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Home Archive 2011 № 2 PURIFICATION OF TICK-BORNE ENCEPHALITIS VIRUS NON-STRUCTURAL PROTEIN NS1 FROM NON-INFECTIOUS MATERIAL Yu. V. Kuzmenko, S. F. Beresten, E. S. Starodubova, A. V. Timofeev, V. L. Karpov
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ISSN 1995-5537

Scientific journal

БІОТЕХНОЛОГІЯ

V. 4, No. 2, 2011

"Biotechnology" journal V. 4, No. 2, 2011
Р. 39-45, Bibliography 17, Russian.
Universal Decimal classification: 615.371:578.833.25].012.6076.9

PURIFICATION OF TICK-BORNE ENCEPHALITIS VIRUS
NON
-STRUCTURAL PROTEIN NS1
FROM NON
-INFECTIOUS MATERIAL

Yu. V. Kuzmenko, S. F. Beresten, E. S. Starodubova, A. V. Timofeev, V. L. Karpov

Engelhardt Institute of Molecular Biology RAS, Moscow, Russia

Using the following combination of the methods for protein separation: gel-filtration, fast performance liquid chromatography and multiple ultrafiltration, we performed purification of non-structural protein NS1 of tick-borne encephalitis virus from culture fluids of eukaryotic cells transfected with plasmid carrying fulllength gene of NS1. Hence we obtained the sample of nonstructural protein NS1 in the native form that is a hexamer. Current method of purification of NS1 protein from culture fluids of transfected eukaryotic cells completely eliminates risks of infection. This high-purity product can be used to create the new test systems for diagnosis of encephalitis.

Key words: tick-borne encephalitis virus, nonstructural protein NS1.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008

 

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Home Archive 2011 № 2 PURIFICATION OF TICK-BORNE ENCEPHALITIS VIRUS NON-STRUCTURAL PROTEIN NS1 FROM NON-INFECTIOUS MATERIAL Yu. V. Kuzmenko, S. F. Beresten, E. S. Starodubova, A. V. Timofeev, V. L. Karpov

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