Biotechnologia Acta

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"Biotechnologia Acta" V. 11, No 4, 2018
https://doi.org/10.15407/biotech11.04.068

DEVELOPMENT OF RECOMBINANT POSITIVE CONTROL FOR Francisella tularensis DETECTION  BY Q-PCR

O. B. Zlenko, A. P. Gerilovych

Abstract

The aim of the work was to construct and test the recombinant positive control for F. tularensis detection by a real-time polymerase chain reaction (qPCR). The molecular TA-cloning of pTZ57_F/R plasmid ligated with tul4 gene PCR product into DH5α E. coli. The minimal detection level in qPCR was one copy number per reaction. The obtained positive control was highly sensitive, specific and safe qPCR in the laboratory tularemia diagnostics.

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