Biotechnologia Acta


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Home Archive 2018 № 5 AMPHIBIAN SKIN SECRETIONS: A POTENTIAL SOURCE OF PROTEOLYTIC ENZYMES Nikolaieva, Yu. Dudkina, D. Oliinyk, O. Oskyrko, O. Marushchak, T. Halenova, O. Savchuk
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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

"Biotechnologia Acta" V. 11, No 5, 2018
Р. 42-48, Bibliography 22, English
Universal Decimal Classification:  615.919+577.152.34


I. Nikolaieva1, Yu. Dudkina1, D. Oliinyk1, O. Oskyrko1, O. Marushchak2, T. Halenova1, O. Savchuk1

1Taras Shevchenko National University of Kyiv, Ukraine
2Schmalhausen Institute of Zoology of the National Academy of Sciences of Ukraine, Kyiv

The aim of the work was to study the protein content and proteolytic activity of the skin glands secretions of 10 the most common types of amphibians on the territory of Ukraine such as B. bombina, B. variegata, B. bufo, B. viridis, R. temporaria, P. ridibundus, P. esculentus, P. fuscus, S. salamandra, as well as the hybrid of B. bombina and B. variegata species. It was shown that the skin secretions of the studied amphibians contained a wide range of proteins with a molecular weight in the range from 8 to 150 kDa. By enzyme electrophoresis using gelatin, fibrinogen and collagen as substrates, it was found that they contained proteinases that differ in substrate specificity. It was revealed that the skin glands secretions of B. bombina, S. salamander species, as well as the hybrid of B. bombina and B. variegata species were characterized by he increased protein content with gelatinase and collagen activity.

Key words: amphibians, skin gland secretions, proteolytic activity.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2018

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