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Home Archive 2016 № 1 PIGMENT CONTENT OF Chlorella vulgaris BEIJ. UNDER INFLUENCE OF THE SODIUM SELENITE AND METALS IONS O. I. Bodnar, H. B. Viniarska, O. V. Vasilenko, V. V. Grubinko
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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

"Biotechnologia Acta" V. 9, No 1, 2016
Р. 71-78, Bibliography 27, English
Universal Decimal Classification: 547.979.7+582.263


O. I. Bodnar, H. B. Viniarska, O. V. Vasilenko, V. V. Grubinko

Hnatiuk Ternopil  National Pedagogical University, Ukraine

The aim of the research  was to determine the conditions obtaining in the aquaculture of Chlorella vulgaris Beij. algosubstantion  enriched with selenium and bioactive metals. For this purpose, the content of seaweed pigments studied by the action of sodium selenite in a concentration based on Se4+: 0.5, 5.0, 10.0 and 20.0 mg / dm3 for 1, 3 and 7 days and while exposed 10.0 mg Se4 +/dm3 and Zn2+, Mn2 +, Co2 +, Cu2 +, Fe3 + in concentrations of 5.0 mg/dm 3, 0.25, 0.002, 0.008 and 0.05 mg/dm3, respectively, within 7 days of culturing. The content of pigments was determined spectrophotometrically, the cellular walls were given off in the percoll gradient and investigated microscopically. The pigments content in Ch. vulgaris increase by 1,5–2,5 times in comparison with control sample under the influence of 10 mg Se(IV)/dm3 with and without metal ions. In the same condition a ratio of chlorophylls a/b increased, that accompanied by the formation in cells of the second cell wall as the sign of successful adaptation process in the Chlorella cells under the influence of these factors. Thus, the cultivation of chlorella, enriched with selenium and bioactive  metals, is possible within 7 days under the influence of 10 mg Se (IV)/dm3 and mentioned concentration of these metal ions.

Key words: Chlorella vulgaris Beij., sodium selenite, metal ions, pigments.

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2008

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