Biotechnologia Acta

...

  • Increase font size
  • Default font size
  • Decrease font size
Home Archive 2019 № 4 INFLUENCE OF NITROGEN CONCENTRATION ON PHOTOINDUCED GROWTH, ENZYMATIC ACTIVITY AND MELANINE SYNTHESIS BY Inonotus obliquus (Ach.:Pers.) Pilát Poyedinok N. L., Tugay T. I., Tugay A. V., Mykchaylova O. B.,Sergiichuk N. N., Negriyko A. M.
Print PDF

ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

"Biotechnologia Acta" V. 12, No 4, 2019
https://doi.org/10.15407/biotech12.04.034
Р. 34-41, Bibliography  20, English
Universal Decimal Classification:  579.22: 582.28

INFLUENCE OF NITROGEN CONCENTRATION ON PHOTOINDUCED GROWTH, ENZYMATIC ACTIVITY AND MELANINE SYNTHESIS BY Inonotus obliquus (Ach.:Pers.) Pilát

1Poyedinok N. L., 2Tugay T. I., 2Tugay A. V., 3Mykchaylova O. B.,4Sergiichuk N. N., 5Negriyko A. M.

1 Institute of Food Biotechnology and Genomicsof the  National Academy of Sciences of Ukraine, Kyiv
2 Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, Kyiv
3 Kholodny Institute of Botany of the National Academy of Sciences of Ukraine, Kiyv
4 Open International University of Human Development "Ukraine", Kyiv
5 Institute of Physics of the National Academy of Sciences of Ukraine, Kyiv

The aim of the work was to study the effect of nitrogen concentrations on photo-induction of growth, enzymatic activity and synthesis of melanin by the medicinal fungus Inonotus obliquus (Ach.: Pers.) Pilát from the Collection of cultures of cap mushrooms of  Kholodny Institute of Botany of the National Academy of Sciences of Ukraine. Irradiated by light of low intensity, different coherence and in different wavelength ranges, mycelium was cultivated in a dynamic mode on a glucose-peptone medium with different concentrations of total nitrogen. The concentration of the nitrogen source was not shown to significantly affect the photo-induced stimulation of the growth of I. obliquus. The increase in biomass accumulation of mycelium photoactivated in different modes was almost the same in all variants of the experiment, compared with the biomass of not-irradiated mycelium. A reliable dependence of the photo-stimulation of melanin synthesis on the concentration of nitrogen in the medium was established. Reduced nitrogen concentration more than twice increased the stimulating effect of low-intensity laser radiation with a wavelength of 488 nm. Using substrate with a reduced content of the nitrogen source is advisable to increase the photo-induced stimulating effect in the production of extracellular catalase, tyrosinase and polyphenol oxidase, intracellular peroxidase.

Thus, the cultivation parameters of I. obliquus and the light treatment regimes of the inoculum should be adjusted according to the target bioactive components.

Key words: Inonotus obliquus, photoinduction, nitrogen, melanin, catalase, tyrosinase, polyphenol oxidase, peroxidase, growth activity.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2019

  • References
    • 1. Zied D. C., Pardo-Giménez A. (Eds). Edible and Medicinal Mushrooms: Technology and Applications. Wiley & Sons Ltd. 2017, 562 р. https://doi.org/10.1002/9781119149446

      2. Chang S. T., Wasser S. P. The role of culinary-medicinal mushrooms on human welfare with a pyramid model for human health. Int. J. Med. Mushrooms. 2012, 14 (2), 95–134. https://doi.org/10.1615/IntJMedMushr.v14.i2.10

      3. Zheng W., Miao K., Liu Y., Zhao Y., Zhang M., Pan S., Dai Y. Chemical diversity of biologically active metabolites in the sclerotia of Inonotus obliquus and submerged culture strategies for up-regulating their production Appl. Microbiol. Biotechnol. 2010, 87 (4), 1237–1254. https://doi.org/10.1007/s00253-010-2682-4

      4. Zheng W., Zhao Y., Zheng X., Liu Y., Pan S., Dai Y., Liu F. Production of antioxidant and antitumor metabolites by submerged cultures of Inonotus obliquus cocultured with Phellinus punctatus. Appl. Microbiol. Biotechnol. 2011, 89 (1), 157–167. https://doi.org/10.1007/s00253-010-2846-2

      5. Poyedinok N. L. Light regulation of growth and melanin formation in Inonotus obliquus (Pers.) Pilat. Biotechnol. acta. 2013, 6 (2), 115−120. (In Russia). https://doi.org/10.15407/biotech6.02.115

      6. Poyedinok N. L., Mykchaylova O. B., Tugay T. I., Tugay A., Negriyko A., Dudka I. A. Effect of light wavelengths and coherence on growth, enzymes activity and melanin production of liquid cultured Inonotus obliquus (Ach.:Pers.) Pilát. Appl. Biochem. Biotechnol. 2015, 176 (2), 333−343. https://doi.org/10.1007/s12010-015-1577-3

      7. Friedl M. A., Kubicek C. P., Druzhinina I. S. Carbon source dependence and photostimulation of conidiation in Hypocrea atroviridis. Appl. Environ. Microbiol. 2008, 74 (1), 245−250. https://doi.org/10.1128/AEM.02068-07

      8. Friedl M. A., Schmoll M., Kubicek C. P., Druzhinina I. S. Photostimulation of Hypocrea atroviridis growth occurs due to a cross-talk of carbon metabolism, blue light receptors and response to oxidative stress. Microbiology. 2008, 154 (4), 1229–1241. https://doi.org/10.1099/mic.0.2007/014175-0

      9. Schuster A., Kubicek C. P., Friedl M. A., Druzhinina I. S., Schmoll M. Impact of light on Hypocrea jecorina and the multiple cellular roles of Envoy in this process. BMC genomics. 2007, 8 (1), 449. https://doi.org/10.1186/1471-2164-8-449

      10. Tisch D., Kubicek C. P., Schmoll M. The phosducin-like protein PhLP1 impacts regulation of glycoside hydrolases and light response in Trichoderma reesei. BMC genomics. 2011, 12 (1), 613. https://doi.org/10.1186/1471-2164-12-613

      11. Tisch D., Schmoll M. Targets of light signalling in Trichoderma reesei. BMC genomics. 2013, 14 (1), 657. https://doi.org/10.1186/1471-2164-14-657

      12. Tisch D., Kubicek C. P., Schmoll M. Crossroads between light response and nutrient signalling: ENV1 and PhLP1 act as mutual regulatory pair in Trichoderma reese. BMC genomics. 2014, 15 (1), 425–438. https://doi.org/10.1186/1471-2164-15-425

      13. Schmoll M. Light, stress, sex and carbon-the photoreceptor ENVOY as a central checkpoint in the physiology of Trichoderma reesei. Fungal Biol. 2017, 122 (6), 479–486. https://doi.org/10.1016/j.funbio.2017.10.007

      14. Zhu J. C., Wang X. J. Effect of blue light on conidiation development and glucoamylase enhancement in Aspergillus niger. Acta microbiologica Sinica. 2005, 45 (2), 275–278.

      15. Ricci M., Krappmann D., Russo V. E. A. Nitrogen and carbon starvation regulate conidia and protoperithecia formation of Neurospora crassa grown on solid media. Fungal. Genet. Newsl. 1991, 38 (1), 87–88. https://doi.org/10.4148/1941-4765.1462

      16. Sommer T., Degli-Innocenti F., Russo V. E. A. Role of nitrogen in the photoinduction of protoperithecia and carotenoids in Neurospora crassa. Planta. 1987, 170 (2), 205–208. https://doi.org/10.1007/BF00397889

      17. Poyedinok N. L., Mykchaylova O. B., Sergiichuk N. N., Negriyko A. M. Realization of Macromycete Photoinduced Growth Activity: Influence of Cultivation Ways and the Concentration of Carbon and Nitrogen. Innov. Biosyst. Bioeng. 2018, 2 (3), 196–202. https://doi.org/10.20535/ibb.2018.2.3.134629 (In Russia). https://doi.org/10.20535/ibb.2018.2.3.134629

      18. Mykchaylova O. B., Poyedinok N. L., Buchalo A. S. Biotechnological aspects of cultivation of species of the genus Morchella on liquid nutrient media. Immunopathology, Allergology, Infectology. 2009; 2 (1), 166−167. (In Russia).

      19. Puchkova T. A., Kapich A. N., Osadchaya O. V. Influence of conditions of cultivation for education biologically active substances by mushrooms of the genus Cordyceps and their antioxidant activity. Trudyi BGU. 2013, 8 (1), 246–252. (In Russia).

      20. Poyedinok N. L., Mykchaylova O. B., Buchalo A. S., Negriyko A. M. Light regulation of growth and biosynthetic activity of ling zhi or reishi medicinal mushroom, Ganoderma lucidum (W. Curt: Fr.) P. Karst. (Aphyllophoromycetideae) in pure culture. Int. J. Med. Mushr. 2008, 10 (4), 369–378. https://doi.org/10.1615/IntJMedMushr.v10.i4.100


 

Additional menu

Site search

Site navigation

Home Archive 2019 № 4 INFLUENCE OF NITROGEN CONCENTRATION ON PHOTOINDUCED GROWTH, ENZYMATIC ACTIVITY AND MELANINE SYNTHESIS BY Inonotus obliquus (Ach.:Pers.) Pilát Poyedinok N. L., Tugay T. I., Tugay A. V., Mykchaylova O. B.,Sergiichuk N. N., Negriyko A. M.

Invitation to cooperation

Dear colleagues, we invite you to publish your articles in our journal.
© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2008.
All rights are reserved. Complete or partial reprint of the journal is possible only with the written permission of the publisher.
E-mail
for information: biotech@biochem.kiev.ua.