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Home Archive 2012 № 3 INDUCTION OF si/miRNA BIOSYNTHESIS WITH ANTIPATHOGENIC AND ANTIPARASITIC PROPERTIES BY GROWTH REGULATORS IN PLANT CELLS V. А. Tsygankova, T. R. Stefanovska, Ya. V. Andrusevich, S. P. Ponomarenko, A. P. Galkin, Ya. B. Blume
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ISSN 1995-5537

Scientific journal

"БІОТЕХНОЛОГІЯ"
V. 5, No. 2, 2012



"Biotechnology" journal V. 5, No. 3, 2012
Р. 62-74, Bibliography 42, Ukrainian.
Universal Decimal classification: 631.811.98 75.117.2.577.2.08

INDUCTION OF si/miRNA BIOSYNTHESIS WITH ANTIPATHOGENIC
AND ANTIPARASITIC PROPERTIES BY GROWTH REGULATORS
IN PLANT CELLS

V. А. Tsygankova, T. R. Stefanovska, Ya. V. Andrusevich,
S. P. Ponomarenko, A. P. Galkin, Ya. B. Blume

Institute of Bioorganic Chemistry and Petrochemistry of National Academy of Sciences of Ukraine, Kyiv
National University of Life and Environmental Science of Ukraine, Kyiv
National Enterprise Interdepartmental Science & Technology Center «Agrobiotech» of National Academy of Sciences and MES of Ukraine, Kyiv
Institute of Food Biotechnology and Genomics of National Academy of Sciences of Ukraine, Kyiv

It was shown in laboratory experiments that compositional polycomponential preparations with bioprotective properties Biogen, Stimpo and Regoplant according to indexes of sprouting and speed of sprout growth considerably increase resistance (to 35–70%) of sugar beet plants to parasitic nematode Heterodera shcachtii and root-knot nematode Meloidogyne incognita and spring wheat to pathogenic micromycete Fusarium oxysporum graminearum. It was reveled that maximal reduction (on 80%) of H. schachtii larvae amount in the sugar beet roots takes place at application of growth regulators in the most effective concentrations: Stimpo — 5 ?g/ml, Regoplant — 1 ?g/ml and Biogen — 1 ?g/ml.

Using DOT-blot hybridization of si/miRNA with mRNA and testing inhibitory activity si/miRNA in cell free system protein biosynthesis is set that resistance of sugar beet against parasitic nematodes H. schachtii and M. incognita as well as resistance of spring wheat to pathogenic micromycete Fusarium oxysporum are reached through enhancement of synthesis of small regulatory si/miRNA.

Key words: plant growth, regulators, small regulatory RNA (si/miRNA), plant resistance to wreckers, cell-free system of wheat germ protein-synthesis.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008

 

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Home Archive 2012 № 3 INDUCTION OF si/miRNA BIOSYNTHESIS WITH ANTIPATHOGENIC AND ANTIPARASITIC PROPERTIES BY GROWTH REGULATORS IN PLANT CELLS V. А. Tsygankova, T. R. Stefanovska, Ya. V. Andrusevich, S. P. Ponomarenko, A. P. Galkin, Ya. B. Blume

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© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2008.
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