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Home Archive 2013 № 3 ACTIVITY DEPENDENCE OF CELLULOLYTIC ENZYMES MONOAND DIKARYOTIC CULTURES OF Stereum hirsutum (Wild.) Gray (Basidiomycetes) FROM A SUBSTRATUM S. M. Boiko, M. E. Ryazanova
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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

Biotechnologia Acta
V. 6, No 3, 2013

"Biotechnologia Acta" v. 6, no. 3, 2013
doi: 10.15407/biotech6.03.116
Р. 116-120, Bibliography 19, Ukrainian.
Universal Decimal classification: 577.152.321

DIKARYOTIC CULTURES OF Stereum hirsutum (Wild.) Gray (Basidiomycetes) FROM A SUBSTRATUM

S. M. Boiko, M. E. Ryazanova

Donetsk national university, Ukraine

Activity of cellulolytic enzymes cultures Stereum hirsutum (Wild.) Gray, different nuclear status, depending on the substrate have been studied. The most productive by synthesis endoglucanases monokaryotic cultures of Sh1-4 and Sh7-2 were determined. It is found that nutrient medium containing filtering paper is optimal for synthesis of cellulase (culture Sh1-4, maximum of cellulolytic activity is 319 ?М/mg, culture Sh7-2 — 101 ?М/mg). Maximum of cellulolytic activity at cultivation with sawdust of trees for culture Sh1-4 observed at the 10th day was 142 ?М/mg (oak sawdust), for culture Sh7-2 at the 20th days it was 34 ?М/mg (ash-tree sawdust). Study of quality cellulolytic enzymes enabled to establishe presence of four exoenzymes of Cx complex with a relative electrophoretic mobility of 0.22; 0.24; 0.47; 0.74. Enzyme with relative electrophoretic mobility 0.47 showed high concentration and activity.

Key words: cellulolytic enzymes, Stereum hirsutum, monokaryotic cultures, electrophoresis of proteins.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008

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Home Archive 2013 № 3 ACTIVITY DEPENDENCE OF CELLULOLYTIC ENZYMES MONOAND DIKARYOTIC CULTURES OF Stereum hirsutum (Wild.) Gray (Basidiomycetes) FROM A SUBSTRATUM S. M. Boiko, M. E. Ryazanova

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