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Home Archive 2008 № 2 THE TECHNOLOGICAL PARAMETERS OF CULTIVATION CELLS WHICH ARE PRODUCERS OF TYPE I INTERFERONS Yu. M. Penchuk, O. V. Karpov, V. M. Povodzinsky, S. V. Veriovka, Z. R. Ul’berg
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ISSN 1995-5537

Scientific journal

БІОТЕХНОЛОГІЯ

V. 1, No. 2, 2008


“Biotechnology” journal Vol. 1, No. 2, 2008
Р. 69-74, Bibliography 7, Ukrainian.
Universal Decimal classification 578.245:577.1113.7:57.086.833

 

National Universyti of Food Technologies, Kyiv
Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv
Ovcharenko Institute of Biocolloid Chemisrty
of Nationale Academy of Sciences of Ukraine, Kyiv

 

The authors investigated the main technological parameters of cultivation of interferon type I (?/?-IFN) producing cells in home-made experimental devise; the yeast RNA-tilorone hydrochlorid molecular complex immobilized on Sferon-300 beads (IMMC) was used as an IFN inducer. The experiments were made using human blood mononuclear cells (suspension culture) and established testicular porcine cells (monolayer culture). The authors determined the optimal producing cell quantity/IMMC beads ratios as well as the optimal cultural medium stirring velocity for each culture. There are conclusions concerning the importance of parameters mention above using bioreactors to obtain IFN preparations during large-scale cultivation using the IMMC.

Кey words: interferon, inductor, immobilization, cpheron, cell culture, roller cultivation, mononuclears.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008

 

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Home Archive 2008 № 2 THE TECHNOLOGICAL PARAMETERS OF CULTIVATION CELLS WHICH ARE PRODUCERS OF TYPE I INTERFERONS Yu. M. Penchuk, O. V. Karpov, V. M. Povodzinsky, S. V. Veriovka, Z. R. Ul’berg

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