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Home Archive 2019 № 3 INFLUENCE OF SHORT-WAVELENTH ULTRAVIOLET LIGHT ON GENES EXPRESSION IN Arabidopsis thaliana PLANTS M. Kryvokhyzha, Y. Libantova, N. Rashydov
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ISSN 2410-776X (Online)
ISSN 2410-7751 (Print)

"Biotechnologia Acta" V. 12, No 3, 2019
https://doi.org/10.15407/biotech12.03.057
Р. 57-66, Bibliography 29, English
Universal Decimal Classification: 637.03: 577.215

INFLUENCE OF SHORT-WAVELENTH ULTRAVIOLET LIGHT ON GENES EXPRESSION IN Arabidopsis thaliana PLANTS

M. Kryvokhyzha1, Y. Libantova2, N. Rashydov1

1Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine, Kiyv
2Institute Plant Genetics and Biotechnology of SAS, Slowak Republic

The aim of the work was to estimate the impact of the short wavelengths ultraviolet radiation (wavelength is 230 nm) on Arabidopsis thaliana. The stress response on some key flowering determination genes AP1, GI, LFY, FT, CO, and the repair gene RAD51 expression were investigated. The grown plants were applied by red (610–700 nm), violet (400–450 nm), neutral white (mixture wavelengths 380–750 nm), 20 V and high intensive white light (mixture wavelengths 380–750 nm) 40V LED. The experimental group of plants was irradiated by short wavelengths ultraviolet on ontogenesis stage 5.1 by Boyes classification. The leaf length as growth parameter mark also was analyzed. The short wavelengths ultraviolet influence caused differences in photoperiodic pathway genes expression in plants grown under different illumination. Acceleration flowering phases under influence white intensive illumination and delay ones in case of violet and common white illumination were observed comparing with control groups. It was revealed that cryptochrome and phytochrome formation play an important role in plant development and stress resistance. It enables to understand the best way of plant cultivation in stressful condition.

Key words: illumination conditions, gene expression, short wavelengths ultraviolet, stress response.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2018

  • References
    • 1. Seiler F., Soll J., Bölter B. Comparative phenotypical and molecular analyses of arabidopsis grown under fluorescent and LED light. Plants (Basel). 2017, 6 (2), pii: E24. https://doi.org/10.3390/plants6020024

      2. Song K., Kim H. Ch., Shin S., Kim K. H., Moon J. Ch., Kim J. Y., Lee1 B. M. Transcriptome analysis of flowering time genes under drought stress in maize leaves. Front. Plant Sci. 2017, 8 (267). https://doi.org/10.3389/fpls.2017.00267

      3. Valentim F., Mourik S., Posé D., Kim M., Schmid M., van Ham R., Busscher M., Sanchez-Perez G., Molenaar J., Angenent G., Immink R., van Dijk A. A quantitative and dynamic model of the Arabidopsis flowering time gene regulatory network. PLoS One. 2015, 10 (2), e0116973. https://doi.org/10.1371/journal.pone.0116973

      4. Song Y., Ito Sh., Imaizumi T. Flowering time regulation: in leaves. Trends in Plant Science. 2013, 18 (10), Elsevier Ltd: 575–583. https://doi.org/10.1016/j.tplants.2013.05.003

      5. Bernier G., Périlleux C. A physiological overview of the genetics of flowering time control. Plant Biotechnology Journal. 2005, 3 (1), 3–12. https://doi.org/10.1111/j.1467-7652.2004.00114.x

      6. McCree K. J. The action spectrum, absorptance and quantum yield of photosynthesis in crop plants. Agricultural Meteorology. 1972, 9 (3), 191–216.https://doi.org/10.1016/0002-1571(71)90022-7

      7. Cashmore A., Jarillo J., Wu Y., Liu D. Cryptochromes: blue light receptors for plants and animals. 1999, V. 284, P. 760–766. https://doi.org/10.1126/science.284.5415.760

      8. Dougher T., Bugbee B. Evidence for yellow light suppression of lettuce growth. Photochem Photobiol. 2001, 73 (2), 208–212. https://doi.org/10.1562/0031-8655(2001)073<0208:EFYLSO>2.0.CO;2

      9. Brown C., Schuerger A., Sager J. Growth and photomorphogenesis of pepper plants under red light-emitting diodes with supplemental blue or far-red lighting. J. Am. Soc. Hortic. Sci. 1995, 120 (5), 808–813. https://doi.org/10.21273/JASHS.120.5.808

      10. King R., Hisamatsu T., Goldschmidt E., Blundell C. The nature of floral signals in arabidopsis. I. Photosynthesis and a farred photoresponse independently regulate flowering by increasing expression of FLOWERING LOCUS T (FT). J. Exp. Bot. 2008, 59 (14), 3811–3820. https://doi.org/10.1093/jxb/ern231

      11. Yano A., Fujiwara K. Plant lighting system with five wavelength-band light-emitting diodes providing photon flux density and mixing ratio control. Plant Methods. 2012, V. 8, P. 46. https://doi.org/10.1186/1746-4811-8-46

      12. Liu B., Zuo Z., Liu H., Liu X., Lin C. Arabidopsis cryptochrome 1 interacts with SPA1 to suppress COP1 activity in response to blue light. Genes and Development. 2011, 25 (10), 1029–1034.https://doi.org/10.1101/gad.2025011

      13. Ahmad M., Jarillo J., Smirnova O., Cashmore R. Cryptochrome blue-light photoreceptors of Arabidopsis implicated in phototropism. Nature. 1998, V. 392, P. 720–723. https://doi.org/10.1016/1369-5266(88)80000-1

      14. Teramura A., Sullivan J. Effects of UV-B radiation on photosynthesis and growth of terrestrial plants. Photosynth Res. 1994, 39 (3), 463–473. https://doi.org/10.1007/BF00014599

      15. Rozema J., Lenssen G., Van De Staaij J., Tosserams М., Visser А., Broekman R. Effects of UV-B radiation on terrestrial plants and ecosystems: interaction with CO2 enrichment. Plant Ecology. 1997, V. 128, P. 183–191. https://doi.org/10.1023/A:1009762924174

      16. Hollo F. Effects of ultraviolet radiation on plant cells. Micron. 2002, 33 (2), 179–197. https://doi.org/10.1016/S0968-4328(01)00011-7

      17. Brem R., Guven M., Karran P. Oxidativelygenerated damage to DNA and proteins mediated by photosensitized UVA. Free Radical Biology and Medicine. 2017, V. 107, P. 101–109. https://doi.org/10.1016/j.freeradbiomed.2016.10.488

      18. Gasperini A., Sanchez S. Doiron A., Lyles M., German G. Non-ionising UV light increases the optical density of hygroscopic self assembled DNA crystal films. Scientific Reports, Springer US. 2017, P. 1–10.https://doi.org/10.1038/s41598-017-06884-8

      19. Fina J., Casadevall R., AbdElgawad H., Prinsen E., Markakis M., Beemster G., Casati P. UV-B inhibits leaf growth through changes in growth regulating factors and gibberellins levels. Plant Physiol. 2017, 74 (2), 1110–1126. https://doi.org/10.1104/pp.17.00365

      20. Sampson B., Cane J. Impact of enhanced ultraviolet-B radiation on flower, pollen, and nectar production. American Journal of Botany. 1999, 86 (1), 108–114. https://doi.org/10.2307/2656959

      21. The Arabidopsis Genome Initiative. Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature. 2000, 408 (6814), 796–815. https://doi.org/10.1038/35048692

      22. Boyes D. Growth stage-based phenotypic analysis of Arabidopsis: a model for high throughput functional genomics in plants. The plant cell online. 2001, 13 (7), 1499–1510. https://doi.org/10.1105/tpc.13.7.1499

      23. Logemann J., Schell J., Willmitzer L. Improved method for the isolation of RNA from plant tissues. Analytical Biochemistry. 1987, 163 (1), 16–20,https://doi.org/10.1016/0003-2697(87)90086-8

      24. Algina J., Olejnik S. Conducting power analyses for ANOVA and ANCOVA in between-subjects designs. Evaluation & the Health Professions. 2003, 26 (3), 288–314,https://doi.org/10.1177/0163278703255248

      25. Pfaffl M., Horgan G., Dempfle L. Relative expression software tool (REST) for groupwise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Research. 2002, 30 (9), e36.https://doi.org/10.1093/nar/30.9.e36

      26. D’Amico-Damiao V., Carvalho R. F. Cryptochrome-related abiotic stress responses in plants. Front Plant Sci. 2018, V. 9, P. 1897. https://doi.org/10.3389/fpls.2018.01897

      27. Litvinov S., Rashydov N. The transcriptional response of Arabidopsis thaliana L. genes AtKu70, AtRAD51 and AtRad1 to X-ray radiation. Journal of Agricultural Science and Technology. 2017, V. 7, P. 52–60. https://doi.org/10.17265/2161-6256/2017.01.008

      28. Muneer S., Park Y. G, Jeong B. R. Red and Blue Light Emitting Diodes (LEDs) Participate in Mitigation of Hyperhydricity in In Vitro-Grown Carnation Genotypes (Dianthus Caryophyllus). Journal of Plant Growth Regulation. 2018, 137 (2), 370–379. https://doi.org/10.1007/s00344-017-9733-3

      29. Xie X., He Z., Chen N., Tang Z., Wang Q., Cai Y. The roles of environmental factors in regulation of oxidative stress in plant. BioMed Research International. 2019. Article ID 9732325:11.https://doi.org/10.1155/2019/9732325