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BIOBUTANOL ACCUMULATION USING ALTERNATIVE SUBSTRATES BY CULTIVATION OF Clostridium acetobutylicum STRAINS О. О. Тigunova, H. S. Аndriiash, N. Ye. Beiko, І. V. Melnyk, S. М. Shulga

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BIOBUTANOL ACCUMULATION USING ALTERNATIVE SUBSTRATES BY CULTIVATION OF Clostridium acetobutylicum STRAINS
О. О. Тigunova, H. S. Аndriiash, N. Ye. Beiko, І. V. Melnyk, S. М. Shulga

"Biotechnologia Acta" V. 10, No 5, 2017
https://doi.org/10.15407/biotech10.05.036

Абстракт
The aim of the research was to investigate the accumulation of biobutanol by strains C. acetobutylicum using alternative substrates (Jerusalem artichoke juice, technical glycerol, crude glycerol, shredded biomass of soy, rape, wheat and switchgrass). In order to increase the accumulation of butanol in the process of cultivation, the cells of C.acetobutylicum were statically immobilized on carriers (belting strips, ferrite rings and Raschig rings) by the method of adsorption immobilization. The cells were precipitated by centrifugation, the supernatant was distilled and then fermentation products were determined. Gas chromatography was used to determine the presence of solvents in the culture fluid. The biggest accumulation of  butanol (2 g/dm3) was at concentration of crude glycerol 16 g/dm3 in the medium, and complete inhibition of culture development ‒ at concentration of 25 g/dm3. The accumulation of butanol by the strain Clostridium acetobutylicum IMB B-7407 using fill and draw method depended on the amount of sequestered and infused medium. Immobilization of the culture using the Raschig rings allowed increasing the bioconversion to butanol twice. So it is shown the possibility to use non-traditional substrates for the production of biobutanol. Immobilization of C.acetobutylicum culture cells on carriers increased the accumulation of butanol. The use of Raschig rings, as carriers for immobilization, allowed increasing the accumulation of  butanol twice.

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