ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)
"Biotechnologia Acta" V. 9, No 4, 2016
https://doi.org/10.15407/biotech9.04.050
Р. 50-57, Bibliography 20, English
Universal Decimal Classification: 616-006.004.576.31
THE CULTIVATION OF RAT COLON TUMOR WITH PLACENTAL MULTIPOTENT STEM CELLS
H. M. Svitina 1, 2, O. А. Rybachuk 3, 4, 5, L.V. Garmanchuk 1, V. A. Shablii 2, G. S. Lobyntseva 2
1 Educational and Scientific Centre “Institute of Biology”, Taras Shevchenko National University of Kyiv, Ukraine
2 Institute of Cell Therapy, Kyiv, Ukraine
3 Bogomoletz Institute of Physiology of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
4 State Institute of Genetic and Regenerative Medicine of the National Academy of Medical Sciences of Ukraine, Kyiv
5 Biotechnology laboratory ilaya.regeneration, Medical company ilaya, Kyiv, Ukraine
The aim of the research was to assess the impact of rat placental multipotent stem cells on the levels of proliferation and apoptosis in organotypic coculture with slices of colon tumors. Placental multipotent stem cells were isolated by explant method, colon cancer was modeled by administering 20 mg/kg dimethylhydrazine weekly for 20 weeks, tumors of the colon were cut into 500 µm slices. There were no changes in proliferative activity of tumor tissue, or in the level of apoptosis. Absence of influence of placental multipotent stem cells on colon tumor growth is a unique feature of these cells, and extends the application of placental multipotent stem cells in therapy.
Key words: placental multipotent stem cells, colon cancer, organotypic cocultivation.
© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2016
References
1 Ouakrim D. A., Pizot C., Boniol M., Malvezzi M., Boniol M., Negri E., Bota M., Jenkins M. A., Bleiberg H., Autier Ph. Trends in colorectal cancer mortality in Europe: retrospective analysis of the WHO mortality database. BMJ. 2015, 351:h4970. https://doi.org/10.1136/bmj.h4970
2 Zavoral M., Suchanek S., Zavada F., Dusek L., Muzik J., Seifert B., Fric P. Colorectal cancer screening in Europe. World J. Gastroenterol. 2009, 15 (47), 5907–5915. https://doi.org/10.3748/wjg.15.5907
3 American Cancer Society. Colorectal Cancer Facts & Figures 2014–2016. Atlanta: American Cancer Society. 2014.
4 Armaghany T., Wilson J. D., Chu Q., Mills G. Genetic alterations incolorectal cancer. Gastrointest. Cancer Res. 2012, V. 5, P 19–27.
5 Mouradov D., Sloggett C., Jorissen R. N., Love C. G., Li S., Burgess A. W., Arango D., Strausberg R. L., Buchanan D., Wormald S., O'Connor L., Wilding J. L., Bicknell D., Tomlinson I. P., Bodmer W. F., Mariadason J. M., Sieber O. M. Colorectal cancer cell lines are representative models of the main molecular subtypes of primary cancer. Cancer Res. 2014, 74 (12), 3238–3247. https://doi.org/10.1158/0008-5472.CAN-14-0013
6 Vu B., Phan N., Pham P. Mesenchymal Stem Cells: vector for targeted cancer therapy. Progr. Stem Cell. 2016, 3 (1), 73–86. https://doi.org/10.15419/psc.v3i01.122
7 Kassem M., Kristiansen M., Abdallah B. M. Mesenchymal stem cells: cell biology and potential use in therapy. Bas. Clin. Pharmacol. Toxicol. 2004, 95 (5), 209–214. https://doi.org/10.1111/j.1742-7843.2004.pto950502.x
8 Miao Z., Jin J., Chen L., Zhu J., Huang W., Zhao J., Qian H., Zhang X. Isolation of mesenchymal stem cells from human placenta: comparison with human bone marrow mesenchymal stem cells. Cell Biol. Int. 2006, 30 (9), 681–687. https://doi.org/10.1016/j.cellbi.2006.03.009
9 Zhong Z., Kusznieruk K. P., Popov I. A., Riordan N. H., Izadi H., Yijian L., Sher S., Szczurko O. M., Agadjanyan M. G., Tullis R. H., Harandi A., Reznik B. N., Mamikonyan G. V., Ichim T.E. Induction of antitumor immunity through xenoplacental immunization. J. Transl. Med. 2006, 25 (4), 22. https://doi.org/10.1186/1479-5876-4-22
10 Zhao B., Wang Y., Wu B., Liu S., Wu E., Fan H. X., Gui M. M., Chen L., Li Ch., Ju Y., Zhang W., Meng S. Placenta-derived gp96 as a multivalent prophylactic cancer vaccine. Sci. Rep. 2013, 3, Article number: 1947. https://doi.org/10.1038/srep01947
11 Lee S-L., Hsu S-L. Placental preparation having antitumor activity. U. S. Patent 2004/0076618 A1. Apr. 22, 2004.
12 Svitina H., Kalmukova O., Shelest D., Skachkova O., Garmanchuk L., Shablii V. Cellular immune response in rats with 1,2-dimethylhydrazine-induced colon cancer after transplantation of placenta-derived stem multipotent cells. Cell Org. Transplantol. 2016, 4 (1), 48–60. https://doi.org/10.22494/cot.v4i1.7
13 Per?e M., Cerar A. Morphological and molecular alterations in 1,2 dimethylhydrazine and azoxymethane induced colon carcinogenesis in rats. J. Biomed. Biotechnol. 2011, V. 2011, P 473964. https://doi.org/10.1155/2011/473964
14 Corr?a M. P., Ferreira A. P., Gollner A. M., Rodrigues M. F., Guerra M. C. Markers expression of cell proliferation and apoptosis in basal cell carcinoma. Bras. Dermatol. 2009, 84 (6), 606–614. https://doi.org/10.1590/S0365-05962009000600006
15 Kubben F. J., Peeters-Haesevoets A., Engels L. G., Baeten C. G., Schutte B., Arends J. W., Stockbr?gger R. W., Blijham G. H. Proliferating cell nuclear antigen (PCNA): a new marker to study human colonic cell proliferation. Gut. 1994, 35 (4), 530–535. https://doi.org/10.1136/gut.35.4.530
16 Vermeulen K., Berneman Z. N., van Bockstaele D. R. Cell cycle and apoptosis. Cell Prolif. 2003, 36 (3),165–175. https://doi.org/10.1046/j.1365-2184.2003.00267.x
17 Boeck A.D. Bone marrow derived mesenchymal stem cells in colorectal cancer: neuregulin at the invasion front. Belg. J. Med. Oncol. 2014, 8 (2), 52–54.
18 Hogan N. M., Joyce M. R., Murphy J. M., Barry F. P., O'Brien T., Kerin M. J., Dwyer R. M. Impact of mesenchymal stem cell secreted PAI-1 on colon cancer cell migration and proliferation. Biochem. Biophys. Res. Commun. 2013, 435 (4), 574–579. https://doi.org/10.1016/j.bbrc.2013.05.013
19 Dzobo K., Vogelsang M., Thomford N. E., Dandara C., Kallmeyer K., Pepper M. S., Parker M. I. Wharton's Jelly-Derived Mesenchymal Stromal Cells and Fibroblast-Derived Extracellular Matrix Synergistically Activate Apoptosis in a p21-Dependent Mechanism in WHCO1 and MDA MB 231 Cancer Cells In Vitro. Stem Cells Int. 2016, 2016:4842134. https://doi.org/10.1155/2016/4842134
20 Khakoo A. Y., Pati S., Anderson S. A., Reid W., Elshal M. F., Rovira I. I., Nguyen A. T., Malide D., Combs C. A., Hall G., Zhang J., Raffeld M., Rogers T. B., Stetler-Stevenson W., Frank J. A., Reitz M., Finkel T. Human mesenchymal stem cells exert potent antitumorigenic effects in a model of Kaposi's sarcoma. J. Exp. Med. 2006, 203 (5), 1235–1247. https://doi.org/10.1084/jem.20051921