Biotechnologia Acta


  • Increase font size
  • Default font size
  • Decrease font size
Print PDF

ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

Biotechnologia Acta
v. 6, No. 6, 2013

"Biotechnologia Acta" v. 6, no 6, 2013
doi: 10.15407/biotech6.06.045
Р. 45-57, Bibliography 90, Russian.
Universal Decimal classification: 621.59: 597 114.78


L. P. Buchatsky

Institute for Fisheries of Ukrainian Academy of Agrarian Sciences, Kyiv, Ukraine

The latest progress in biotechnology on fish aquaculture and different modern methods of investigations for increasing of fish productivity in aquaculture are analyzed. Except for the applied aspect, the use of modern biotechnological methods of investigations opens new possibilities for fundamental researches of sex-determining mechanisms, polyploidy, distant hybridization, and developmental biology of bony fishes. Review contains examples of utilizing modern biotechnology methods to obtain transgenic fishes with accelerated growth and for designing surrogate fishes. Methods for receiving unisexual shoals of salmon and sturgeon female fishes with the view of obtaining a large quantity of caviar, as well as receiving sterile (triploid) fishes are analyzed. Great attention is given to androgenesis, particularly to disperm one, in connection with the problem of conserving rare and vanishing fish species using only sperm genetic material. Examples how distant hybrids may be obtained with the use of disperm androgenesis and alkylated DNA are given. Methods of obtaining fish primordium germ cells, recent developments in cultivation of fish stem cells and their use in biotechnology, as well as ones of transplantation of oogonium and spermatogonium to obtain surrogate fishes. The examples of successful experiments on spermatogonial xenotransplantation and characteristic of antifreezing fish proteins and also the prospect of their practical usage are given.

Key words: fishes, grows acceleration, reproduction, sterilization, androgenesis, stem cells, transplantation, antifreeze proteins.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008

  • References
    • 1. Vasil I. K. Biotechnology and food security for the 21st century: a real-world perspective. Nat. Biotechnol. 1998, V. 16, P. 399–400.

      2. Hew C. L., Fletcher G. L. The role of aquatic biotechnology in aquaculture. Aquaculture. 2001, V. 197, P. 191-204.

      3. Aparicio S., Chapman J., Stupka E. Whole-genome shotgun assembly and analysis of the genome of Fugu rubripes. Science. 2002, 297 (5585), 1301-1310.

      4. DaSilva E. J. The colours of  Biotechnology: Science, Development and Humankinde. Electr. J. Biotechnol. Edit. art. 2004, 7 (3), 1.

      5. Palmiter R. D., Brinste R. L., Hammer R. E. Dramatic growth of mice that develop from eggs microinjected with metallothionein-growth hormone fusion genes. Nature. 1982, V. 300, P. 611–615.

      6. Du S. J., Gong Z., Fletcher G. L. Growth enhancement in transgenic Atlantic salmon by the use of an all fish chimeric growth hormone gene construct. Biol. Technol. 1992, 10 (2), 176–181.

      7. Rasmussen R. S., Morrissey M. T. Biotechnology in aquaculture: transgenic and polyploidy. Comprehensive reviews in food science and food safety. 2007, V. 6, P. 2-16.

      8. Wan H., He J., In B. Generation of two-color transgenic zebrafish using the green and red flguorescent protein receptor genes, gfp and rfp. Mar. Biotechnol. 2002, 4 (2), 146-154.

      9. Takeuchi Y., Yoshizaki G., Kobayashi T. Mass isolation of primordial germ cells from transgenic rainbow trout carrying the green fluorescent protein gene driven by the vasa gene promoter. Biol. Reprod. 2002, V. 67, P. 1087-1092.

      10. Yoshizaki G., Tago Y., Takeuchi Y. Green fluorescent protein labeling of primordial germ cells using a nontransgenic method and its application for germ cell transplantation in salmonidae. Biol. Reprod. 2005, V. 73, P. 88-93.

      11. Yamaha E., Saito T., Goto-Cazeto R. Developmental biotechnology for aquaculture, with special reference to surrogate production in teleost fishes. J. Sea Res. 2007, V. 58, P. 8-22.

      12. Shikina S., Ihara S., Yoshizaki G. Culture conditions for maintaining the survival and mitotic activity of rainbow trout transplantable type A spermatogonia. Mol. Reprod. Dev. 2008, V. 75, P. 529-537.

      13. Alvarez M. C., Bejar J., Chen S. Fish ES cell and applications to biotechnology. Marine Biotechnol. 2007, V. 9, P. 117-127.

      14. Buchatskyi L. P. Fish Transgenesis. Rybn. gospHomelskyi. 2000, V. 56-57, P. 51-61.

      15. Devlin R. H., Nagahama Y. Sex determination in fish: an overview of genetic, physiological, and environmental influences. Aquaculture. 2002, V. 208, P. 191-364.

      16. Devlin R. H., Park L., Sakhrani D. M. Variation of Y-chromosome markers in chinoock salmon (Oncorhynchus tshawytscha) populations. Can. J. Fish. Aquat. Sci. 2005, V. 62, P. 1386-1399.

      17. Brunelli J. P., Wertzler K. J., Sundin K. Y-specific sequences and polymorphisms in rainbow trout and chinoock salmon. Genome. 2008, V. 51, P. 739-748.

      18. Lanes C. F., Sampaio L. A., Marins L. F. Evaluation of DNAse activity in seminal plasma and uptake of exogenous DNA by spermatozoa of the brazilian flounder Paralichthys orbignyanus. Theriogenology. 2009, V. 71, P. 525-533.

      19. Maclean N., Laight, R. J. Transgenic fish: an evaluation of benefits and risks. Fish. 2000, N 1, P. 146–172.

      20. Homelskyi B. I., Hrunina A. S. Artificial polyploidy fish and possibility of its use in fish farming. TSNIIITEIRKh Overview. 1988, V. 1, P. 1-25. (In Russian).

      21. Horbunov L. V., Buchatskyi L. P. Cryopreservation of gametes and embryos of animals. VPTs Kyiv. un-tet. 2005, 325 p. (In Russian).

      22. Grunina A. S., Recoubratsky A. V., Neyfakh A. A. Induced diploid androgenesis in sturgeons. Sturgeon Quart. 1995, 3 (3), 6–7.

      23. Araki K., Shinma H., Nagoya H. Androgenetic diploids of rainbow trout (Oncorhynchus mykiss) produced by fused sperm. Can. J. Fish. Aquat. Sci. 1995, V. 52, P. 892–896.

      24. Hrunina A. S., Rekurbatskiy A.V. Androgenesis fish, or only the seed from the male. Priroda. 2006, N 11, P. 25–31. (In Russian).

      25. Hrunina A. S., Rekurbatskiy A.V., Tsvetkova L. I. Dispermic androgenesis sturgeons using cryopreserved sperm: obtaining androgenetic offspring of the Siberian sturgeon and androgenetic hybrids between Siberian and Russian sturgeon. Ontogenez. 2011, 42 (2), 133–145. (In Russian).

      26. Kirankumar S., Pandian T. J. Use of heterologous sperm for the dispermic induction of androgenesis in barbs. J. Fish Biol. 2004, V. 64, P. 1485–1497.

      27. Clifton J. D, Pandin T. J. Dispermic induction of intercpecific androgenesis in the fish, Buenos Aires tetra using surrogate eggs widow tetra. Curr. Sci. 2008, 95 (1), 64.

      28. Buchatskii L.P., Potopalskyi A.I., Zaika L.A. The use of alkylated DNA hybrids remote to display fish. Tvarinnitstvo Ukr. 2010, N 4, P. 21–23. (In Ukrainian).

      29. Buchatskii L.P., Potopalskii A. I, Zaika L.A. The patent for utility model N 44303 “Method for hybrids fish obtaining”. Registered 25.09.2009.  (In Ukrainian).

      30. Melamed P., Gong Z., Fletcher G. The potential impact of modern biotechnology on fish aquaculture. Aquaculture. 2002, V. 204, P. 255–269.

      31. Yoshizaki G., Ichikawa M., Hayashi M. Sexual plasticity of ovarian germ cells in rainbow trout. Development. 2010, V. 137, P. 1227–1230.

      32. Metalnikova K. V. Progeny reversantov steelhead. Ribn. khoz. 1991, N 12, P. 59–61. (In Russian).

      33. Pavlov E. D., Nhuen Vet Tui, Nhuen Ti Tu. Status of sexual glands of young triploid trout Oncorhynchus mykiss in conditions of Southern Vietnam during artificial inversion of sex. Vopr. ikhtiol. 2010, 50 (5), 675–684. (In Russian).

      34. Raz E., Reichman-Fried M. Attraction rules: germ cell migration in zebrafish. Curr. Opin. Genet. Develop. 2006, V. 16, P. 355–359.

      35. Yoshizaki G., Sakatani S., Tominaga H. Cloning and characterization of a vasa-like gene in rainbow trout and its expression in the germ cell lineage. Mol. Reprod. Develop. 2000, V. 55, P. 364–371.<364::AID-MRD2>3.0.CO;2-8

      36. Takashima F., Patino R., Nomura M. Histological studies on the sex differentiation in rainbow trout. Bull. Japan. Soc. Sci. Fish. 1982, V. 46, P. 1317–1322.

      37. Raz E. The function and regulation of vasa-like genes in germ-cell development. Genome Biol. 2000, N 1, P. 1017.

      38. Yoshizaki G., Takeuchi Y., Sakatani S. Germ cell–specific expression of green fluorescent protein in transgenic rainbow trout under control of the rainbow trout vasa–like gene promoter. Int. J. Dev. Biol. 2000, V. 44, P. 323–326.

      39. Yoshizaki G., Fujinuma K., Iwasaki Y. Spermatogonial transplantation in fish: a novel method for the preservation of genetic resources. Comp. Biochem. Physiol. Part D. Genom. Proteom. 2011, 6 (1), 55–61.

      40. Knaut H., Steinbeisser H., Schwarz H. An evolutionary conserved region in the vasa 3′UTR targets RNA translation to the germ cells in the zebrafish. Curr. Biol. 2002, N 12, P. 454–466.

      41. Wolkе U., Weidinger G., Köprunner M. Multiple levels of posttranscriptional control lead to germ line-specific gene expression in the zebrafish. Curr. Biol. 2002, V. 12, P. 289–294.

      42. Okutsu T., Suzuki K., Takeuchi Y. Testicular germ cells can colonize sexually undifferentiated embryonic gonad and produce functional eggs in fish. Proc. Natl. Acad. Sci. USA. 2006, V. 103, P. 2725–2729.

      43. Yano A., Suzuki K., Yoshizaki G. Flowcytometric isolation of testicular germ cells from rainbow trout (Oncorhynchus mykiss) carrying the greenfluorescent protein gene driven by trout vasa regulatory regions. Biol. Reprod. 2008, V. 78, P. 151–158.

      44. Schulz R. W, de França L. R., Lareyre J. J. Spermatogenesis in fish. Gen. Comp. Endocrinol. 2010, V. 165, P. 390–411.

      45. Yoshizaki G., Ichikawa M., Hayashi M. Sexual plasticity of ovarian germ cells in rainbow trout. Development. 2010, V. 137, P.1227–1230.

      46. Loir M. Spermatogonia of rainbow trout: I. Morphological characterization, mitotic activity, and survival in primary cultures of testicular cells. Mol. Reprod. Dev. 1999, V. 53, P. 422–433.<422::AID-MRD8>3.0.CO;2-V

      47. Okutsu T., Yano A., Nagasawa K. Manipulation of fish germ cell: visualization, cryopreservation and transplantation. J. Reprod. Develop. 2006, V. 52, P. 685–693.

      48. Okutsu T., Shikina S., Kanno M. Production of trout offspring from triploid salmon parents. Science. 2007, V. 317, P. 1517.

      49. Okutsu T., Takeuchi Y., Yoshizaki G. Spermatogonial transplantation in fish: production of trout offspring from salmon parents. Tsukamoto K. (Ed). Fisheries for global welfare and environment. 5th World Fisheries Congress. 2008, P. 209–219.

      50. Lacerda S. M., Batlouni S. R., Silva S. B. Germ cell transplantation in fish: the Nile–tilapia model. Anim. Reprod. 2006, N 3, P. 146–159.

      51. Lacerda S. M., Batlouni S. R., Assis L. Germ cell transplantation in tilapias (Oreochromis niloticus). Cybium. 2008, V. 32, P. 115–118.

      52. Lacerda S. M., Batlouni S. R., Costa G. M. A new and fast technique to generate offspring after germ cells transplantation in adult fish: the nile tilapia (Oreochromis niloticus) model. PLoS One. 2010, N 5, e 10740.

      53. Majhi S. K., Hattori R. S., Yokota M. Germ cell transplantation using sexually competent fish: an approach for rapid propagation of endangered and valuable germlines. PLoS One. 2009, N 4, e 6132.

      54. Nagasawa K., Shikina S., Takeuchi Y. Lymphocyte antigen 75 (Ly75/CD205) is a surface marker on mitotic germ cells in rainbow trout. Biol. Reprod. 2010, 83 (4), 597–606.

      55. Hong Y., Liu T., Zhao H. Establishment of a normal medaka fish spermatogonial cell line capable of sperm production in vitro. Proc. Natl. Acad. Sci. USA. 2004, V. 101, P. 8011–8016.

      56. Carrasco L. A., Doroshov S., Penman D. J. Long-term, quantitative analysis of gametogenesis in autotriploid rainbow trout, Oncorhynchus mykiss. J. Reprod. Fertil. 1998, V. 113, P. 197–210.

      57. Lin S., Long W., Chen J. Production of germ-line chimeras in zebrafish by cell transplants from genetically pigmented to albino embryos. Proc. Natl. Acad. Sci. USA. 1992, V. 89, P. 4519–4523.

      58. Nakamura S., Kobayashi K., Nishimura T. Identification of germline stem cells in the ovary of the teleost medaka. Science. 2010, V. 328, P. 1561–1563.

      59. Nobrega R. H., Batlouni S. R., Franca L. R. An overview of functional and stereological evaluation of spermatogenesis and germ cell transplantation in fish. Fish Physiol. Biochem. 2009, V. 35, P. 197–206.

      60. Collares T., Campos V. F., Seixas F. K. Transgene transmission in south american catfish (Rhamdia quelen) larvae by sperm–mediated gene transfer. J. Biosci. 2010, V. 35, P. 39–47.

      61. Collodi P., Kamei Y., Ernst T. Culture of cells from zebrafish (Brachydanio rerio) embryo and adult tissues. Cell. Biol. Toxicol. 1992, N 8, P. 43–61.

      62. Yi M., Hong N., Hong Y. Generation of medaka fish haploid embryonic stem cells. Science. 2009, V. 326, P. 430–433.

      63. Sun L., Bradford C. S., Ghosh C. Es-like cell cultures derived from early zebrafish embryos. Mol. Mar. Biol. Biotechnol. 1995, N 4, P. 193–199.

      64. Fan L., Crodian J., Collodi P. Culture of embryonic stem cell lines from zebrafish. Meth. Cell Biol. 2004, V. 76, P. 151–160.

      65. Ma C., Fan L., Ganassin R. Production of zebrafish germ–line chimeras from embryo cell cultures. Proc. Natl. Acad. Sci. USA. 2001, V. 98, P. 2461–2466.

      66. Alvares M. C., Bejar J., Chen S. Fish ES cells and applications to biotechnology. Marine Biotechnol. 2006, V. 9, P. 117–127.

      67. Li Z., Bhat N., Manali D. Medaka cleavage embryos are capable of generating ES–like cell cultures. Int. J. Biol. Sci. 2011, N 7, P. 418–425.

      68. Yi M., Hong N., Hong Y. Generation of medaka fish haploid embryonic stem cells. Science. 2009, V. 326, P. 430–433.

      69. Hong N., Li Z., Hong Y. Fish stem cell cultures. Int. J. Biol. Sci. 2011, 7 (4), 392–402.

      70. Shikina S., Yoshizaki G. Improved in vitro culture conditions to enhance the survival, mitotic activity, and transplantability of rainbow trout type A spermatogonia. Biol. Reprod. 2010, V. 83, P. 268–276.

      71. Bejar J., Hong Y., Alvarez M. C. An ES–like cell line from the marine fish sparus aurata: characterization and chimaera production. Transgenic Res. 2002, V. 11, P. 279–289.

      72. Chen S. L., Ye H., Sha Q. Derivation of a pluripotent embryonic cell line from red sea bream blastulas. J. Fish. Biol. 2003, V. 63, P. 10.

      73. Chen S. L., Sha Z. X., Ye H. Q. Pluripotency and chimera competence of an embryonic stem cell line from the sea perch (Lateolabrax japonicus). Mar. Biotechnol. 2007, V. 9, P. 82–91.

      74. Parameswaran V., Shukla R., Bhonde R. Development of a pluripotent ES–like cell line from Asian sea bass (Lates calcarifer) – an oviparous stem cell line mimicking viviparous es cells. Mar. Biotechnol. 2007, V. 9, P. 766–775.

      75. Holen E., Hamre K. Towards obtaining long term embryonic stem cell like cultures from a marine flatfish, Scophthalmus maximus. Fish. Physiol. Biochem. 2004, V. 29, P. 245–252.

      76. Holen E., Kausland A., Skjaerven K. Embryonic stem cells isolated from atlantic cod (Gadus morhua) and the developmental expression of a stage–specific transcription factor ac-pou2. Mar. Biotechnol. 2010, 36 (4), 1029–39.

      77. Campbell K. H., McWhir J., Ritchie W. A. Sheep cloned by nuclear transfer from a cultured cell line. Nature. 1996, V. 380, P. 64–66.

      78. Chen H., Yi Y., Chen M. Studies on the developmental potentiality of cultured cell nuclei of fish. Int. J. Biol. Sci. 2010, V. 6, P. 192–198.

      79. Hong N., Li Z., Hong Y. Fish stem cultures. Int. J. Biol. Sci. 2011, 7 (40), 392–402.

      80. Patolsky F., Zheng G. F., Hayden O. Electrical detection of single viruses. Proc. Natl. Acad. Sci. USA. 2004, V. 101, P. 14017–14022.

      81. Li Q. L., Mahendra, S., Lyon D. Y. Antimicrobial nanomaterials for water disinfection and microbial control: potential applications and implications. Water Res. 2008, V. 42, P. 4591–4602.

      82. Pradeep T., Anshup C. Noble metal nanoparticles for water purification: a critical review. Thin Solid Films. 2009, V. 517, P. 6441–6478.

      83. Rud Yu. P., Prilutska S. V., Buchatskii L.P. Application of C60-fullerenes for photodynamic inactivation of  mosquitoes irydoviruses. Patent invention. 2012, Bul. N 2, 05.01.2012.

      84. Rud Yu., Buchatsky L., Prilutskyy Yu. Using C60 fullerenes for photodynamic inactivation of mosquito iridescent virus. J. Enz. Inhib. Med. Chem. 2012, 27 (4), 614–617.

      85. Buchatskiy L.P., Starodub N. F., Nogarev A. V. The use of surface plasmon resonance for the rapid diagnosis of retroviral infections fish. Tez. Vseros. konf. «Probl. patol. immunol. okhr. zdor. ryb i dr. hidrobiont.». Borok. 2003, P. 17–18. (In Russian).

      86. Buchatsky L. P., Starodub N. F. Immune sensor based on surface plasmon resonance for express control of fish retroviral infection. Proc. of the 2-nd bilateral conf. «Aquatic and marine animal health». Shepherdstown. 2003, P. 15.

      87. Rubinsky B., Arav A., Fletcher G. L. Hypothermic protection: a fundamental property of antifreeze proteins. Biochem. Biophys. Res. Commun. 1991, V. 180, 566–571.

      88. Tablin F., Oliver A. E., Walker N. J. Membrane phase transition of intact human platelets: correlation with cold-induced activation. J. Cell Physiol. 1996, V. 168, P. 305–313.<305::AID-JCP9>3.0.CO;2-T

      89. Low W. K., Miao M., Ewart K. V. Skin-type antifreeze protein from the shorthorn sculpin, Myoxocephalus scorpius: expression and characterization of 9,700 recombinant protein. J. Biol. Chem. 1998, V. 273, P. 23098–23103.

      90. Hays L. M., Feeney R. E., Crowe L. M. Antifreeze glycoproteins inhibit leakage from liposomes during thermotropic  phase transitions. Proc. Natl. Acad. Sci. USA. 1996, V. 93, P. 6835–6840.