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Home Archive 2013 № 5 PROSPECTS FOR APPLICATION OF Aplysinidae FAMILY MARINE SPONGE SKELETONS AND MESENCHYMAL STROMAL CELLS IN TISSUE ENGINEERING О. Yu. Rogulska, О. B. Revenko, Yu. O. Petrenko, H. Ehrlich, О. Yu. Petrenko
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ISSN 2410-776X (Online),
ISSN 2410-7751 (Print)

Biotechnologia Acta
V. 6, No 5, 2013



"Biotechnologia Acta" v. 6, no. 5, 2013
doi: 10.15407/biotech6.05.115
Р.115-121, Bibliography 25, Russian
Universal Decimal classification: 591.471+611.018.2/6:57.086.8

PROSPECTS FOR APPLICATION OF Aplysinidae FAMILY MARINE SPONGE SKELETONS AND MESENCHYMAL STROMAL CELLS IN TISSUE ENGINEERING

О. Yu. Rogulska1, О. B. Revenko1, Yu. O. Petrenko1, H. Ehrlich2, О. Yu. Petrenko1

1Institute for problems of Cryobiology and Cryomedicine of National Academy of Sciences of Ukraine, Kharkiv, Ukraine
2Institute of Experimental Physics, TU Bergakademie Freiberg, Freiberg, Germany

Development of the new types of tissue engineered structures is one of the promising trends of current biotechnology. The study was directed to the assessment of prospects for the application of chitin-based skeletons derived from marine sponges of Aplysinidae family (Aplysina fulva and Aplysina aerophoba) for creation of bioengineered constructs based on human mesenchymal stromal cells. After cleaning and demineralization procedures, sponge skeletons appeared as three-dimensional macroporous matrices formed by intersecting chitin fibrils. After seeding into chitin-based matrices the cells were attached to the surface of the fibrils and were able to spread and proliferate. Mesenchymal stromal cells within Aplysina fulva differentiated into osteogenic and adipogenic directions under the influence of appropriate inductors. Demineralized skeletons derived from marine sponges of Aplysinidae family could be used as scaffolds for mesenchymal stromal cells which provides new opportunities for the creation of adipose and bone tissue engineered structures.

Key words: marine sponges, chitin, scaffold, mesenchymal stromal cells, tissue engineering.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008

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Home Archive 2013 № 5 PROSPECTS FOR APPLICATION OF Aplysinidae FAMILY MARINE SPONGE SKELETONS AND MESENCHYMAL STROMAL CELLS IN TISSUE ENGINEERING О. Yu. Rogulska, О. B. Revenko, Yu. O. Petrenko, H. Ehrlich, О. Yu. Petrenko

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