Biotechnologia Acta

...

  • Increase font size
  • Default font size
  • Decrease font size
Home Archive 2018 № 4 APPLICATION OF GLUTAMATE-SENSITIVE BIOSENSOR FOR ANALYSIS OF FOODSTUFF Kucherenko D. Yu.1, Kucherenko I. S, Soldatkin O. O., Soldatkin A. P.
Print PDF

ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

"Biotechnologia Acta" V. 11, No 4, 2018
https://doi.org/10.15407/biotech11.04.057
Р. 57-67, Bibliography 27, English
Universal Decimal Classification: 543.06 + 577.15 + 543.553

APPLICATION OF GLUTAMATE-SENSITIVE BIOSENSOR FOR ANALYSIS OF FOODSTUFF

Kucherenko D. Yu.1, Kucherenko I. S.2, Soldatkin O. O.1, 2, Soldatkin A. P.1, 2

1Institute of High Technologies, Taras Shevchenko Kyiv National University
2Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, NAS of Ukraine

The aim of the work were the optimization of an amperometric glutamate-sensitive biosensor and its utilization for the determination of the glutamate concentrations in food samples. Amperometric method of measurements was used. The biosensor was based on immobilized glutamate oxidase and platinum disc electrode. The biosensor was connected to the working cell with auxiliary (platinum wire) and reference (Ag/AgCl) electrodes. The biosensor exhibited high sensitivity to glutamate, duration of one analysis was about 5 min. An influence of the ionic strength, pH, and buffer capacity on the biosensor operation was investigated. The sensitivity of biosensor to various possible interfering substances, including amino acids, was studied; high selectivity to glutamate was shown. The reproducibility of analysis of food samples and an impact of sample dilution was evaluated. Glutamate concentrations in different sauces and seasonings were measured by the developed biosensor; the results correlated well with those obtained by the spectrophotometric method (R2 = 0,988). Thus, the amperometric biosensor for glutamate determination was successfully optimized and used for measurement of glutamate concentrations in sauces and seasonings.

Key words: amperometric biosensor, glutamate oxidase, poly(phenylenediamine), glutamate, food samples.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2018

 

Additional menu

Site search

Site navigation

Home Archive 2018 № 4 APPLICATION OF GLUTAMATE-SENSITIVE BIOSENSOR FOR ANALYSIS OF FOODSTUFF Kucherenko D. Yu.1, Kucherenko I. S, Soldatkin O. O., Soldatkin A. P.

Invitation to cooperation

Dear colleagues, we invite you to publish your articles in our journal.
© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2008.
All rights are reserved. Complete or partial reprint of the journal is possible only with the written permission of the publisher.
E-mail
for information: biotech@biochem.kiev.ua.