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Home Archive 2018 № 1 USING OF LOWLAND PEAT SUBSTRATE FOR CULTIVATION OF PICHIA ANOMALA IMB Y-5067 AND RHODOTORULA GRACILIS IMB Y-5075 YEAST STRAINS T. S. Kalandyrets, V. O. Krasinko, H. S. Andriiash, N. Ye. Beiko, О. О. Tigunova, S. M. Shulga
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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

"Biotechnologia Acta" V. 11, No 1, 2018
https://doi.org/10.15407/biotech11.01.064
Р. 64-69, Bibliography 13, English
Universal Decimal Classification: 577.115:663.12

USING OF LOWLAND PEAT SUBSTRATE FOR CULTIVATION OF PICHIA ANOMALA IMB Y-5067 AND RHODOTORULA GRACILIS IMB Y-5075 YEAST STRAINS

T. S. Kalandyrets 1, V. O. Krasinko 1, H. S. Andriiash 2, N. Ye. Beiko 2,
О. О. Tigunova 2, S. M. Shulga 2

1 National University of Food Technologies, Kyiv, Ukraine
2 SE “Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine”, Kyiv

The aim of the research was to study the accumulation of yeast lipids in the process of cultivation of strains P. anomala IMB Y-5067 and R. gracilis IMB Y-5075 with the use of peat as growing substrate. The object of researches was strains P. anomala IMB Y-5067 and R. gracilis IMB Y-5075 from “Collection of strains of microorganisms and plant lines for food and agricultural biotechnology” of SE “Institute of Food Biotechnology and Genomics of the National Academy of Sciences of Ukraine”. As a raw material, lowland peat which was preliminarily processed with the help of cavitation or explosive autohydrolysis was used. The accumulation of lipids over the course of cultivation P. anomala IMB Y-5067 and R. gracilis IMB Y-5075 on non-food substrate ‒ lowland peat was shown. The effect of pulp explosive autohydrolysis and pulp cavitation processing on biomass accumulation and lipids synthesis by strains P. аnomala IMB Y-5067 and R. gracilis IMB Y-5075 was researched. It was found that the maximum lipids accumulation by strains P. аnomala IMB Y-5067 (9.7 g/dm3) and R. gracilis IMB Y-5075 (8.9 g/dm3) was over the course of cavitation processing of peat pulp and additional application of salts and yeast extract into cultivation environment.

Key words: peat, Pichia anomala, Rhodotorula gracilis, yeast, cultivation, lipids.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2017

  • References
    • 1. Chuchuy V. P., Uminskiy S. M., Inyutin S. V. Alternative Energy Sources. Odesa: TES. 2015, 494 p. (Іn Ukrainian).

      2. Kuhar V. P. Bioresources — potential raw material for industrial organic synthesis. Kataliz i neftekhimiya. 2007, 15, 1–15. (Іn Russian).

      3. Chuprina L. A. Organic waste recycling: Ukrainian technologies. Shlyahi rozvitku ukrayinskoyi nauki. 2016, V. 1, P. 115–117. (Іn Ukrainian).

      4. Tigunova O. O., Beiko N. E., Kamenskyu D. S., Tkachenko T. V., Yevdokymenko V. O., Kashkovskiy V. I., Shulga S. M. Lignocellulosic biomass after explosive autohydrolysis as substrate for butanol obtaining. Biotechnol. acta. 2016, 9 (4), 28‒34. https://doi.org/10.15407/biotech9.04.028

      5. Saranchuk V. I., Ilyashov M. O., Oshovskii V. V., Biiletskii V. S. Fundamentals of Chemistry and Physics of Combustible Minerals. Donetsk: Shidnyi vydavnychyi dim. 2008, 640p. (Іn Ukrainian).

      6. Baümler E. R. Solvent extraction: kinetic study of major and minor compounds. JAOCS. 2010, V. 87, P. 1489–1495.

      7. Lakin G. F. Biometrics. Moskva: Vyisshaya shkola. 1990, 300 p. (Іn Russian). Experimental articles 69.

      8. Veshnyakov V. A., Habarov Yu. G., Kamaki na N. D. Comparison of methods for the deter mi na tion of reducing substances: the Bertrand method, ebulliostatic and photometric methods. Khimiya rastitelnogo syrya. 2008, 4, 47–50. (Іn Russian).

      9. Gerasimov Yu. Yu., Hlyustov V. K. Mathematical methods and models in computer calculations. Moskva: MGUL. 2001, 260 p.

      10. Verhoeven Jos T. A., Setter Tim L. Agricultural use of wetlands: opportunities and limitations. Ann Bot. 2010, 105 (1), 155–163. dhttps://doi.org/10.1093/aob/mcp172

      11. Tkachenko A. F., Tigunova E. A., Shulga S. M. Microbial lipids — an alternative source of raw materials for biofuels. Mikrobiologiya i biotekhnologiya. 2012, V. 3, P. 17–33. (Іn Russian).

      12. Tkachenko A. F., Tigunova E. A., Shulga S. M. Lipids of microorganisms as a source of biofuel. Tsitologiya i genetika. 2013, 6 (47), 22–29. (Іn Russian).

      13. Shulga S. M., Tkachenko A. F., Beyko N. E., Homenko A. I., Andriyash A. S. Biosynthesis of lipids by yeast Rhodotorula gracilis. “Biotekhnolohiia”. 2010, 3 (3), 58–65. (Іn Russian).


 

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Home Archive 2018 № 1 USING OF LOWLAND PEAT SUBSTRATE FOR CULTIVATION OF PICHIA ANOMALA IMB Y-5067 AND RHODOTORULA GRACILIS IMB Y-5075 YEAST STRAINS T. S. Kalandyrets, V. O. Krasinko, H. S. Andriiash, N. Ye. Beiko, О. О. Tigunova, S. M. Shulga

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