Biotechnologia Acta

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"Biotechnologia Acta" V. 11, No 5, 2018
https://doi.org/10.15407/biotech11.05.065

Lactobacillus AS PRODUCERS OF EXTRACELLULAR TANNASE

L. Oriabinska1, O. Dziuba1, 2, O. Dugan1

Abstract

The aim of the work was to find strains of lactic acid bacteria capable to synthesize extracellular tannase enzyme − the key enzymehe hydrolyzing tannins which are  plant food constituent. One of the main product of tannins hydrolyzis is gallic acid- the compound with proven antioxidant and onco-protective properties. As a result of lactobacteria screening, two biocompatible strains of lactic acid, namely L. rhamnosus LB3 and L. delbrueckii subsp. delbrueckii with a high level of enzyme productivity, were selected. The maximum accumulation of tannase, corresponding to 0.031 ± 0.002 U/ml for L. rhamnosus LB3 and 0.03 ± 0.002 U/ml for L. delbrueckii subsp. delbrueckii, was observed after 48 h of cultivation. Both strains showed rapid growth and performance of tannase in MRS medium in the presence of glucose or lactose as a carbon source. It was shown that gallic acid, which was a necessary component of the medium as a target enzyme inducer, did not affect the accumulation of lactobacilli biomass. The selected strains are of interest as producers of a bicomponent probiotic with antioxidant properties and require further investigation.

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