Biotechnologia Acta


  • Increase font size
  • Default font size
  • Decrease font size
Home Archive 2019 № 2 PHYTOCHEMICAL SCREENING OF POLYHERBAL COMPOSITION BASED ON Portulaca oleracea AND IT’S EFFECT ON MACROPHAGE OXIDATIVE METABOLISM M. Gahramanova, R. Dovhyi, M. Rudyk, O. Molozhava, V. Svyatetska, L. Skivka
Print PDF

ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

"Biotechnologia Acta" V. 12, No 2, 2019
Р. 63-70, Bibliography 46, English
Universal Decimal Classification:  571.27: 581.6


M. Gahramanova1, 2, R. Dovhyi2, M. Rudyk2, O. Molozhava2, V. Svyatetska2, L. Skivka2

1Nargiz Medical Center, Baku, Azerbaijan
2Education Scientific Center “Institute of Biology and Medicine”,Taras Shevchenko National University of Kyiv, Ukraine

The aim of the work was to explore phytochemical characteristics of water extract from polyherbal composition based on P. oleracea and it’s effect on oxidative metabolism of murine peritoneal macrophages. The qualitative phytochemical analysis was conducted by colorimetric method. Quantitative analysis of phenols was performed in the test with gallic acid as a standard. Murine peritoneal macrophages were isolated without previous sensitization. Leukotoxicity of the water extract from polyherbal composition leukotoxicity was evaluated in MTT test. Reactive oxygen species generation was assayed by the nitroblue tetrazolium reduction method. Phytochemical analysis revealed the presence of water-soluble and insoluble phenols, tannins, saponins, flavonoids, cardiac glycosides and coumarins in the studied plant mixture. The water extract from polyherbal composition used in a range of concentration 1–1000 ?g/ml (lyophilisate in distilled H2O) didn’t exhibit any toxic effects on murine peritoneal macrophages. Water extract from polyherbal composition caused statistically significant dose-dependent increase in oxidative metabolism of murine peritoneal suggest modulatory effect of studied water extract from polyherbal composition on innate immunity cells.

Key words: water extract from polyherbal composition Portulaca oleracea, peritoneal macrophages, reactive oxygen species.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2019

  • References
    • 1. Venkatalakshmi P., Vadivel V., Brindha P. Role of phytochemicals as immunomodulatory agents: a review. Int. J. Green Pharm. 2016, 10 (1).

      2. Li F. S., Weng J. K. Demystifying traditional herbal medicine with modern approach. Nat. Plants. 2017, V. 3, P. 17109.

      3. Leonti M., Verpoorte R. Traditional Mediterranean and European herbal medicines. J. Ethnopharmacol. 2017, V. 199, P. 161–167.

      4. Iranshahy M., Javadi B., Iranshahi M., Jahanbakhsh S. P., Mahyari S., Hassani F. V. A review of traditional uses, phytochemistry and pharmacology of Portulaca oleracea L. J. Ethnopharmacol. 2017, V. 205, P. 158–172.

      5. Catap E. S., Kho M. J. L., Jimenez M. R. R. In vivo nonspecific immunomodulatory and antispasmodic effects of common purslane (Portulaca oleracea Linn.) leaf extracts in ICR mice. J. Ethnopharmacol. 2018, V. 215, P. 191–198.

      6. Li C. Y., Meng Y. H., Ying Z. M., Xu N., Hao D., Gao M., Zhang W. J., Xu L., Gao Y. C., Stien D., Ying X. X. Correction to Three Novel Alkaloids from Portulaca oleracea L. and Their Anti-inflammatory Effects. J. Agric. Food Chem. 2017, 65 (4), 993–994.

      7. Park Y. M., Lee H. Y., Kang Y. G., Park S. H., Lee B. G., Park Y. J., Lee H. M., Kim O. J., Yang H. J., Kim M. J., Lee Y. R. Immuneenhancing effects of Portulaca oleracea L.-based complex extract in cyclophosphamide-induced splenocytes and immunosuppressed rats. Food Agric. Immunol. 2019, 30 (1), 13–24.

      8. Dey A., Allen J., Hankey-Giblin P. A. Ontogeny and polarization of macrophages in inflammation: blood monocytes versus tissue macrophages. Front Immunol. 2015, V. 5, P. 683.

      9. Zhao Y., Zou W., Du J., Zhao Y. The origins and homeostasis of monocytes and tissue-resident macrophages in physiological situation. J. Cell Physiol. 2018, 233 (10), 6425–6439.

      10. Dev S. K., Choudhury P. K., Srivastava R., Sharma M. Antimicrobial, anti-inflammatory and wound healing activity of polyherbal formulation. Biomed. Pharmacother. 2018, V. 111, P. 555–567.

      11. Moukette B. M., Ama Moor V. J., Biapa Nya C. P., Nanfack P., Nzufo F. T., Kenfack M. A., Ngogang J. Y., Pieme C. A. Antioxidant and Synergistic Antidiabetic Activities of a Three-Plant Preparation Used in Cameroon Folk Medicine. Int. Sch. Res. Notices. 2017, V. 2017, P. 9501675.

      12. Yuan H., Ma Q., Cui H., Guancheng L., Zhao X., Li W., Piao G. How can synergism of traditional medicines benefit from network pharmacology? Molecules. 2017, V. 22, P. E1135.

      13. Deyab M., Elkatony T., Ward F. Qualitative and Quantitative Analysis of Phytochemical Studies on Brown Seaweed, Dictyota Dichotoma. IJEDR. 2016, 4 (2), 674–678.

      14. Djipa C. D., Delm?e M., Quetin-Leclercq J. Antimicrobial activity of bark extracts of Syzygium jambos (L.) Alston (Myrtaceae). J. Ethnopharmacol. 2000, V. 71, P. 307–313.

      15. Duraipandiyan V., Ayyanar M., Ignacimuthu S. Antimicrobial activity of some ethnomedicinal plants used by Paliyar tribe from Tamil Nadu, India. BMC Complement. Altern. Med. 2006, V. 6, P. 35.

      16. Oyedapo O. O., Sab F. C., Olagunju J. A. Bioactivity of fresh leaves of Lantana camara. Biomed. Lett. 1999, V. 59, P. 175–183.

      17. Cock I. E., Kukkonen L. An examination of the medicinal potential of Scaevola spinescens: Toxicity, antibacterial, and antiviral activities. Pharmacognosy Res. 2011. 3 (2), 85–94.

      18. Siddhuraju P., Becker K. The antioxidant and free radical scavenging activities of processed cowpea (Vigna unguiculata (L.) Walp.) seed extract. Food Chem. 2007, V. 101, P. 10–19.

      19. Fedorchuk O., Susak Y., Rudyk M., Senchylo N., Khranovska N., Skachkova O., Skivka L. Immunological hallmarks of cis-DDPExperimental articles 69 resistant Lewis lung carcinoma cells. Cancer Chemother. Pharmacol. 2018, 81 (2), 373–385.

      20. Jang H. H., Cho S. Y., Kim M. J., Kim J. B., Lee S. H., Lee M. Y., Lee Y. M. Antiinflammatory effects of Salvia plebeia R. Br extract in vitro and in ovalbumin-induced mouse model. Biol. Res. 2016, 49 (1), 41.

      21. Verma P. K., Raina R., Agarwal S., Kour H. Phytochemical ingredients and pharmacological potential of Calendula officinalis Linn. Pharm. Biomed. Res. 2018, 4 (2), 1–17.

      22. Aghili T., Arshami J., Tahmasbi A., Haghparast A. Effects of Hypericum perforatum extract on IgG titer, leukocytes subset and spleen index in rats. Effects of Hypericum perforatum extract on IgG titer, leukocytes subset and spleen index in rats. Avicenna J. Phytomed. 2014, 4 (6), 413–419.

      23. Lee B. R., Lee J. H., An H. J. Effects of Taraxacum officinale on fatigue and immunological parameters in mice. Molecules. 2012, V. 17, P. 13253–1326.

      24. Gupta V., Mittal P., Khokra S. I., Kaushik D. Pharmacological potential of Matricaria recutita: a review. Int. J. Pharmaceut. Sci. Drug Res. 2010, 2 (1), 12–16.

      25. Nkuete A. H. L., Migliolo L., Wabo H. K., Tane P., Franco O. L. Evaluation of multiple functions of Polygonum genus compounds. Eur. J. Med. Plant. 2015, 6 (1), 1–16.

      26. George A., Chinnappan S., Choudhary Y., Bommu P., Sridhar M. Immunomodulatory activity of an aqueous extract of Polygonum minus huds on Swiss albino mice using carbon clearance assay. Asian Pac. J. Trop. Dis. 2014, 4 (5), 398–400.

      27. Adel M., Abedian Amiri A., Zorriehzahra J., Nematolahi A., Esteban M. ?. Effects of dietary peppermint (Mentha piperita) on growth performance, chemical body composition and hematological and immune parameters of fry Caspian white fish (Rutilus frisii kutum). Fish Shellfish Immunol. 2015, 45 (2), 841–847.

      28. Arab Ameri S., Samadi F., Dastar B., Zerehdaran S. Effect of Peppermint (Mentha piperita) Powder on Immune Response of Broiler Chickens in Heat Stress. Iran. J. Appl. Anim. Sci. 2016, 6 (2), 435–445.

      29. Cosentino M., Bombelli R., Conti A., Colombo M. L., Azzetti A., Bergamaschi A., Marino F., Lecchini S. Antioxidant properties and in vitro immunomodulatory effects of peppermint (Menthax piperita L.) Essential oils in human leukocytes. J. Pharm. Sci. & Res. 2009, 1 (3), 33–43.

      30. Ding S., Jiang H., Fang J. Regulation of Immune Function by Polyphenols. J. Immunol. Res. 2018, V. 2018, P. 1264074.

      31. Murray P. J. Macrophage polarization. Ann. Rev. Physiol. 2017, V. 79, P. 541–566.

      32. Okwu D. E., Okwu M. E. Chemical composition of Spondias mormbia Linn plant parts. J. Sust. Agric. Eviron. 2004, V. 6, P. 140–147.

      33. Punturee K., Wild C. P., Kasinrerk W., Vinitketkumnuen U. Immunomodulatory activities of Centella asiatica and Rhinacanthus nasutus extracts. Asian Pac. J. Cancer Prev. 2005, V. 6, P. 396–400.

      34. Rajput Z. I., Hu S. H., Xiao C. W., Arijo A. G. Adjuvant effects of saponins on animal immune responses. J. Zhejiang Univ. Sci. B. 2007, 8 (3), 153–161.

      35. Akbay P., Basaran A. A., Undeger U., Basaran N. In vitro immunomodulatory activity of flavonoid glycosides from Urtica dioica L. Phytother. Res. 2003, V. 17, P. 34–37.

      36. Chiang L. C., Ng L. T., Chiang W., Chang M. Y., Lin C. C. Immunomodulatory activities of flavonoids, monoterpenoids, triterpenoids, iridoid glycosides and phenolic compounds of Plantago species. Planta Med. 2003, V. 69, P. 600–604.

      37. Yadav V. S., Mishra K. P., Singh D. P., Mehrotra S., Singh V. K. Immunomodulatory effects of curcumin. Immunopharmacol. Immunotoxicol. 2005, V. 27, P. 485–497.

      38. Abd-Alla H. I., Moharram F. A., Gaara A. H., El-Safty M. M. Phytoconstituents of Jatropha curcas L. leaves and their immunomodulatory activity on humoral and cell-mediated immune response in chicks. Z. Naturforsch. C. 2009, V. 64, P. 495–501.

      39. Ye Y., Li X., Sun H., Chen F., Pan Y. Immunomodulating Steroidal Glycosides from the Roots of Stephanotis mucronata. Helvetica Chimica Acta. 2004, V. 87, P. 2378–2384.

      40. Cherng J. M., Chiang W., Chiang L. C. Immunomodulatory activities of common vegetables and spices of Umbelliferae and its related coumarins and flavonoids. Food. Chem. 2008, 106 (3), 944–950.

      41. Galati G., O’Brien P. J. Potential toxicity of flavonoids and other dietary phenolics: significance for their chemopreventive and anticancer properties. Free Radic. Biol Med. 2004, 37 (3), 287–303.

      42. Bode A. M., Dong Z. Toxic phytochemicals and their potential risks for human cancer. Cancer. Prev. Res. (Phila). 2015, 8 (1), 1–8.

      43. Abais J. M., Xia M., Zhang Y., Boini K. M., Li P. L. Redox regulation of NLRP3 inflammasomes: ROS as trigger or effector? Antioxid. Redox Signal. 2015, 22 (13), 1111–1129.

      44. Zhang J., Wang X., Vikash V., Ye Q., Wu D., Liu Y., Dong W. ROS and ROS-mediated cellular signaling. Oxid. Med. Cell Longev. 2016, V. 2016, P. 4350965.

      45. Dunnill C., Patton T., Brennan J., Barrett J., Dryden M., Cooke J., Leaper D., Georgopoulos N. T. Reactive oxygen species (ROS) and wound healing: the functional role of ROS and emerging ROS-modulating technologies for augmentation of the healing process. Int. Wound. J. 2017, 14 (1), 89–96.

      46. Lee M. T., Lin W. C., Yu B., Lee T. T. Antioxidant capacity of phytochemicals and their potential effects on oxidative status in animals — A review. Asian-Australas J. Anim. Sci. 2017, 30 (3), 299–308.


Additional menu

Site search

Site navigation

Home Archive 2019 № 2 PHYTOCHEMICAL SCREENING OF POLYHERBAL COMPOSITION BASED ON Portulaca oleracea AND IT’S EFFECT ON MACROPHAGE OXIDATIVE METABOLISM M. Gahramanova, R. Dovhyi, M. Rudyk, O. Molozhava, V. Svyatetska, L. Skivka

Invitation to cooperation

Dear colleagues, we invite you to publish your articles in our journal.
© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2008.
All rights are reserved. Complete or partial reprint of the journal is possible only with the written permission of the publisher.
for information: