Biotechnologia Acta

...

  • Increase font size
  • Default font size
  • Decrease font size
Home Archive 2019 № 1 RAPE SWITCHGRASS BIOMASS (Brassica napus) AS RAW MATERIALS FOR BIOBUTANOL PRODUCTION O. Tigunova, G. Andrijash, N. Beyko, O. Zaharova, S. Priyomov, S. Shulga
Print PDF

ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

no1_2019.jpg - 961.57 Kb

"Biotechnologia Acta" V. 12, No 1, 2019
https://doi.org/10.15407/biotech12.01.075 
Р. 75-80, Bibliography 13, English
Universal Decimal Classification: 579.222

RAPE BIOMASS (Brassica napus) AS RAW MATERIALS
FOR BIOBUTANOL PRODUCTION

O. Tigunova1, G. Andrijash1,  N. Beyko1,  O. Zaharova2,
S. Priyomov1, S. Shulga
1

1SI “Institute of Food Biotechnology and Genomics”of the National Academy of Sciences of Ukraine, Kyiv
2 National University of Life and Environmental Sciences of Ukraine, Kyiv

The aim of the work was to investigate the accumulation of butanol by Clostridium strain producers using meshed green rape biomass as substrate. The accumulation of butanol by producer strains was studied using rape as substrate in the doses of 5–30 g/l. The cells were precipitated in an ultracentrifuge, the supernatant was distilled, and fermentation products were determined. The presence of solvents in the culture fluid was determined by gas chromatography. The biggest accumulation of butanol was produced by the strain Clostridium sp. IMB B-7570 on 2.3 g/l mashed rape biomass. The optimal inoculum concentration for maximum accumulation of butanol using rape biomass was 10% of the volume of fermentation liquid. The greatest accumulation of butanol (2.9 g/l) was obtained in optimal culture conditions and at 10 g/l dry rape biomass in the fermentation medium. Thus, the present study showed that mashed rape biomass was assimilated by Clostridium sp. strains. The accumulation of butanol depended of Clostridium strain, the amount of inoculum, the concentration and degree of grinding of the substrate.

Key words: biobutanol, Clostridium, plant biomass, rape.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2019

  • References
    • 1. Popa V. I. Biomass for fuel and biomaterials. Biomass as renewable raw material to obtain bioproducts of high-tech value.  “Gheorghe Asachi” Romania. 2018, P. 1–37. https://doi.org/10.1016/B978-0-444-63774-1.00001-6

      2. Balcerek M.,  Robak K. Review of second generation bioethanol production from residual biomass. Food Technol. Biotechnol. 2018, 56 (2), 174–187. https://doi.org/10.17113/ftb.56.02.18.5428

      3. Kusi O. A., Premjet D., Premjet S. Review Article A Review Article of Biological Pre-Treatment of Agricultural Biomass. Pertanika J. Trop. Agric. Sci. 2018, 41 (1), 19–40.

      4. Ahorsu R., Medina F., Constantí M. Significance and Challenges of Biomass as a Suitable Feedstock for Bioenergy and Biochemical Production: A Review. Energies. 2018, 11 (3366), 1–19. https://doi.org/10.3390/en11123366

      5. Jaiswal A. K., Williams G. A., Hassan S. S., Ravindran R. A Review on Bioconversion of Agro-Industrial Wastes to Industrially Important Enzymes.  Bioengineering. 2018, 5 (93), 1–20. https://doi.org/10.3390/bioengineering5040093

      6. DSTU ISO 1614:2003. Technical glycerol. Sampling and testing methods. General Information. (In Ukrainian).

      7. DSTU ISO 13906:2013. Animal feed. Methods of determining the content of kisotno-detergent fiber (KDF) and acid-detergent lignin. (In Ukrainian).

      8. DSTU ISO 2470:2005. Paper, cardboard and cellulose. Measurement of diffuse reflection coefficient in blue light. (In Ukrainian).

      9. DSTU EN 13041:2005. Miliananti soil and a medium of growth. Determination of physical properties. Density of dry weight, air volume, volume humidity, shrinkage and total pore space. (In Ukrainian).

      10. DSTU 4595:2006. Soy protein, technical conditions. (In Ukrainian).

      11. DSTU 3500-97. Paper and cardboard paper Tent conditions for change GOST 10700-84. (In Ukrainian).

      12. Shulga S. M., Tigunova O. A., Blume Y. B. Lignocellulose as an alternative source for obtaining of biobutanol.  Biotechnol. acta. 2013, 6 (2), 9–21. (In Ukrainian). https://doi.org/10.15407/biotech6.02.009

      13. Tigunova O., Shulga S. Using by mutant strains C. acetobutylicum ligno-cellulosic material as substrate. Microbiol. biotechnol. 2015, V. 3, P. 38–49. (In Ukrainian). https://doi.org/10.18524/2307-4663.2015.3(31).53656