Biotechnologia Acta


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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

Biotechnologia Acta
V. 6, No 3, 2013

"Biotechnologia Acta" v. 6, no. 3, 2013
doi: 10.15407/biotech6.03.089
Р. 89-94, Bibliography 17, Ukrainian.
Universal Decimal classification: 582.28:577.158


T. E. Voloshko, О. V. Fedotov

Donetsk National University, Ukraine

The dynamics of growth and catalase activity of 57 strains of basidiomycetes were investigated. Glucose-peptone medium was used for surface cultivation of fungi. The objects of study were 57 strains, 5 of which belongs to 5 species of the order Polyporales, and others do to 7 species of the order Agaricales. The weight measurement to estimate accumulation of absolutely dry biomass was used to study growth rates. The spectrophotometric methods were used for determination of catalase activity and protein content in mycelium and culture filtrate. The specific catalase activity was calculated based on this data. The levels of biomass accumulation and catalase activity of the strains on the 9-th and 12-th days of cultivation and ability of the most fungi to synthesize mainly extracellular catalase were determined. Individual variability of the strains was shown. The results allowed selecting the strains — active producers of catalase, including F. velutipes F-2 and P. ostreatus R-208, which are perspective for use in biotechnology of enzyme preparations.

Key words: basidiomycetes, catalase activity.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008

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