Biotechnologia Acta

...

  • Increase font size
  • Default font size
  • Decrease font size
Home Archive 2010 № 3 EFFECT OF SILVER NANOPARTICLES ON THE EXPRESSION OF CIRCADIAN GENES AND 6- PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOS-PHATASE-4 IN RAT BRAIN D. O. Minchenko, I. V. Bozhko, T. O. Zinchenko, V. G. Mykhalchenko, О. P. Yavorovsky, O. G.Minchenko
Print

ISSN 1995-5537

Scientific journal

БІОТЕХНОЛОГІЯ

V. 3, No. 3, 2010

"Biotechnology" journal Vol. 3, No. 3, 2010
Р.
42-49, Bibliography 41, Ukrainian.
Universal Decimal classification
: 577.112.7:616

EFFECT OF SILVER NANOPARTICLES ON THE EXPRESSION OF CIRCADIAN GENES AND 6- PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOS-PHATASE-4 IN RAT BRAIN

D. O. Minchenko, I. V. Bozhko, T. O. Zinchenko, V. G. Mykhalchenko,
О. P. Yavorovsky, O. G.Minchenko

Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv;
National O.O. Bogomoletz Medical University, Kyiv

Physiological and biochemical processes in organisms are circadian. Disturbance of regulation of circadian processes leads to developing of different pathology and cancer in particular. The genes of circadian factors Per1, Per2, Clock and BMal1 as well as caseinkinase-1 ? are important regulators which control these processes in

normal and pathological conditions. We have shown that the expression of caseinkinase-1 ?, BMal1, Per1, Per2 and Clock as well as 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-4 (PFKFB-4) and glyceraldehydes-3-phosphate dehydrogenase (GAPDH) genes significantly destroyed in the brain of rats treated

with silver nanoparticles: expression of some genes increased, but other — decreased. It was shown that changes in the expression of circadian genes and caseinkinase-1 ? were observed in a day after treatment of rats with silver nanoparticles but in 3 and 14 days these changes were more expressed. Disturbance of caseinkinase-1 ? and circadian genes expression could destroy the cellular signal pathways in brain and lead to developing of pathological processes. Results of this investigation demonstrated that silver nanoparticles had significant effect on important regulatory mechanisms controlling metabolic processes in brain cells via circadian gene expression. Expression of circadian genes could be possible a sensitive test for detection of silver nanoparticles action on the organisms.

Key words: silver nanoparticles, gene expression, casein kinase-1 ?, Per1, Per2, Clock, BMal1, PFKFB-4, brain, rats.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008

© Институт биохимии им. А. В. Палладина НАН Украины, 2008

 

Additional menu

Site search

Site navigation

Home Archive 2010 № 3 EFFECT OF SILVER NANOPARTICLES ON THE EXPRESSION OF CIRCADIAN GENES AND 6- PHOSPHOFRUCTO-2-KINASE/FRUCTOSE-2,6-BISPHOS-PHATASE-4 IN RAT BRAIN D. O. Minchenko, I. V. Bozhko, T. O. Zinchenko, V. G. Mykhalchenko, О. P. Yavorovsky, O. G.Minchenko

Invitation to cooperation

Dear colleagues, we invite you to publish your articles in our journal.
© Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, 2008.
All rights are reserved. Complete or partial reprint of the journal is possible only with the written permission of the publisher.
E-mail
for information: biotech@biochem.kiev.ua.