Biotechnologia Acta


  • Increase font size
  • Default font size
  • Decrease font size
Home Archive 2016 № 1 NEW GENOTYPES AND TECHNOLOGICAL INDICATORS OF WINTER TRITICALE T. Z. Moskalets, S. P. Vasylkivskyi, B. V. Morgun, V. І. Moskalets, V. V. Moscalets, V. K. Rybalchenko
Print PDF

ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

"Biotechnologia Acta" V. 9, No 1, 2016
Р. 79-86, Bibliography 20, English
Universal Decimal Classification: 577.2:631.526.3:581+351.777.6


T. Z. Moskalets 1, S. P. Vasylkivskyi 1, B. V. Morgun 2, V. І. Moskalets 3,  V. V. Moscalets 1, V. K. Rybalchenko 4

1 Bila Tserkva National Agrarian University, Ukraine
2 Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine, Kyiv
3 Remeslo Nosivka Breeding and Research Station of Myronivka Institute of Wheat of the National Agrarian Academy of Sciences of Ukraine
4 Taras Shevchenko National University of Kyiv, Ukraine

The aim of the research was to conduct basic screening of new lines and cultivars of winter hexaploid triticale by the technological and molecular genetics indicators. Molecular and  genetic research conducted by polymerase chain reaction allelic variants of gene loci Wx-A1, Wx-B1, and quality parameters of grain, flour and bread – on technological markers. The new cultivars and lines of winter hexaploid triticale of Nosivka Breeding and Research Station of Remeslo Myronivka Institute of Wheat by technological indicators of grain, flour and bread quality were studied. According to representative criteria’s the most promising genotypes, which are the main products in terms Forest-Steppe ecotypes’ and  a high-quality raw materials for bakeries and bioethanol were identified. Molecular  and genetic identifications of allelic variants of genes loci Wx-A1, Wx-B1 triticale, which in the early stages of ontogenesis to predict targeted uses genotypes were conducted. The first among a series of triticale cultivars and lines Forest-Steppe ecotypes and biotypes with nonfunctional b gene allele WxA1, which defines a high content of amylopectin of starch, an important release for more ethanol was  identified. It was found that technological characteristics of grain, flour and bread of new cultivars and lines of winter triticale meet the modern requirements production dietetic food and bioenergy products is important and relevant in the context of food security of Ukraine.

Key words: winter triticale, molecular and genetic markers.

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2008

  • References
    • 1. Moskalets T. Z., Moskalets V. V. Autekolohichni ta demekolohichni proiavy modyfikatsiinoi zdatnosti henotypiv tryby Triticeae lisostepovoho ta poliskoho ekotypiv [Population ecology and autecology manifestations of modificational variability of genotypes tribe Triticeae in forest-steppe and polissya ecotypes]. Ecology and noospherology. 2015, 26 (1–2), 41–52. doi: 10.15421/031505. (In Ukrainian).

      2. Rybalka A. Tritikale and energetic. Grain. 2012, 9 (78), 34–37. (In Ukrainian).

      3. Drobot V. I., Fedorova T. O. Research baking properties wholemeal flour of triticale flour and bread manufacturing technology of from this flour. Proceedings of the All-Ukrainian scientific-practic Conference. Poltava, 2003. (In Ukrainian).

      4. Moskalets T. Z., Hrynyk I. V., Tarasiuk S. I., Moskalets V. V., Buniak N. M., Moskalets V. I., Rybalchenko V. K. Modification changeability of new caltivare of wheat-rye amphidiploid for ecological, economic valuable and molecular genetic indicators. J. Problems of Environmental Biotechnology Available at index.php/ecobiotech. (In Ukrainian).

      5. Riabchun V. K., Shatohin V. I., Panchenko I. A. The baking quality of grain of new lines of spring hexaploid triticale. Abstracts of the «Scientific bases stabilization of crop production», Kharkiv, Ukraine. 1999. (In Ukrainian).

      6. Shhipak G. V., Ignatenko A. I. Highly productive cultivar of winter triticale Garne with good baking properties. The Рlant Production Institute nd. a. V.Ya. Yuryev of NAAS. 2010, V. 7, P. 141–147. (In Ukrainian).

      7. Korjachkina S. Ja., Kuznecova E. A., Cherepnina L. V. The Technology of Bread From Whole Grain Triticale. Orel: FGBOU VPOGosuniversitet – UNPK. 2012, 177 p. (In Russian).

      8. Zahra M., Arash F. Molecular diversity and detection of Waxy genes in the Iranian wheat populations by multiplex PCR. Biotechnol. Biotechnol. Equip. 2015, 29 (5), 869–875. doi:10.1080/13102818. 2015.1045940.

      9. Ma J., Stiller J., Zheng Z., Liu Y., Wei Y., Zheng Y., Liu C. A high-throughput pipeline for detecting locus-specific polymorphism in hexaploid wheat (Triticum aestivum L.). Plant Meth. 2015, 11 (39), 14. doi: 10.1186/s13007-015-0082-6.

      10. Vanzetti L. S., Pflüger L. A., Rodrίguez -Quijano M. Genetic variability for waxy genes in Argentinean bread wheat germplasm. J. Biotechnol. Available at http://www. v12n1-2/682. (accessed, August 7, 2008). doi: 10.2225/vol12-issue1-fulltext-2.

      11. Saito M., Konda M., Vrinten P., Nakamura K., Nakamura T., Saito M. Molecular comparison of waxy null alleles in common wheat and identification of a unique null allele. Theor. Appl. Genet. 2004, 108 (7), 1205–1216. PMID: 14689187.

      12. Dospehov B. A. Methodology of field experiences. Moskva: Agropromizdat. 1985, P. 78–143. (In Russian).

      13. Guzmán C., Caballero L., Alvarez J., Yamamori M. Amylose content and starch properties in emmer and durum wheat lines with different waxy proteins composition. Sci. Food Agric. 2011, 91 (9), 32–38. doi: 10.1002/jsfa.4358.

      14. Yamanaka S., Nakamura I., Watanabe K., Sato Y. Identification of SNPs in the waxy gene among glutinous rice cultivars and their evolutionary significance during the domestication process of rice. Theor. Appl. Genet. 2004, 108 (7), 179–184. PMID: 14740088.

      15. Saito M., Vrinten P., Ishikawa G., Graybosch R., Nakamura T. A novel codominant marker for selection of the null Wx-B1 allele in wheat breeding programs. Mol. Breed. 2009, V. 23, P. 209–217. doi:10.1007/s11032-008-9226-y.

      16. Morhun B. V., Stepanenko O. V., Stepanenko A. I., Rybalka O. I. Molecular genetics identification of polymorphisms of Wx genes in hybrid common wheat by using multiplex polymerase chain reaction. Plant Physiology and Genetics. 2015, 47 (1), 25–35. (In Ukrainian).

      17. Ortega R., Alvarez J. B., Guzman C. Characterization of the Wx gene in diploid Aegilops species and its potential use in wheat breeding. Genet. Resources Crop Evol. 2014, 7 (43). doi: 10.1007/s10722-013-0040-y.

      18. Klimushina M. V. Gladkih N. I., Divashuk M. G. The distribution of alleles of genes Wx collection of common wheat Krasnodar Agricultural Research Institute nd. a. P. P. Lukianenko. Vavilov Journal of Genetics and Breeding. 2012, 16 (1), 187–192. (In Russian).

      19. Van K., Onoda S., Kim M. Y., Kim K. D., Lee S.-H. Allelic variation of the Waxy gene in foxtail millet [Setaria italica (L.) P. Beauv.] by single nucleotide polymorphisms. Mol. Genet. Genomics. 2008, V. 279, P. 255–266. doi:10.1007/ s00438-007-0310-5.

      20. Delwiche S., Graybosch R., St Amand P., Bai G. Starch waxiness in hexaploid wheat (Triticum aestivum L.) by NIR reflectance spectroscopy. Agric. Food Chem. 2011, 59 (8), 141. doi: 10.1021/jf104528x.


Additional menu

Site search

Site navigation

Home Archive 2016 № 1 NEW GENOTYPES AND TECHNOLOGICAL INDICATORS OF WINTER TRITICALE T. Z. Moskalets, S. P. Vasylkivskyi, B. V. Morgun, V. І. Moskalets, V. V. Moscalets, V. K. Rybalchenko

Invitation to cooperation

Dear colleagues, we invite you to publish your articles in our journal.
© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2008.
All rights are reserved. Complete or partial reprint of the journal is possible only with the written permission of the publisher.
for information: