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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

 4 2015

"Biotechnologia Acta" V. 8, No 4, 2015
Р. 98-107, Bibliography 19, Ukrainian
Universal Decimal Classification: 577(112+25+354.9)+57(085.2+086.2)
https://doi.org/10.15407/biotech8.04.098

 OBTAINING OF RECOMBINANT ANALOGUE OF HUMAN SHB-EGF USING OF TRANSPOSON-TRANSPOSASE GENETIC SYSTEM

 N. V. Korotkevych, A. Yu. Labyntsev, D. V. Kolybo, S. V. Komisarenko

Palladin Institute of Biochemistry of the National Academy of Scienses of Ukraine, Kyiv

The goal of work was obtaining of recombinant soluble form of heparinbinding EGF-like growth factor sHB-EGF in eukaryotic cells based on transposon genetic system. We used methods of recombinant DNA and obtaining of eukaryotic cells for  producing of recombinant proteins. We have obtained stable eukaryotic producer of recombinant analogue of soluble form of human heparinbinding EGF-like growth factor sHB-EGF based on HEK293 cells. We used transposon based non-viral expression system for sHBEGF expression. Moreover, studies in mice have shown the possibility of using sHB-EGF expressing genetic constructs for in vivo gene therapy of various pathological conditions.

Key words: heparin-binding EGF-like growth factor HB-EGF; transposase; recombinant proteins; growth factors.

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2015