ISSN 1995-5537
Scientific journal
БІОТЕХНОЛОГІЯ
V. 4, No. 2, 2011
"Biotechnology" journal V. 4, No. 2, 2011
Р. 46-53, Bibliography 53, Ukrainian.
Universal Decimal classification: 575.222.7:581.1
N. A. Matvieieva, O. M. Kishchenko, A. M. Shakhovsky, M. V. Kuchuk
Institute of Cell Biology and Genetic Engineering of National Academy of Sciences of Ukraine, Kiyv
Agrobacterium rhizogenes-mediated method for genetic transformation of duckweed Lemna minor L. (Lemnaceae, Monocotyledons) without acetosyringone treatment was developed. Transgenic duckweed plants with genes of tuberculosis antigens ESAT6 and Ag85B were obtained with frequency 2% by direct regeneration from axillary meristem without hairy root or callus stage. Developed technique enables to scale down the time for transgenic plant obtaning and to create duckweed plants transformed with genes of interest.
Key words: Lemna minor, Agrobacterium rhizo, genes, genetic transformation, genes of tuberculosis antigens ESAT6 and Ag 85B.
© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008
