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Home Archive 2015 № 4 RESISTANCE OF KARST CAVES MICROORGANISMS TO p-NITROCHLOROBENZENE Suslova O. S., Rokitko P. V., Bondar K. M., Golubenko O. O., Tashyrev A. B.

ISSN 2410-776X (Online),
ISSN 2410-7751 (Print)

Biotechnologia Acta
V. 8, N 4, 2015

"Biotechnologia Acta" V. 8, No 4, 2015
DOI: 10.15407/biotech8.04.135
Р. 135-140, Bibliography 11, English
Universal Decimal Classification: 579.66

RESISTANCE OF KARST CAVES MICROORGANISMS TO p-NITROCHLOROBENZENE

Suslova O. S.1, Rokitko P. V.1, Bondar K. M.2, Golubenko O. O.1, Tashyrev A. B.1

1 Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, Kyiv
2 Taras Shevchenko National University, Kyiv, Ukraine

The aim of this study was to determine homeostasis quantitative parameters (maximum permissible concentrations of xenobiotic and types of cell response) of microbial communities from cave clays influenced by p-nitrochlorobenzene. It was determined the ability of cave bacteria to transform xenobiotic. General bacteriological methods were used as well as gas chromatography-mass-spectric method. Chemoorganotrophic karst caves microbial communities isolated from Mushkarova Yama (Podolia, Ukraine) and Kuybushevskaya (Western Caucasus, Abkhazia) were highly resistant to p-nitrochlorobenzene. For Mushkarova Yama representative strain Rhodococcus erythropolis P3 the influence of p-nitrochlorobenzene (in concentration range 50–300 mg/l) on physiological parameters of bacterial cells was shown. The efficiency of p-nitrochlorobenzene degradation was proportional to the decreasing of redox potential. Thus, cave microorganisms could potentially be used to create new environmentally friendly biotechnologies, for example enterprises for wastewater treatment from nitrochloraromatic compounds.

Key words: karst caves, microbial communities, p-nitrochlorobenzene.

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2008

  • References
    • 1. Gvozdyak P. I., Mogilevich N. F., Tashirev A. B. Transformation of p-nitrohlorobenzene by E. coli. Microbiologiya. 1983, 52 (1), 22–25. (In Russian).

      2. Tashirev A. B., Matveeva N. A., Romanovskaya V. A., Tashireva A. A. Rokitko P. V.Polyresistance superstability and heavy metals Antarctic microorganisms. Dopovіdі Natsіonalnoi Akademіi nauk Ukrainy. 2007, V. 11, P. 170–175. (In Russian).

      3. Tashirev A. B., Rokitko P. V., Levishko A. S., Romanovskaya V. A., Tashireva A. A. Resistance to toxic metals of chemoorganotrophic bacteria isolated from the Antarctic cliffs. Mіkrobіol. zh. 2012, 74 (2), 3–7. (In Russian).

      4. Tashirev A. B., Romanovskaya V. A., Rokitko P. V., Tashireva A. A. Multiple resistance to toxic metals of Antarctic cliffs microorganisms (o. Galindez). Ukr. antarkt. zh. 2011–2012, V. 11–12, P. 212–221. (In Russian).

      5. Tashirev A. B., Tihnenko S. A. Transformation of p-ntrohlorbenzene by obligate anaerobic microorganisms. Abstracts of 2 Union Conference “Microbiology of water treatment”, Kyiv. 1982, 194 p. (In Russian).

      6. Chirayu Desai, Hilor Pathak, Datta Madamwar. Advances in molecular and “-omics” technologies to gauge microbial communities and bioremediation at xenobiotic/anthropogen contaminated sites. Biores. Technol. 2010, 101 (6), 1558–1569. doi: 10.1016/j.biortech.2009.10.080.

      7. Li Haixiang, Yang Xin, Zhong Fohua, Li Junying, Xia Siqing. Experimental study of para-nitrochlorobenzene (p-NCB) removal from groundwater by autohydrogenotrophic microorganism. Chin. J. Environ. Engin. 2011, V. 2, P. 43–48.

      8. Suslova Оlga, Govorukha Vera, Brovarskaya Оksana, Matveeva Nadezhda, Tashyreva Hanna, Tashyrev Oleksandr. Method for determining organic compound concentration in biological systems by permanganate redox titration. Int. J. Bioautomat. 2014, 18 (1), 45–52.

      9. Longjiang Zhanga, Xin Wangb, Yiying Jiaob, Xu Chenb, Lingyan Zhouc, Kun Guoc, Feng Ge. Biodegradation of 4-chloronitrobenzene by biochemical cooperation between Sphingomonas sp. Strain CNB3 and Burkholderia sp. strain CAN6 isolated from activated sludge. Chemosphere. 2013, 91 (9), 1243–1249. doi: 10.1016/j.chemosphere.2013.01.115.

      10. Wua Haizhen, Weib Chaohai, Wanga Yaqin, Heb Qincong, Liang Shizhong. Degradation of o-chloronitrobenzene as the sole carbon and nitrogen sources by Pseudomonas putida OCNB-1. J. Environ. Sci. 2009, 21 (1), 89–95. doi: 10.1016/S1001-0742(09)60016-4.

      11. Arora Pankaj Kumar, Sasikala Ch., Ramana Ch. Venkata. Degradation of chlorinated nitroaromatic compounds. Appl. Microbiol. Biotechnol. 2012, 93 (6), 2265–2277.
      doi: 10.1007/s00253-012-3927-1.


 

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Home Archive 2015 № 4 RESISTANCE OF KARST CAVES MICROORGANISMS TO p-NITROCHLOROBENZENE Suslova O. S., Rokitko P. V., Bondar K. M., Golubenko O. O., Tashyrev A. B.

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