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Home Archive 2016 № 6 mRNA expression, CTS, LONP1/PRSS15, IRE1 inhibition, glucose deprivation, U87 glioma cells.
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"Biotechnologia Acta" V. 9, No 6, 2016
https://doi.org/10.15407/biotech9.06.016
Р. 16-27, Bibliography 46, English
Universal Decimal Classification:  577.112:616

GLUCOSE DEPRIVATION AFFECTS THE EXPRESSION OF LONP1 AND CATHEPSINS IN IRE1 KNOCKDOWN U87 GLIOMA CELLS

O. H. Minchenko 1, O.V. Halkin 1, O. O. Riabovol 1, D. O. Minchenko 1, 2, A. Y. Kuznetsova 1, O. O. Ratushna 1

1 Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, Kyiv
2 Bohomolets National Medical University, Kyiv, Ukraine

To study the effect of glucose deprivation on the expression of genes encoding for LONP1/PRSS15 and cathepsins in U87 glioma cells in relation to inhibition of inositol requiring enzyme-1 (IRE1) was the aim of the research. It was shown that glucose deprivation up-regulated the expression of CTSA, CTSB, CTSD, CTSK, CTSL, CTSO, and LONP1 genes and did not change the expression of CTSC, CTSF, and CTSS genes in control glioma cells (transfected by empty vector). Inhibition of ІRE1 signaling enzyme function in U87 glioma cells modified effect of glucose deprivation on the expression of most studied genes: removed the effect of glucose deprivation on CTSA and CTSO genes, introduces on CTSC and CTSS genes, reduced – on CTSK gene, and enhanced – on CTSL gene. Therefore, glucose deprivation affect the expression level of most studied genes in relation to the functional activity of IRE1 enzyme, a central mediator of endoplasmic reticulum stress, which control cell proliferation and tumor growth.

Ключові слова: mRNA expression, CTS, LONP1/PRSS15, IRE1 inhibition, glucose deprivation, U87 glioma cells.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2016