Biotechnologia Acta

...

  • Increase font size
  • Default font size
  • Decrease font size
Home Statement of Informed Consent PHYTOCHEMICAL SCREENING OF POLYHERBAL COMPOSITION BASED ON Portulaca oleracea AND IT’S EFFECT ON MACROPHAGE OXIDATIVE METABOLISM M. Gahramanova, R. Dovhyi, M. Rudyk, O. Molozhava, V. Svyatetska, L. Skivka

PHYTOCHEMICAL SCREENING OF POLYHERBAL COMPOSITION BASED ON Portulaca oleracea AND IT’S EFFECT ON MACROPHAGE OXIDATIVE METABOLISM M. Gahramanova, R. Dovhyi, M. Rudyk, O. Molozhava, V. Svyatetska, L. Skivka

Print PDF

Ж-л "Biotechnologia Acta" Т. 12, № 2, 2019
https://doi.org/10.15407/biotech12.02.056 

PHYTOCHEMICAL SCREENING OF POLYHERBAL COMPOSITION BASED ON Portulaca oleracea AND IT’S EFFECT ON MACROPHAGE OXIDATIVE METABOLISM

M. Gahramanova, R. Dovhyi, M. Rudyk, O. Molozhava, V. Svyatetska, L. Skivka

Abstract

The aim of the work was to explore phytochemical characteristics of water extract from polyherbal composition based on P. oleracea and it’s effect on oxidative metabolism of murine peritoneal macrophages. The qualitative phytochemical analysis was conducted by colorimetric method. Quantitative analysis of phenols was performed in the test with gallic acid as a standard. Murine peritoneal macrophages were isolated without previous sensitization. Leukotoxicity of the water extract from polyherbal composition leukotoxicity was evaluated in MTT test. Reactive oxygen species generation was assayed by the nitroblue tetrazolium reduction method. Phytochemical analysis revealed the presence of water-soluble and insoluble phenols, tannins, saponins, flavonoids, cardiac glycosides and coumarins in the studied plant mixture. The water extract from polyherbal composition used in a range of concentration 1–1000 μg/ml (lyophilisate in distilled H2O) didn’t exhibit any toxic effects on murine peritoneal macrophages. Water extract from polyherbal composition caused statistically significant dose-dependent increase in oxidative metabolism of murine peritoneal suggest modulatory effect of studied water extract from polyherbal composition on innate immunity cells.

Full text PDF

 


 

Additional menu

Site search

Site navigation

Home Statement of Informed Consent PHYTOCHEMICAL SCREENING OF POLYHERBAL COMPOSITION BASED ON Portulaca oleracea AND IT’S EFFECT ON MACROPHAGE OXIDATIVE METABOLISM M. Gahramanova, R. Dovhyi, M. Rudyk, O. Molozhava, V. Svyatetska, L. Skivka

Invitation to cooperation

Dear colleagues, we invite you to publish your articles in our journal.
© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2008.
All rights are reserved. Complete or partial reprint of the journal is possible only with the written permission of the publisher.
E-mail
for information: biotech@biochem.kiev.ua.