Biotechnologia Acta

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Home Archive 2013 № 4 RECOMBINANT LENTIVIRUS-MEDIATED SILENCING OF ADAPTOR PROTEIN RUK/CIN85 EXPRESSION INFLUENCES BIOLOGICAL RESPONSES OF TUMOR CELLS A. A. Samoylenko, N. V. Byts, G. V. Pasichnyk, N. V. Kozlova, A. V. Bazalii, D. S. Gerashchenko, S. G. Shandrenko, A. V. Vorotnikov, T. Kietzmann, S. V. Komisarenko, L. B. Drobot
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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

Biotechnologia Acta
V. 6, No 4, 2013


"Biotechnologia Acta" v. 6, no. 4, 2013
doi: 10.15407/biotech6.04.182
Р. 182-189, Bibliography 15, Ukrainian
Universal Decimal classification: 577.218

RECOMBINANT LENTIVIRUS-MEDIATED SILENCING OF ADAPTOR PROTEIN RUK/CIN85 EXPRESSION INFLUENCES BIOLOGICAL RESPONSES OF TUMOR CELLS

A. A. Samoylenko1, N. V. Byts1, G. V. Pasichnyk1, N. V. Kozlova1, A. V. Bazalii1, D. S. Gerashchenko1, S. G. Shandrenko1, A. V. Vorotnikov2, T. Kietzmann3, S. V. Komisarenko1, L. B. Drobot1

1Palladian Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv, Ukraine
2Faculty of Fundamental Medicine, Lomonosov Moscow State University, Moscow, Russia
3Department of Biochemistry and Biocenter Oulu, University of Oulu, Oulu, Finland

Ruk/CIN85 is an adaptor protein that plays important roles in the regulation of cellular processes such as cell death, proliferation and motility. It was recently shown that overexpression of Ruk/CIN85 increases the oncogenic potential of human breast adenocarcinoma MCF-7 cells. It was the aim of the present study to investigate whether inhibition of Ruk/CIN85 expression has an effect on the biological properties of the cells. In order to down-regulate Ruk/CIN85 expression of small interfering RNA-based approach was used. For down-regulation of Ruk/CIN85 lentiviral constructs encoding Ruk/CIN85-specific small hairpin RNA sequences were generated. By using the obtained recombinant lentiviruses it was shown that inhibition of Ruk/CIN85 expression influences biological properties (motility, proliferation, ABCG2 expression, and ROS generation) of various tumour cell types such as human breast adenocarcinoma MCF-7, human colorectal adenocarcinoma HT-29, and Lewis mouse lung carcinoma cells.

Key words: adapter proteins, Ruk/CIN85, RNAinterference, lentiviral vectors, cell motility, active oxygen forms.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008

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Home Archive 2013 № 4 RECOMBINANT LENTIVIRUS-MEDIATED SILENCING OF ADAPTOR PROTEIN RUK/CIN85 EXPRESSION INFLUENCES BIOLOGICAL RESPONSES OF TUMOR CELLS A. A. Samoylenko, N. V. Byts, G. V. Pasichnyk, N. V. Kozlova, A. V. Bazalii, D. S. Gerashchenko, S. G. Shandrenko, A. V. Vorotnikov, T. Kietzmann, S. V. Komisarenko, L. B. Drobot

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