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Home Archive 2014 № 6 THE RAPID DIAGNOSTICS OF SEX OF SALMONIDS USING DNA-MARKERS Yu. P. Rud, L. P. Buchatsky
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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

"Biotechnologia Acta" v. 7, no 6, 2014
doi: 10.15407/biotech7.06.017
Р. 17-22, Bibliography 14, English
Universal Decimal classification: [597-146.3:597-146.5]:597.553.2

THE RAPID DIAGNOSTICS OF SEX OF SALMONIDS USING DNA-MARKERS

Yu. P. Rud1, L. P. Buchatsky2

1Institute of Fisheries of the National Academy of Agrarian Sciences of Ukraine, Kyiv
2Taras Shevchenko National University of Kyiv, Ukraine

Based on nucleotide sequences of sex-specific DNA-markers of salmonid fishes the oligonucleotide primers for polymerase chain reaction were selected with purpose on rapid diagnostic of sex in rainbow trout Onchorhynchus mykiss, brown trout Salmo trutta, huchen Hucho hucho and grayling Thymallus thymallus. The specify of amplification was determined by nucleotide sequence analysis of PCR-products. All amplified fragments were referred to sex-specific locuses of Y chromosomes in males of investigated fish species. The PCR-products were in size of 880, 607, 521 and 558 for rainbow trout, brown trout, grayling and huchen respectively. Thus the sex determination in above mentioned fish species and identification of genotypic males under process of hormonal sex reversion can be provided using conventional PCR. Present method relates to rapid diagnostics because the data analysis and return of results back to fish farm take one single day.

Key words: DNA-markers, PCR diagnostics, sex reversion, salmon raising.

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2008

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Home Archive 2014 № 6 THE RAPID DIAGNOSTICS OF SEX OF SALMONIDS USING DNA-MARKERS Yu. P. Rud, L. P. Buchatsky

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