. M., Mendes Giannini M. J. S. Candida species: current epidemiology, pathogenicity, bio?lm formation, natural antifungal products and new therapeutic options. J. Med. Microbiol. 2013, V. 62, P. 10–24. https://doi.org/10.1099/jmm.0.045054-0
2. Fanning S., Mitchell A. P. Fungal bio?lms. PLoS Pathog. 2012, V. 8, P. e1002585. https://doi.org/10.1371/journal.ppat.1002585
3. Ku T. S. N., Palanisamy S. K., Lee S. A. Susceptibility of Candida albicans bio?lms to azithromycin, tigecycline and vancomycin and the interaction between tigecycline and antifungals. Int. J. Antimicrob. Agents. 2010, V. 36, P. 441–446. https://doi.org/10.1016/j.ijantimicag.2010.06.034
4. Vogel M., K?berlel M., Sc?f?er H., Treiber M., Autenrieth I. B., Schumacher U. K. Rifampicin induced virulence determinants increase. Candida albicans bio?lm formation. F1000Research. 2013, V. 2, P. 106. https://doi.org/10.12688/f1000research.2-106.v1
5. Kiprianova E. A., Klochko V. V., Zelena L. B., Churkina L. N., Avdeeva L. V. Pseudomonas batumici sp.nov., the antibiotic-producing bacteria isolated from soil of the Caucasus Black Sea coast. Microbiol. J. 2011, V. 73, P. 3?8 (In Ukrainian).
6. Klochko V. V., Kiprianova E. A., Churkina L. N., Avdeeva L. V. Antimicrobial spectrum of antibiotic batumin. Microbiol. J. 2008, V. 70, P. 41?46 (In Ukrainian).
7. Bukharin O. V., Churkina L. N., Perunova N. B., Ivanova E. V., Novikova I. V., Avdeeva L. V., Yaroshenko L. V. Influence of antistaphylococcal antibiotic batumin on microorganisms biofilm formation. Zh. Mikrobiol. Epidemiol. Immunobiol. 2012, V. 2, P. 8?12. (In Russian).
8. Performance Standards for Antimicrobial Susceptibility Testing; Fifteenths Informational Supplement. CLSI/ NCCLS Document M100-S. Clinical and Laboratory Standards NCCLS. 2005, 165 p.
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11. Eaton P., Fernandes J. C., Pereira E., Pintado M. E., Xavier Malcata F. Atomic force microscopy study of the antibacterial effects of chitosans on Escherichia coli and Staphylococcus aureus. Ultramicroscopy. 2008, V. 108, P. 1128?1134.https://doi.org/10.1016/j.ultramic.2008.04.015
12. Lakin G. F. Biometry. Higher school. 1990, P. 352.
13. Ivanova Y. V., Churkina L. N., Avdeeva L. V., Perunova N. B. Effect of anti-staphylococcal antibiotic batumin on stages of biofilm formation in Candida albicans. Abstract of the materials of the 3rd Congress of Mycologists of Russia. Modern Micology in Russia, Moscow, Russia. 10?12 October 2013.
14. Churkina L., Vaneechoutte M., Kiprianova E., Perunova N., Avdeeva L., Bukharin O. Batumin ? a selective inhibitor of staphylococci ? reduces biofilm formation in methicillin resistant Staphylococcus aureus. Open J. Med. Microbiol. 2015, N 5, P. 193?201. https://doi.org/10.4236/ojmm.2015.54024
15. Bukharin O. V., Churkina L. N., Perunova N. B., Ivanova Y. V., Vasilchenko A. S., Avdeeva L. V., Yaroshekno L. V. Morpho-functional changes in biofilms of Staphylococcus aureus under the effect of batumin. Zh. Mikrobio.l Epidemiol. Immunobiol. 2013, V. 5, P. 3?8.
16. Gibson J., Sood A., Hogan D. Pseudomonas aeruginosa ? Candida albicans interactions: localization and fungal toxicity of a phenazine derivative A. and E. Microbiology. 2009, 75 (2), 504?513. https://doi.org/10.1128/AEM.01037-08
17. Sidrima J. J. C., Teixeira C. E. C., Cordeiro R. A., Brilhante R. S. N., Castelo-Branco D. S. C. M., Bandeira S., Alencar L. P., Oliveira J. S., Monteiro A. J., Moreira J. L. B., Bandeira T. J. P. G., Rocha M. F. G. ?-Lactam antibiotics and vancomycin inhibit the growth of planktonic and bio?lm Candida spp.: An additional bene?t of antibiotic-lock therapy. Int. J. Antimicrob. Agents. 2015, V. 45, P. 420–423. https://doi.org/10.1016/j.ijantimicag.2014.12.012
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- Hits: 385
ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
Biotechnologia Acta V. 13, No 3, 2020
Р. 64-72, Bibliography 24, English
Universal Decimal Classification: 615.322:616.37
https://doi.org/10.15407/biotech13.03.064
M. Kryvtsova 1, M. Hrytsyna 2, I. Salamon 3
1 Uzhhorod National University, Ukraine
2 Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies, Lviv, Ukraine
3 University of Pre?ov, Slovakia
Essential oils are widely used in beauty therapy, medicine and food industry, and they are considered to be a valuable consumer product. At the same time, the biochemical composition and properties of essential oils, including their antimicrobial activity, varies depending on the habitat, climatic conditions and plant chemotype. The purpose of our work was to study the qualitative and quantitative composition of essential oils and their antimicrobial properties, from Origanum vulgare plants harvested in eastern Slovakia and Lviv region, Ukraine.
In the wild, O. vulgare L. were gathered in close vicinity of the village of Trostianets, Lviv region, Ukraine, in 2019. In Slovakia, the plants were grown by Agrokarpaty Company, Plavnica. Essential oils were extracted by hydrodistillation (2 hours) in a Clevenger apparatus, according to the European pharmacopoeia.
The analysis of the biochemical properties of essential oils extracted from plant populations from Lviv region, showed that the contents of essential oils were within 0.35 ± 0.05%. The composition of essential oils shows that Origano vulgare L. plants from the natural population grown in Lviv region, belongs to the monoterpene chemotype. Monoterpene hydrocarbons ?-terpinene and ?-terpineol together accounted for 29?33%, acyclic monoterpenes – ?-myrcene – 7%, linalool – 4%, while the polyphenol compound p-cymene accounted for only 15%.
The O. vulgare plants from Slovakia were characterised by the essential oil content of 0.15 to 0.50%, and the composition which allowed us to refer them to the carvacrol chemotype, with phenols as its main ingredients – carvacrol and thymol (together 71%), and isopropyltoluene (4.0%). Monoterpene hydrocarbons terpinene (5.0%) and terpineol alcohol (6.0%) jointly accounted for 11%; acyclic monoterpene mycrene – 3%; and sesquiterpene ?-caryophyllene – 4,5%.
Essntial oil from O. vulgare harvested in Slovakia demonstrated high antimicrobial activity against reference and clinical isolates of opportunistic microorganisms. Essential oil from the samples gathered in Lviv region, showed low antimicrobial activity.
Thus, it has been shown that the reviewed plants referred to different chemotypes, which calls forth the prospect of the use of essential oils extracted from different plant chemotypes for different purposes, depending upon their biochemical composition and properties.
Key words: oregano essential oil, antimicrobial activity, biochemical properties
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2020
References
1. Shafiee-Hajiabad M., Novak J., Honermeier B. Characterization of glandular trichomes in four Origanum vulgare L. accessions influenced by light reduction. J. Appl. Botany and Food Quality. 2015, V. 88, P. 300?307. https://doi.org/10.5073/JABFQ.2015.088.043
2. Lukas B., Schmiderer C., Novak J. Essential oil diversity of European Origanum vulgare L. (Lamiaceae). Phytochem. 2015, V. 119, P. 32?40. https://doi.org/10.1016/j.phytochem.2015.09.008
3. Tuttolomondo T., Leto C., Leone R., Licata M., Virga G., Ruberto G., Edoardo M. Napoli, Salvatore La Bella. Essential oil characteristics of wild Sicilian oregano populations in relation to environmental conditions. J. Essential Oil Res. 2014, 26 (3), 210?220. https://doi.org/10.1080/10412905.2014.882278
4. De Martino L., De Feo V., Formisano C., Mignola E., Senatore F. Chemical Composition and Antimicrobial Activity of the Essential Oils from Three Chemotypes of Origanum vulgare L. ssp. hirtum (Link) Ietswaart Growing Wild in Campania (Southern Italy). Molecules. 2009, V. 14, P. 2735?2746. https://doi.org/10.3390/molecules14082735
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6. Jerkovi? I., Masteli? J., Milo? M. The impact of both the season of collection and drying on the volatile constituents of Origanum vulgare L. ssp. hirtum grown wild in Croatia. Inter. J. Food Sci. Technol. 2001, V. 36, P. 649?654. https://doi.org/10.1046/j.1365-2621.2001.00502.x
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8. Carrasco A., Perez E., Cutillas A.-B., Martinez-Gutierrez R., Tomas V., Tudela J. Origanum Vulgare and Thymbra Capitata Essential Oils from Spain: Determination of Aromatic Profile and Bioactivities. Natural Product Communications. 2016, V. 11, P. 113–120. https://doi.org/10.1177/1934578X1601100133
9. Mockute D., Bernotiene G., Judzentiene A. The essential oil of Origanum vulgare L. ssp. vulgare growing wild in Vilnius district (Lithuania). Phytochem. 2001, 57 (1), 65?69. https://doi.org/10.1016/S0031-9422(00)00474-X
10. Xiao-LiZhang, Yu-ShanGuo, Chun-HuaWang, Guo-QiangLi, Jiao-JiaoXu, Hau YinChung, Wen-CaiYe, Yao-LanLi, Guo-CaiWang. Phenol compounds from Origanum vulgare and their antioxidant and antiviral activities. Food Chem. 2016, 152 (1), 300?306. https://doi.org/10.1016/j.foodchem.2013.11.153
11. Kotyuk L. A., Rakhmeto D. B. Biolohichno aktyvni rechovyny Origanum vulgare L. Plant physiol. genetics. 2016, 48 (1), 20?25. (In Ukrainian). https://doi.org/10.15407/frg2016.01.020
12. Perez R. A., Navarro T., Lorenzo C. D. HS–SPME analysis of the volatile compounds from spices as a source of flavour in ‘Campo Real’table olive preparations. Flavour and fragrance j. 2007, 22 (4), 265?273. https://doi.org/10.1002/ffj.1791
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17. Johnson C. B., Kazantzis A., Skoula M., Mitteregger U., Novak J. Seasonal, populational and ontogenic variation in the volatile oil content and composition of individuals of Origanum vulgare subsp. Hirtum, assessed by GC headspace analysis and by SPME sampling of individual oil glands. Phytochem. Anal. 2004, V. 15, P. 286?292. https://doi.org/10.1002/pca.780
18. De Falco E., Roscigno G., Landolfi S., Scandolera E., Senatore F. Growth, essential oil characterization, and antimicrobial activity of three wild biotypes of oregano under cultivation condition in Southern Italy. Industrial Crops and Products. 2014, V. 62, P. 242–249. https://doi.org/10.1016/j.indcrop.2014.08.037
19. Mith H., Dure R., Delcenserie V., Zhiri A., Daube G., Clinquart A. Antimicrobial Activities of Essential Oils and Their Components against Food-Borne Pathogens and Food Spoilage Bacteria. Food Sci. Nutr. 2014, V. 2, P. 403?416. https://doi.org/10.1002/fsn3.116
20. De Azeredo G. A., Stamford T. L. M., Nunes P. C., Neto N. J. G., De Oliveira, De Souza E. L. Combined application of essential oils from Origanum vulgare L. and Rosmarinus officinalis L. to inhibit bacteria and autochthonous microflora associated with minimal.y processed vegetables. Food Res. Inter. 2011, 44 (5), 1541?1548. https://doi.org/10.1016/j.foodres.2011.04.012
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22. Salamon I., Poracova J., Hrytsyna M. Oregano Essential Oil (Origanum vulgare L.), as a Food-supplement in a Rearing of Piglets. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences. 2019, 21 (95), 55?61. https://doi.org/10.32718/nvlvet9510
23. Salamon I., Kryvtsova M., Bucko D., Tarawneh Amer H. Chemical characterization and antimicrobial activity of some essential oils after their industrial large-scale distillation. J. Microbiol. Biotechnol. Food Sci. 2018, 8 (3), 965?969.https://doi.org/10.15414/jmbfs.2018.8.3.965-969
24. Kryvtsova M. V., Kostenko Ye. Ya., Salamon I. Compositions of essential oils with antimicrobial properties against isolates from oral cavities of patients with inflammatory diseases of parodentium. Regulatory Mechanisms in Biosystems. 2018, 9 (4), 491?494. https://doi.org/10.15421/021873
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- Hits: 459
ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
Biotechnologia Acta V. 13, No 3, 2020
Р. 52-63, Bibliography 29, English
Universal Decimal Classification: 616-056.52+615.322
https://doi.org/10.15407/biotech13.03.052
G. V. Ostrovska 1, T. V. Krupska 2, L. M. Pazyuk 1, M. E. Dzerzhynsky 1, V. V. Turov 2
1 ESC "Institute of Biology and Medicine" of Taras Shevchenko Kyiv National University
2 Chuiko Institute of Surface Chemistry of the National Academy of Sciences of Ukraine, Kyiv
The features of the influence of the nanocomposite system (Lymphosilica supplement), which was created on the basis of hydroconsolidated finely divided silica and plant materials with a high content of polyphenols, flavonoids, terpenes, polysaccharides on liver cytophysiological characteristics under the conditions of the development of metabolic syndrome and hepatotoxicity under simulated alimentary, were studied. Biochemical studies of blood serum and histological analysis of liver tissue were performed. It was shown that a high-calorie diet of rats over 100 days leads to the development of individual manifestations of obesity and metabolic syndrome, which are expressed in an increase in body weight and visceral fat, the development of the initial and middle stages of parenchymal fatty liver dystrophy with a decrease in morphological manifestations of the synthetic activity of the nucleus, with slight deviations at the biochemical level. The introduction of a phytocomposite does not lead to pathological changes in the organism of rats, but in some cases leads to manifestations of “adaptive stress” of liver hepatocytes, both with a standard diet and with a high-calorie diet. The positive effect of the composite system on reducing the proportion of visceral fat (by 38%) in rats with a standard diet was shown, and against the background of simulated alimentary obesity, normalization of the level of bilirubin (25 % decrease compared with alimentary obesity) and its fractions, lower levels cholesterol and alkaline phosphatase (in both cases by 19% compared with initial obesity), increased globulin fractions of blood serum, protective effect against dystrophic changes in liver tissue.
Key words: nanocomposite, Lymphosilica, nanosilica, rats, alimentary obesity, hepatocytes of theliver, visceralfat.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2020
References
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- Details
- Category: 3_2020(en)
- Hits: 449
ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
Biotechnologia Acta V. 13, No 3, 2020
Р. 45-51, Bibliography 41, English
Universal Decimal Classification: 615.277.3+615.012]-022.532-092.9
https://doi.org/10.15407/biotech13.03.045
PREVENTION OF CISPLATIN TOXICITY AGAINST NORMAL CELLS BY COMPLEXATION WITH C60 FULLERENE
1 Taras Shevchenko National University of Kyiv, Ukraine
2 Palladin Institute of Biochemistry of NAS of Ukraine, Kyiv, Ukraine
The aim of this study was to evaluate the toxicity of noncovalent nanocomplex of C60 fullerene with cisplatin (C60-Cis-Pt) against normal cells. The toxicity of the C60-Cis-Pt nanocomplex compared to the free Cis-Pt was studied by estimating kidney human embryonic (HEK293) cells viability using MTT assay and rat erythrocytes resistance to acid haemolysis. It was shown that free 40 µM Cis-Pt changed the morphology and reduced the viability of HEK293 cells, as well as increased the number of haemolyzed erythrocytes compared to the According to the investigated parameters analysis no cytotoxic effects of C60-Cis-Pt nanocomplex was observed at Cis-Pt equivalent concentration. The prevention of Cis-Pt toxic action against normal cells by its complexation with C60 fullerene opens the prospect of nanostructure usage as an effective cytoprotector and a target carrier in tumor cells..
Key words: C60 fullerene, cisplatin, nanocomplex, НЕК293 cells, cytotoxicity, erythrocytes, haemolysis.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2020
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