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Home Archive 2012 № 4 CULTIVATION OF BASIDIOMYCETES WHICH ARE ACTIVE PRODUCERS OF CELLULOLYTIC ENZYMES. ІV. CELLOBIASE ACTIVITY OF BASIDIOMYCETES CULTURAL FILTRATES К. G. Dreval, M. I. Boyko
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ISSN 1995-5537

Scientific journal

БІОТЕХНОЛОГІЯ
v. 5, No. 3, 2012


«Біотехнологія» journal V. 5, No. 4, 2012
Р. 82-87, Bibliography 14, Ukrainian.
Universal Decimal classification: 577.152.321+663.11

CULTIVATION OF BASIDIOMYCETES, WHICH ARE ACTIVE PRODUCERS OF CELLULOLYTIC ENZYMES.
ІV. CELLOBIASE ACTIVITY OF BASIDIOMYCETES CULTURAL
FILTRATES

К. G. Dreval, M. I. Boyko

Donetsk National University

Selection of the cultivation conditions of basidiomycetes, active producers of cellulolytic enzymes, was done along nutrient medium initial acidity (factor was changed between 3 and 9 pH with step 1 pH) and cultivation temperature (factor was changed between 24 and 36 °C with step 2 °C) for the purpose of increasing the synthesis of their cellobiоse (EC 3.2.1.21) production. In the selection process enzymatic activity was determined using cellobiоse as a substrate. It was determined, that optimal initial acidity of the nutrient medium for strains Д-1, А-Дон-02 Irpex lacteus was рН 3, for strain Sh-1 Stereum hirsutum — рН 5 and for strains К-1 Irpex lacteus and AnSc-1 Daedaleopsis confragosa f. confragosa — рН 6; optimal cultivation temperature was 26 °С for strain К-1 Irpex lacteus, 28 °С for strain Д-1 Irpex lacteus, 30 °С for strain AnSc-1 Daedaleopsis confragosa f. confragosa and 32 °С for strains А-Дон-02 Irpex lacteus and Sh-1 Stereum hirsutum. As a result of optimization the values of cellobiase activity increased in 1,11 times for strain А-Дон-02 Irpex lacteus and in 3,82 times for strain К-1 Irpex lacteus. At the same time, as a result of selection of cultivation conditions specific cellobiase activity of strain Д-1 Irpex lacteus was increased in 1,31 times, А-Дон-02 Irpex lacteus — in 1,78 times, К-1 Irpex lacteus — in 2,67 times and AnSc-1 Daedaleopsis confragosa f. confragosa — in 2,79 times. Culture J-2An Phellinus pomaceus showed no cellobiase activity in any experiment. Maximal cellobiase activity of enzymes in cultural liquids of strains Д-1 Irpex lacteus and Sh-1 Stereum hirsutum was established on the 7th day of cultivation, and for strains К-1, А-Дон-02 Irpex lacteus and AnSc-1 Daedaleopsis confragosa f. confragosa — on the 14th day of the experiment.

Key words: basidiomycetes, cellobiase, cultivation temperature, acidity of nutrient medium, optimization, Irpex lacteus, Stereum hirsutum, Daedaleopsis confragosa f. confragosa, Phellinus pomaceus.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008

 

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Home Archive 2012 № 4 CULTIVATION OF BASIDIOMYCETES WHICH ARE ACTIVE PRODUCERS OF CELLULOLYTIC ENZYMES. ІV. CELLOBIASE ACTIVITY OF BASIDIOMYCETES CULTURAL FILTRATES К. G. Dreval, M. I. Boyko

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