Biotechnologia Acta

...

  • Increase font size
  • Default font size
  • Decrease font size
Home Archive 2016 № 4 LIGNOCELLULOSIC FEEDSTOCKS AFTER EXPLOSIVE AUTOHYDROLYSIS AS SUBSTRATE TO BUTANOL OBTAINING O. O. Tigunova, N. E. Beiko, D. S. Kamenskykh, T. V. Tkachenko, V. O. Yevdokymenko, V. I. Kashkovskyi, S. M. Shulga
Print PDF

ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

"Biotechnologia Acta" V. 9, No 4, 2016
https://doi.org/10.15407/biotech9.04.028
Р. 28-34, Bibliography 13, English
Universal Decimal Classification: 547.917:547.458.1;579.222

LIGNOCELLULOSIC BIOMASS AFTER EXPLOSIVE AUTOHYDROLYSIS AS SUBSTRATE TO BUTANOL OBTAINING

O. O. Tigunova 1, N. E. Beiko1, D. S. Kamenskykh 2, T. V. Tkachenko 2, V. O. Yevdokymenko 2, V. I. Kashkovskyi 2, S. M. Shulga 1

1Institute of Food Biotechnology and Genomics NAS of Ukraine, Kyiv,
2Institute of Bioorganic Chemistry and Petrochemistry, NAS of Ukraine, Kyiv

The aim of the work was investigation of the effect of the explosive autohydrolysis on lignocellulosic biomass (saving, switchgrass biomass) for consequent use as a substrate to produce biofuels such as butanol. Butanol-producing strains, switchgrass Panicum virgatum L. biomass and its components after autohydrolysis were used in study. The thermobaric pressure pretreatment of lignocellulosic biomass was carried out using specially designed equipment. The effect of explosive autohydrolysis on lignocellulosic biomass for further use in producing biofuels using microbial conversion was studied. Components of lignocellulosic biomass were fractionated after undergoing thermobaric treatment. The possibility of using different raw material components after using explosive autohydrolysis processing to produce biobutanol was found. Products of switchgrass biomass autohydrolysis were shown to need further purification before fermentation from furfural formed by thermobaric pretreatment and inhibiting the growth of microorganisms. The ability of strains of the genus Clostridium to use cellulose as a substrate for fermentation was proved. It was found that using explosive autohydrolysis pretreatment to savings allowed boosting the butanol accumulation by 2 times.

Key words: explosive autohydrolysis, butanol, lignocellulosic raw materials, bio fuel

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2016

  • References
      1. Shulga S. M., Tigunova O. O., Blume Y. B. Lignocellulose as an alternative source for obtaining of butanol. Biotechnol. аcta. 2013, 6 (2), 9–21. (In Ukrainian).
      2. Kamenskykh D. S., Tkachenko T. V., Yevdokymenko V. O., Kashkovskyi V. I. Explosive autohydrolysis of pentosan-containing raw material. Catalysis and Petrochemicals. 2015, V. 24, P. 90–95. (In Ukrainian).
      3. 3. Tіgunova O. O., Shulga S. M. Using by mutant strains C. acetobutylicum ligno-cellulosic material as a substrate. Microbiol. and biotechnol. 2015, V. 3, P. 35–44.
      4. 4. GOST 13496-92. Mixed feeds, mixed fodder raw materials. Methods for determination of moisture. 1.1.93. (In Russian).
      5. 5. GOST 26226-95. Feed, mixed feeds, feed raw materials. Methods for the determination of crude ash. 10.12.95. (In Russian).
      6. 6. GOST 26177-84. Feed, mixed feeds. Method for the determination of lignin. 12.1.99 (In Russian).
      7. 7. GOST 26176-91. Feed, mixed feeds. Methods for determination of readily soluble and carbohydrates. 1.1.93. (In Russian).
      8. 8. GOST 6841-77. Cellulose. Method for determination of tar and fats. 01.07.98. (In Russian).
      9. Obolenskaia A. V., Elnytskaia Z. P., Leonovych A. A. Laboratory work on the chemistry of wood and cellulose. Moskva: Chemistry. 1977, 232 p. (In Russian).
      10. Tigunova O. O., Shulga S. M. New producer strains of biobutanol. I. Isolation and identification. Biotechnol. acta. 2013, 6 (1), 97–104. (In Ukrainian).
      11. Tigunova O. O., Shulga S. M. New producer strains of biobutanol. ІІI. Methods increased accumulation of butanol from switchgrass Panicum virgatum L. biomass. Biotechnol. acta. 2015, 8 (4), 65–68. (In Ukrainian).
      12. Trofymova N. N., Babkyn V. A., Chemerys M. M. Kataliziruemyj parovzryvnoj gidroliz celloligninovogo ostatka drevesiny listvennicy. Himija rastitel'nogo syr'ja. 2002, V. 2, P. 53–56. (In Russian).
      13. Kumar Y. S., Negi J. S. Upadhyaya Studies on characterization of corn cob based nanoparticles. Adv. Mat. Lett. 2010, 1 (3), 246–253.



 

Additional menu

Site search

Site navigation

Home Archive 2016 № 4 LIGNOCELLULOSIC FEEDSTOCKS AFTER EXPLOSIVE AUTOHYDROLYSIS AS SUBSTRATE TO BUTANOL OBTAINING O. O. Tigunova, N. E. Beiko, D. S. Kamenskykh, T. V. Tkachenko, V. O. Yevdokymenko, V. I. Kashkovskyi, S. M. Shulga

Invitation to cooperation

Dear colleagues, we invite you to publish your articles in our journal.
© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2008.
All rights are reserved. Complete or partial reprint of the journal is possible only with the written permission of the publisher.
E-mail
for information: biotech@biochem.kiev.ua.