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Home Archive 2014 №5 CULTIVATION OF NEURAL CREST-DERIVED MULTIPOTENT STEM CELLS IN COLLAGEN AND FIBRIN HYDROGELS: EFFECTS ON CELL VIABILITY AND PROLIFERATION Vasyliev R. G., Zubov D. A., Rodnichenko A. E., Labunets I. F., Novikova S. N.
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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

Ж-л "Biotechnologia Acta"
V. 7, No 5, 2014

"Biotechnologia Acta" v. 7, no 5, 2014
doi: 10.15407/biotech7.05.050
Р. 50-54, Bibliography 9, English.
Universal Decimal classification: 576.3+612.014.2/3

CULTIVATION OF NEURAL CREST-DERIVED MULTIPOTENT STEM CELLS IN COLLAGEN AND FIBRIN HYDROGELS: EFFECTS ON CELL VIABILITY AND PROLIFERATION

Vasyliev R. G.1, 2, Zubov D. A.1, 2, Rodnichenko A. E.1, 2, Labunets I. F.1, Novikova S. N.1

1State Institute of Genetic and Regenerative Medicine of the National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine
2Biotechnology laboratory ilaya. regeneration, Medical company ilaya, Kyiv, Ukraine

The possibility of culture of neural crest-derived multipotent stem cells in 3D collagen and fibrin hydrogels has been shown. The effects on cell viability and growth have been explored. It is shown that both hidrogels contribute to cell adhesion and spreading. During culture it maintains high cell viability in both types of hydrogels as well as network formation of the interconnected cells. When cultured for two weeks in a collagen hydrogel cell number increased by 1.4 fold, while the fibrin hydrogel shows almost a threefold increase in cell number. The results obtained can be used for development of neural crest-derived multipotent stem cell-based tissue-engineered constructs.

Key words: neural crest-derived multipotent stem cells, 3D culture, hydrogel, fibrin, collagen.

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2008

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Home Archive 2014 №5 CULTIVATION OF NEURAL CREST-DERIVED MULTIPOTENT STEM CELLS IN COLLAGEN AND FIBRIN HYDROGELS: EFFECTS ON CELL VIABILITY AND PROLIFERATION Vasyliev R. G., Zubov D. A., Rodnichenko A. E., Labunets I. F., Novikova S. N.

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