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Home Archive 2008 № 2 MICROBIAL ?-AMYLASES: ISOLATION, PURIFICATION AND PRACTICAL USAGE L. D. Varbanets, K. V. Avdiyuk, N. V. Borzova
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ISSN 1995-5537

Scientific journal

БІОТЕХНОЛОГІЯ

V. 1, No. 2, 2008


“Biotechnology” journal Vol. 1, No.1, 2008
P. 116-122, Bibliography. 20, Ukrainian.
Universal Decimal classification: 579.852.11.222


MICROBIAL ?-AMYLASES: ISOLATION, PURIFICATION
AND PRACTICAL USAGE

L. D. Varbanets, K. V. Avdiyuk, N. V. Borzova

Institute of Microbiology and Virology of National Academy of Sciences of Ukraine, Kyiv

The ?-amylase family is the largest family of glycoside hydrolases, transferases and isomerases, comprising in clаn GH–H. The enzymes of this family, except of ?-1,4-, ?-1,6-bonds, are able to hydrolyse ?-1,-1, ?-1,2-, ?-1,3-, ?-1,5-glycosidic bonds, contain 4 conserved aminoacid residues (Asp204, Asp206, Glu230 и Asp297) and have (?/?)8 or TIM barrel catalytic domain. The questions conserning methods of isolation and purification of ?-amylases, which include the precipitation of organic solvents and neutral salts, dialysis, ultrafiltration, gelfiltration, chromatography, electrophoresis are discussed. The current data on possibility of ?-amylases from different taxonomic groups of microorganisms, in particular, bacteria and micromycetes are given. Physicochemical properties of ?-amylases, its pH- and thermooptimum, рН- and thermostability, molecular masses, influence of metal ions and different chemical reagents are discussed in detail. It’s shown the possibility of ?-amylases usage in different branches of industry — food, detergent, paper, textile, pharmaceutical.


Кey words: microbial ?-amylases, physicochemical properties, practical usage.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008