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Home Archive 2019 № 6 GENETIC TRANSFORMATION OF PLANTS CONTAINING THE SYNTHETIC cry1Ab GENE ENCODING RESISTANCE TO LEPIDOPTERAN PESTS A. M. Taranenko, I. O. Nitovska, L. H. Velykozhon, P. D. Maystrov, M. V. Kuchuk, B. V. Morgun
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ISSN 2410-776X (Online)
ISSN 2410-7751 (Print)

"Biotechnologia Acta" V. 12, No 6, 2019
Р. 56-64 , Bibliography 29, English
Universal Decimal Classification: 577.21+632.78+57.084.1


A. M. Taranenko1, I. O. Nitovska1, L. H. Velykozhon1, 2, P. D. Maystrov1, M. V. Kuchuk1, B. V. Morgun1, 2

1Institute of Cell Biology and Genetic Engineeringof the National Academy of Sciences of Ukraine, Kyiv
2Institute of Plant Physiology and Genetics of thr National Academy of Sciences of Ukraine, Kyiv

The research was aimed to develop genetic constructs for Agrobacterium-mediated plant transformation, containing the synthetic cry1Ab gene, and their testing through the transformation of tobacco, followed by a molecular genetic analysis of the obtained plants to confirm the transformation event. Basic methods of DNA cloning, Agrobacterium-mediated transformation of Nicotiana tabacum L. by leaf disc method, selection of transformants in vitro, analysis of the transgene presence in plant DNA, detection of cry1Ab gene expression by PCR with reverse transcription were used. In the course of the study, the vectors pCB182 and pCB241 that contained the synthetic gene cry1Ab were constructed. Agrobacterium-mediated transformation of tobacco was carried out by created vectors and regenerant plants containing transgenes in their DNA were obtained. Expression of cry1Ab transgene in the obtained transformants of tobacco by the RT-PCR method was confirmed. As a result of the Agrobacterium-mediated transformation of plants with pCB182 and pCB241 vectors containing the synthetic cry1Ab lepidopteran resistance gene it is possible to obtain transgenic plants with expression of the transgene.

Key words: cry1Ab, Nicotiana tabacum L., Agrobacterium-mediated transformation, PCR-analysis, transgenesis.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2019

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Home Archive 2019 № 6 GENETIC TRANSFORMATION OF PLANTS CONTAINING THE SYNTHETIC cry1Ab GENE ENCODING RESISTANCE TO LEPIDOPTERAN PESTS A. M. Taranenko, I. O. Nitovska, L. H. Velykozhon, P. D. Maystrov, M. V. Kuchuk, B. V. Morgun

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