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Home Archive 2015 № 2 Bacillus amyloliquefaciens SUBSP. plantarum PROBIOTIC STRAINS AS PROTEASE PRODUCERS E. V. Маtseliukh, L. А. Safronova, L. D. Varbanets
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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

Biotechnologia Acta
V. 8, No 2, 2015

"Biotechnologia Acta" V. 8, No 2, 2015;  
doi: 10.15407/biotech8.02.084
Р. 84-90, Bibliography 20, English
Universal Decimal classification: 577.152.34:577.151.5

Bacillus amyloliquefaciens SUBSP. plantarum PROBIOTIC STRAINS AS PROTEASE PRODUCERS

E. V. Маtseliukh, L. А. Safronova, L. D. Varbanets

Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, Kyiv

Proteases from probiotic strains of the genus Bacillus, just like the antibiotics, bacteriocins and other hydrolytic enzymes, are one of the main factors that determine their biological activity. The aim of this work was to study the synthesis and biochemical properties of proteases from two strains Bacillus amyloliquefaciens subsp. plantarum UCM B-5139 and UCM B-5140 that included in the probiotic Endosporin. The cultivation of strains was carried out in flasks under rotating for two days. The influence of physico-chemical parameters of the reaction medium on proteolytic activity was studied on partially purified protease preparations. Lytic activity was determined by turbidimetric method. On the second day of cultivation B. amyloliquefaciens subsp. plantarum UCM В-5139 and UCM В-5140 synthesized the metal-dependent peptidase and serine protease, respectively. The optimum conditions of their action were the following: temperature 37–40 °C and pH 6.5–7.0. Isolated proteases are able to lyse the living cells of Staphylococcus aureus and Candida albicans. Thus we demonstrated that B. amyloliquefaciens subsp. plantarum UCM B-5140 and UCM B-5139, included in the probiotic veterinary preparation Endosporin, produced proteolytic enzymes that hydrolyze the native insoluble proteins (elastin, fibrin and collagen). These enzymes belong to the group of neutral metal-dependent and serine proteases. They are active under physiological conditions against gram-positive bacteria and yeasts. The application of these proteases in biotechnology is considered.

Key words: Bacillus amyloliquefaciens subsp. plantarum, probiotics, proteases, lytic activity.

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2008

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Home Archive 2015 № 2 Bacillus amyloliquefaciens SUBSP. plantarum PROBIOTIC STRAINS AS PROTEASE PRODUCERS E. V. Маtseliukh, L. А. Safronova, L. D. Varbanets

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