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"Biotechnologia Acta" V. 9, No 5, 2016
https://doi.org/10.15407/biotech9.05.018
Р. 18-23, Bibliography  11, English
Universal Decimal Classification: 615.017: 576.385.5

 5 2016

NANOCOMPOSITE COMPLEX  EMAP II INFLUENCE  ON   TUMOR NECROSIS FACTOR AND INTERFERON

L. A. Kolomiets-Babenko 1, 2, O. S. Bohorad-Kobelska 3, N. L. Kovalchuk 2, M. Ja. Spivak 3, A. I. Kornelyuk 1

1 Institute of Molecular Biology and Genetics, of the National Academy of Sciences of Ukraine
2 Taras Shevchenko National University of Kyiv, Ukraine
3 Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine

The goal of the research was to determine the ability of new nanocomposite preparation EMAP II (endothelial  monocyte  activating  poplypeptide  II)  to  affect  the  expression of  the  tumor-necrosis factor and interferon in vitro. In the experiments, the transformed cell line L929 cells was used. The induced  interferon  levels  were  determined  in  samples  of  culture  medium  by  the  microtitration method in the L929 cell culture against test virus  vesicular stomatitus VSV. Toxicity of the substance was assessed by its maximum tolerated dose. The amount of endotoxins in nanocomposite preparation EMAP II was measured using gel-clot test.  The range of concentrations of EMAP II causing the production of tumor necrosis factor was determined. The concentration of lipopolysaccharides in the tested nanocomposite preparation was less then 0.5 IEU/kg. New nanocomposite preparation EMAP II has the ability to induce TNF-α production at rather low concentration 1.56–25.00 ?g/ml (82.49–1370.00 mol х 10–12). The interferon production under the influence of nanocomposite preparation EMAP II was not found. The results support the application of the target nanocomposite reparation EMAP II for cancer treatment.

Key words: nanocompomposite EMAP II, tumor-necrosis factor TNF-α, interferon.

© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2016