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Home Archive 2018 № 3 EXTRACTS OF GANODERMA SPECIES CHARACTERIZATION: THEIR ANTIOXIDANT ACTIVITY AND FINGERPRINTS V. Raks, M. Öztürk, O. Vasylchenko, M. Raks
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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

"Biotechnologia Acta" V. 11, No 3, 2018
https://doi.org/10.15407/biotech11.03.069
Р. 69-77, Bibliography 13, English
Universal Decimal Classification:  582.284:543.061

GANODERMA SPECIES EXTRACTS: ANTIOXIDANT ACTIVITY AND CHROMATOGRAPHY

V. Raks 1, 2, M. Öztürk 2, O. Vasylchenko 3, M. Raks 4

1 Taras Shevchenko National University of Kyiv, Ukraine
2 Mugla Sitki Kocman University, Turkey
3 National Aviation University, Kyiv, Ukraine
4 National University of Food Technologies, Kyiv, Ukraine

Research aimed to isolate biologically active compounds from mushrooms fruiting bodies of Ganoderma lucidum, Ganoderma adspersum and Ganoderma applanatum and estimate their antioxidant activities. Variety of sample preparation techniques were used to isolate biologically active compounds: solid-liquid, ultrasonic extraction, Soxhlet extractor extractions. Antioxidant properties were estimated with spectrophotometricaly measuring free radical scavenging activity. High performance liquid chromatography was applied to get fingerprints of isolated extracts. In β-carotene-linoleic acid assay, methanol extracts demonstrated mainly the best antioxidant activities: optimum values of half maximal inhibitory concentration (IC50) − for G. applanatum and G. adspersum were 8.25 ± 0.88 µg/ml and 1.70 ± 1.13 µg/ml respectively. However, petroleum ether and chloroform extracts of G. lucidum demonstrated the highler antioxidant activity with an IC50 about 33.66 ± 3.69 µg/ml. Chromatograms of dry components of acetone and methanol extracts of G. lucidum were recorded. The main outcome of such chromatograms is the possibility to compare presence active components in various mushrooms species without usage of expensive standards.

Key words: Ganoderma species mushrooms, antioxidant activity, high performance liquid chromatography.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2018

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