Biotechnologia Acta

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Home Archive 2018 № 2 Wastewater components effect on metachromasia reaction of volutin granules in vitro Chudina T., Skoryk M., Legach Е.
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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

"Biotechnologia Acta" V. 11, No 2, 2018
https://doi.org/10.15407/biotech11.02.02
Р. 23-29, Bibliography 12, English
Universal Decimal Classification: 004:591.5:612:616-006

SYNTHESIS AND CHARACTERIZATION OF POLY(D,L-LACTIC-CO-GLYCOLIC)ACID MICROPARTICLES LOADED BY DIPHTHERIA TOXOID

Chudina T. О.1, 2, Skoryk M. А.3, Legach E. I.4

1Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, Kyiv
2 ESC ‘Institute of Biology and Medicine’ Taras Shevchenko National University of Kyiv, Ukraine
3NanoMedTech LLC, Kiev, Ukraine
4 Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

The aim of the study was to synthesize poly(D,L-lactide-co-glycolide) particles — PLGA and obtain their complexes with a non-toxic diphtheria toxin recombinant fragment subunit B fused with enhanced green fluorescent protein EGFP-SubB; to characterize the main physical and chemical properties of the resulting complexes.

Two types of the diptheria toxoid loaded microparticles were obtained: particles with immobilized diphtheria toxoid (PLGA 1) and particles with encapsulated (PLGA) diphtheria toxoid (PLGA 2). Micropartcles obtaines were characterized for antigen loading, particle size, polydispersity index and in vitro antigen release.

Key words: diphtheria toxoid, poly (lactic-co-glycolic-acid) microparticles.

Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2018