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INCREASING OF THE EXPRESSION OF RECOMBINANT scFv-ANTIBODIES EFFICIENCY O.V. Galkin, T.O. Chudina, A.A. Siromolot, O.S. Oliynyk

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INCREASING OF THE EXPRESSION OF RECOMBINANT scFv-ANTIBODIES EFFICIENCY

O.V. Galkin, T.O. Chudina, A.A. Siromolot, O.S. Oliynyk

"Biotechnologia Acta" V. 10, No 5, 2017
https://doi.org/10.15407/biotech10.05.019

Abstract
Obtaining single-chain variable fragments (scFv) of recombinant antibodies in E. coli cells is often associated with numerous problems causing low yields or inactive conformation of the product.

The aim of this work was to study the influence of staphylococcal protein A fragment fused with scFv antibodies (SpA-tag) on the efficiency of expression of  final product. Examination of scFv antibodies of different origin and specificity  has shown that in similar expression systems fused scFv is synthesized in much higher quantities than free scFv. Furthermore, the scFv antibodies in fused form retained their antigen-binding properties and the SpA fragment the ability to bind other immunoglobulins.

Thus, the proposed strategy can be considered effective in improving the efficiency of scFv-antibodies production in E. coli cells.

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