Biotechnologia Acta

...

  • Increase font size
  • Default font size
  • Decrease font size
Home Archive 2016 № 4 OPTIMIZATION OF Chlorella vulgaris BEIJ. CULTIVATION IN A BIOREACTOR OF CONTINUOUS ACTION O. I. Bodnar, N. V. Burega, A. O. Palchyk, H. B. Viniarska, V. V. Grubinko
Print PDF

ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

"Biotechnologia Acta" V. 9, No 4, 2016
https://doi.org/10.15407/biotech9.04.042
Р. 42-49, Bibliography 23, English
Universal Decimal Classification: 561.263+57.086.83

OPTIMIZATION OF Chlorella vulgaris BEIJ. CULTIVATION IN A BIOREACTOR OF CONTINUOUS ACTION

O. I. Bodnar, N. V. Burega, A. O. Palchyk, H. B. Viniarska, V. V. Grubinko

Volodymyr Gnatiuk Ternopil National Pedagogical University, Ukraine

The aim of the research was the development and testing of bioreactor for intensive cultivation of algae Chlorella vulgaris Beij. with fully controlled conditions within the operating parameters according to the selected evaluation criteria of the cultivation process. To check the functioning efficiency of the decigned photobioreactor, the growth of Chlorella vulgaris Beij. (CHLOROPHYTA) in Fitzgerald’s medium modified by Zender and Gorham (No 11) under the artificial illumination with daylight electric lamps (intensity of 2 500 Lx) for 16 hours a day at 22–25 ºС was studed. It was found that at stabilization of culture conditions the maximum value of culture density was observed at the 18th day of cultivation. In this moment, the amount of cells reached 269.2 ± 3.0·109 cells/l, while cells amount in stationary phase was within 110.1 ± 4.9·109 cells/l. This makes it possible the continuous chlorella cultivation with an average productivity in stationary mode of about 110 ± 4 mg/l of dry mass with protein content about 60 mg, carbohydrates  about 35 and lipids about 12 mg of dry mass/l. Sunlight and activators of biosynthesis of organic substances of individual classes allow changeing the ratio of proteins, carbohydrates and lipids that is prospective for further research.

Key words: chlorella, photobioreactor, continuous cultivation.

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2016

  • References
    • 1.  Handbook of microalgal culture. N. Y: Wiley Blackwell, 2003, 584 p.

      2.  Campbell M. N. Biodiesel: Algae as a Renewable Source for Liquid Fuel. Guelph Engin. J. 2008, V. 1, P. 2–7.

      3.  Abd El Baky H. H., El-Baroty G. S. Healthy Benefit of Microalgal Bioactive Substances. J. Aquat. Sci. 2013, 1 (1), 11–23.

      4.  Herrero M., Cifuentes A., Ibanez E. Supercritical fluid extraction of functional ingredients from different natural sources: Plants, food-by-products, algae and microalgae. A review. Food Chem. 2006, N 98, P. 136–148.

      5.  Kim Y. J., Kwon S., Kim M. K. Effect of Chlorella vulgaris intake on cadmium detoxification in rats fed cadmium. Nutr. Res. Pract. 2009, 3 (2), 89–94.

      6.  Lee H. S., Park H. J., Kim M. K. Effect of Chlorella vulgaris on lipid metabolism in Wistar rats fed high fat diet. Nutr. Res. Pract. 2008, 2 (4), 204–210.

      7.  Vinyarska H. B., Lykhatskiy P. H., Bodnar O. I., Fira L. S., Grubinko V. V. Effects of selenium-zinc-lipid substances from Chlorella vulgaris Biej. on oxidative and tnergy status of rats. Medychna i clinichna khimiia. 2015, 17 (4), 10–17. (In Ukrainian).

      8.  Lukashiv O. Ya., Bodnar O. I., Vasilenko O. V., Grubinko V. V. The effect of selenium-chrome-lipid substance from Chlorella vulgaris Biej. On energy metabolism in rats. Proceedings of the II International Scientific and Practical Conference “Topical researches of the World Science”. Dubai, UAE, June 29–30, 2016. 2016, 7 (11), 17–21.

      9.  Bogdanov N. I., Kunitsin M. V. The method of cultivation of microalgae on the basis of the strain Chlorella vulgaris IFR № С-111. Russian Federation Patent 2176667. December 10, 2001. (In Russian).

      10.  Kim S., Park J. E., Cho Y. B., and Hwang S. J. Growth rate, organic carbon and nutrient removal rates of Chlorella sorokiniana in autotrophic, heterotrophic and mixotrophic condi-tions. Bioresour. Technol. 2013, 144 (1), 8–13.

      11.  Sidorov Yu. I. Photobioreactors. Biotekhnolohiia. 2010, 3 (5), 19–30. (In Ukrainian).

      12.  Kravchenko I. P., Karpenko V. I., Didkivs’ka H. H. Laboratory photobioreactor. Ukraine Patent 102777. November 25, 2015. (In Ukrainian).

      13.  Chernov P. Ya. Photobioreactor for cultivation of microalgae. Ukraine Patent 93282. January 25, 2011. (In Ukrainian).

      14.  Schamansky S. Yo. Installation for bioconversion of solar energy continuous operation. Naukoyemki technolohii. 2015, 26 (2), 115–119. (In Ukrainian).

      15.  Methods physiological and biochemical studies of algae in hydrobiological practice. Ed. by Topachevsky A. V. Kyiv: Naukova Dumka. 1975, 247 p. (In Russian).

      16.  Topachevsky A. V., Masyuk N. P. Freshwater algae of the Ukrainian SSR. – Kyiv: Naukova Dumka.1984, 336 p. (In Russian).

      17.  Lowry O. H., Rosenbroug N. I., Farr A. L., Randall R. I. Protein measurement with the folin phenol reagent. J. Biol. Chem. 1951, 193 (1), 265–275.

      18.  Filippovych Yu. B., Egorova T. A., Sevast’yanova H. A. Practicum on General biochemistry. Moskva: Prosveshcheniye. 1975, 318 p. (In Russian).

      19.  Hokin L. E., Hexum T. D. Studies on the characterization of the sodium-potassium transport adenosine triphosphatase: IX. On the role of phospholipids in the enzyme Studies on the characterization of the sodium-potassium transport adenosinetriphosphatase: XIII. On the organization and role of phospholipids in the purified enzyme. Arch. Biochem. Biophys. 1992, 151 (2), 58–61.

      20.  Lebedeva L. P., Dzschokebaeva S. A. Optimizationof the growth processes of chlorella and spirulina and the use of pure extracts as biologically active additives to feed fish. Vestnik KazNU. Serya ekologicheskaya. 2012, 1 (33), 96–99. (In Russian).

      21.  Gandzyura V. P., Grubinko V. V. Concept of harmfulness in ecology. Kyiv-Ternopil: Publishing house of TNPU after name V. Hnatiuk. 2008, 144 p. (In Ukrainian).

      22.  Gorda А. I., Grubinko V. V. Effect of diesel fuel on biosynthesis of proteins, carbohydrates and lipids in Chlorella vulgaris Beijer. Biotekhnolohiia. 2011, 4 (6), 74–81. (In Ukrainian).

      23. Lutsiv A. I., Grubinko V. V. Localiztion of the Lipids' Synthesis in Chorella vulgaris under the Impact of Lead and Zinc Ions and Diesel Fuel. Hydrobiol. J. 2012, 48 (6), 95106.


 

Additional menu

Site search

Site navigation

Home Archive 2016 № 4 OPTIMIZATION OF Chlorella vulgaris BEIJ. CULTIVATION IN A BIOREACTOR OF CONTINUOUS ACTION O. I. Bodnar, N. V. Burega, A. O. Palchyk, H. B. Viniarska, V. V. Grubinko

Invitation to cooperation

Dear colleagues, we invite you to publish your articles in our journal.
© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2008.
All rights are reserved. Complete or partial reprint of the journal is possible only with the written permission of the publisher.
E-mail
for information: biotech@biochem.kiev.ua.