Biotechnologia Acta

...

  • Increase font size
  • Default font size
  • Decrease font size
Home Archive 2019 № 2 INDUCTION OF CAROTENOGENESIS IN Desmodesmus armatus (Chod.) Hegew CULTIVATED ON THE WASTE WATER FROM RECIRCULATING AQUACULTURE SYSTEM M. M. Marchenko, I. V. Dorosh, L. M. Cheban
Print PDF

ISSN 2410-776X (Online)
ISSN 2410-7751 (Print)

"Biotechnologia Acta" V. 12, No 2, 2019
https://doi.org/10.15407/biotech12.02.046
Р. 46-55, Bibliography 32, English
Universal Decimal Classification:  582.263.1:[604.2:547.979.8]

INDUCTION OF CAROTENOGENESIS IN Desmodesmus armatus (Chod.) Hegew CULTIVATED ON THE WASTE WATER FROM RECIRCULATING AQUACULTURE SYSTEM

M. M. Marchenko, I. V. Dorosh, L. M. Cheban

Yuriy Fedkovych Chernivtsi National University

The aim of the study was to develop the biotechnology approach for obtaining secondary carotenoids of green microalgae Desmodesmus armatus (Chod.) Hegew. under conditions of two-stage cultivation on waste water from recirculating aquaculture system in response to the action of inducers of different origin. By chemical nature, secondary carotenoids are C40-ketocarotinoids - intermediates of enzymatic oxidation of β-carotene to astaxanthin.

The study presents the conditions for cultivation of D. armatus on the waste water from the recirculating aquaculture system by a two-stage accumulation process, where conditions for rapid growth of biomass were created at the first stage, and the biosynthesis of the target product was induced by the introduction of carotenoid biosynthesis precursors (C6H12O6, CH3COONa), promoters of free radical oxidation (FeSO4 / H2O2) or osmotic stress (NaCl) into the nutrient medium. It was shown that the first phase of cultivation was characterized by high growth and productive indices: the amount of biomass was up to 13 g/l, the content of total proteins was 37.9 %, lipids – 26 % and total carotenoids – 7.5 % per gram of dry biomass. Among carotenoids, the presence of zeaxanthin, lutein, β-carotene, insignificant amounts of astaxanthin, canthaxanthin, esters of adonixanthin and astaxanthin were detected. The features of the adaptive response of D. armatus to the influence of factors that induce secondary carotenogenesis are established. Among them is retention of the number of cells or doubling of their number during the use of chemical activators. Decrease in the activity of cytochrome oxidase as an indicator of the metabolic activity of the culture.

Thus, the possibility of increasing the content of β-carotene and astaxanthin in D. armatus biomass, essential for fish and crustaceans, by introducing promoters of free radical oxidation and osmotic stress NaCl (200 mM) or Fe2+ (200 mM) and H2O2 (10–4 mM) into the waste water from RAS in the second phase of cultivation was established. Metabolic disbalance in D. armatus cells, which were observed under the influence of chemical factors, led to a redistribution of the main nutrients profile. Biosynthesis and accumulation of lipids were activated against the background of intensive carotenogenesis.

Key words: D. esmodesmus armatus, two-stage accumulative cultivation, recirculating aquaculture system RAS, secondary carotenoids.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2019

  • References
    • 1. Urmych E. M., Berdikulov H. A., Eshpulatova M. B. The productivity of microalgae in intensive culture conditions. Algology. 2008, 18 (2), 111–117. (In Ukrainian).

      2. Zolotareva O., Shnyukova E., Sivash O., Mihaylenko N. Prospects of use of microalgae in biotechnology. Kyiv: Alterpres. 2008, 234 p. (In Ukrainian).

      3. Chatzifotis S., Pavlidis M., Jimeno C., Vardanis G., Sterioti A., Divanach P. The effect of different carotenoid sources on skin coloration of cultured red porgy (Pagrus pagrus). Aquacult. Res. 2005, V. 36, P. 1517–1525.

      4. Minerva G. C., Maurilio L. F. The use of carotenoid in aquaculture. Res. J. Fisher. Hydrobiol. 2013, 8 (2), 38–49.

      5. Arnaud M. F. The role of microalgae in aquaculture: situation and trends. J. Appl. Phycol. 2000, 12 (3–5), 527–534.

      6. Hemaiswarya S., Raja R., Ravi R. Kumar, Ganesan V., Anbazhagan C. Microalgae: a sustainable feed source for aquaculture. World J. Microbiol. Biotechnol. 2011, 27 (8), 1737–1746.

      7. Ladygina L. V. Carotenoid composition in microalgae for larvae of bivalve mollusks. Algology. 2010, 20 (1), 33–41. (In Russian).

      8. Raja R., Hemaiswarya S., Rengasamy R. Exploitation of Dunaliella for -carotene production. Appl. Microbiol. Biotechnol. 2007, V. 74, P. 517–523.

      9. Lorenz R. T., Cysewski G. R. Commercial potential for Haematococcus microalgae as a natural source of astaxanthin. Tibtech April. 2000, V. 18, P. 160–167.

      10. El-Sayed A. B. Carotenoids Accumulation in the Green Alga Scenedesmus sp. Incubated with Industrial Citrate Waste and Different Induction Stresses. Nat. Scie. 2010, 8 (10), 34–40.

      11. Guedes A. C., Amaro H. M., Malcata F. X. Microalgae as Sources of Carotenoids. Mar. Drugs. 2011, 9 (4), 625–644

      12. Маrchenko М. М., Cheban L. М., Grynko O. E., Khudyi О. І., Kushniryk J. V., Khuda L. V., Dorosh І. V. The method of growing Daphnia magna (Srtaus, 1820) is compatible with the fodder substrate (algae). Ukrainе. Pat. № 121772. 11. 12. 2017. (In Ukrainian).

      13. Lemoine Y., Schoefs B. Secondary ketocarotenoid astaxanthin biosynthesis in algae: a multifunctional response to stress. Photosynth. Res. 2010, V. 13, P. 155–157.

      14. Sarada R., Tripathi U., Ravishankar G. A. Influence of stress on astaxanthin production in Haematococcus pluvialis grown under different culture conditions. Proc. Biochem. 2002, V. 37, P. 623–627.

      15. Minyuk G. S., Chelebieva E. S., Chubchikova I. N. Special features in the secondary carotenogenesis Bracteacoccus minor (Chlorophyta) in a two-stage culture. Algologia. 2015, 25 (1), 21–34. (In Russian).

      16. Маrchenko М. М., Khudyi О. І., Cheban L. М., Khuda L. V., Malishchuk І. V. The method of culturing phytoplankton. Ukrainе. Pat. № 101103. 25. 08. 2015. (In Ukrainian).

      17. Cheban L., Malischuk I., Marchenko M. Cultivating Desmodesmus armatus (Chod.) Hegew. in recirculating aquaculture systems (RAS) waste water. Arch. Pol. Fish. 2015, V. 23, P. 155–162.

      18. Gudvilovich I. N., Gorbunova S. Y., Lelekov A. S., Borovkov A. B. Production characteristics of quasi-continuous culture Porphyridium purpureum (Bory) Ross of environment in partial refund. Bulletin of the Nikitsky botanical garden. 2011, V. 103, P. 116–120. (In Russian).

      19. Vasilenko O. V., Bodnar O. I., Winiarskaya G. B., Sinyuk Y. V., Grubinko V. V. Energy and nitrogen metabolism in Chlorella vulgaris Beij. (Chlorophyta) the influence of sodium gypsum. Algologia. 2014, 24 (3), 297–301. (In Ukrainian).

      20. Gonzalez Lopez C. V., Ceron Garcia M., Acien Fernandez F. G., Bustos C. S., Chisti Y., Fernandez Sevilla J. M. Protein measurements of microalgal and cyanobacterial biomass. Bioresource Technol. 2010, P. 7587–7591.

      21. Knigh J. A., Anderson S., Rawle J. M. Chemical basis of the sulfo-phosphovanilin reaction for estimating total serum lipids. Clin. Chem. 1972, P. 199–202.

      22. Oturina I. P., Makarova E. I., Sidyakin A. I. Features of dynamics of the main photosynthetic pigments and biomass accumulation in microalgae Scenedesmus sp. — representative mikroalgoflory freshwater ekosistem. Ekosistemy, their optimization and security. 2010, V. 2, P. 84–91. (In Russian).

      23. Dere S., Tohit G., Sivaci R. Spectrophotometric Determination of Chlorophyll — A, B and Total Carotenoid Contents of Some Algae Species Using Different Solvents. Tr. J. Botany. 1998, V. 22, P. 13–17.

      24. The State Pharmacopoeia of Ukraine. Kyiv, 2012 p. (In Ukrainian).

      25. Chelebieva E. S., Minyuk G. S., Chubchykova I. M. Features of the secondary carotenogenesis in green microalgae Scenedesmus rubescens (Dangeard) Kessler et al. two phasic conditions in batch culture. Scientific notes of Taurida National University. V. I. Ver nadsky series «Biology and chemistry». 2013, 26 (65), 4, 175–187. (In Russian).

      26. Zhang D. H., Lee Y. K. Two-Step process for ketocarotenoid production by a green Аlga Chlorococcum sp. Strain MA-1 Appl. Мicrobiol. Biotechnol. 2001, V. 55, P. 537–540.

      27. Solovchenko A. E., Chivkunova O. B., Smirnov L. R., Selyah I. O., Shcherbakov P. N., Karpova E. A., Lobakova E. S. Effects of stress on pigment content of lipids and fatty acids in cells of microalgae Desmodesmus sp. Of byelomorsk hydroids. Physiol. Plants. 2013, 60 (3), 320–329. (In Russian).

      28. Solovchenko А. E. Physiology and adaptive significance of secondary carotenogenesis the green microalgae. Phisiol. Plant. 2013, V. 60, P. 3–16. (In Russian).

      29. Fraser P. D., Bramley P. M. The biosynthesis and nutritional uses of Carotenoids. Progr. Lipid Res. 2004, V. 43, P. 228–265.

      30. Solovchenko А. E. The physiological role of the accumulation of neutral lipids eukaryotic microalgae under stress. Phisiol. Plant. 2012, V. 59, P. 192–202. (In Russian).

      31. Yap C. Y. Chen F. Polyunsaturated fatty acids: biological significance, biosynthesis and production by microalgae and microalgae-like organisms. Algae Biotechnol. Potent. 2001, 1 (32), 32 p.

      32. Katz A., Jimenez C., Pick U. Isolation and Characterization of a Protein Associated with Carotene Globules in the Alga Dunaliella bardawil. Plant Physiol. 1995, V. 108, P. 1657–1664


 

Additional menu

Site search

Site navigation

Home Archive 2019 № 2 INDUCTION OF CAROTENOGENESIS IN Desmodesmus armatus (Chod.) Hegew CULTIVATED ON THE WASTE WATER FROM RECIRCULATING AQUACULTURE SYSTEM M. M. Marchenko, I. V. Dorosh, L. M. Cheban

Invitation to cooperation

Dear colleagues, we invite you to publish your articles in our journal.
© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2008.
All rights are reserved. Complete or partial reprint of the journal is possible only with the written permission of the publisher.
E-mail
for information: biotech@biochem.kiev.ua.