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Home Archive 2017 № 2 IDENTIFICATION OF PSY1 GENE ALLELES RESPONSIBLE FOR CAROTENOID ACCUMULATION IN WHEAT GRAINS O. V. Stepanenko, A. І. Stepanenko, Ye. V. Kuzminskiy, B. V. Morgun
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"Biotechnologia Acta" V. 10, No 2, 2017
DOI: 10.15407/biotech10.02.057
Р.  57-66, Bibliography 21, English
Universal Decimal Classification: 577.21+ 633.11

IDENTIFICATION OF Psy1 GENE ALLELES RESPONSIBLE FOR CAROTENOID ACCUMULATION IN WHEAT GRAINS

O. V. Stepanenko 1, 2, A. І. Stepanenko 1, Ye. V. Kuzminskiy 2, B. V. Morgun 1

1 Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine, Kyiv  
2 National Technical University of Ukraine «Igor Sikorsky Kyiv Polytechnic Institute», Kyiv

The aim of the research was to select and optimize marker systems for identification of Psy1 genes alleles, which are responsible for different levels of carotenoid pigments accumulation in wheat grains, as well as to screen varieties for the selection of valuable genotypes. 162 wheat samples were analyzed by the polymerase chain reaction method. Among them, varieties and lines with different allelic states of Psy-A1 and Psy-B1 genes were identified. The Psy-D1 gene did not show any polymorphism. As a result, samples with valuable alleles of Psy1 genes, which potentially contain increased carotenoids content in grains, were selected.

Key words: Psy1 genes, carotenoids, molecular markers, Triticum aestivum.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2017

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