Biotechnologia Acta

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Home Archive 2017 № 6 INFLUENCE OF CULTURE MEDIUM ON THE OYSTER MUSHROOMS STRAINS MYCELIUM GROWTH I. V. Pereima, T. V. Ivanova
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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

"Biotechnologia Acta" V. 10, No 6, 2017
https://doi.org/10.15407/biotech10.06.045
Р.  45-52, Bibliography  6, English
Universal Decimal Classification: 579.61

STIMULATION OF GROWTH OF SPECIES OF THE FUNGUS OF THE GENUS Pleurotus (Fr.) P. Kumm. AT A GLUCOSE NUTRITION

I. V. Pereima, T. V. Ivanova

National University of Life and Environmental Science of Ukraine, Kiyv

The purpose of this study was to evaluate the optimal nutritional conditions for macromycetes micelles of the Pleurotaceae family. The factors influencing mycelium growth on the example of two species of Pleurotus were investigated.
To determine the effects of nutrient components five variants of agar medium were used, containing:potato, sweet potato, yam, oak bark and malt extractswith the addition of peptone and glucose. The pure culture of Pleurotus species, in the form of agarics blocks, was placed on the nutrient media. Hyphae germination was observed on the eighth day of incubation.

The temperature influence was evaluated in the range from 16 to 36 °C. The maximum growth of mycelium of both species was observed at a temperature of 28 °С.

It was determined that nutrient media containing potato, sweet potato, yam and malt extracts with the addition of glucose and peptone were the most favorable for growth of mycelium of oyster mushroom. They all had mandatory presence of a source of carbohydrates — glucose. At the same time, the media containing extracts of oak, yam, and partially of potato extract, were optimal for the growth of maple oyster. According to the experiment, the mycelial growth efficiency of the maple oyster was much higherin comparison to the oyster mushroom 1.

Key words: Pleurotus, nutrient media, mycelium.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2017

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