THE CONTENT OF PHENOLIC COMPOUNDS AND FLAVONOIDS IN Deschampsia antarctica TISSUE CULTURE M. O. Twardovska, I. I. Konvalyuk, K. V. Lystvan, I. O. Andreev, V. A. Kunakh

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IISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

Biotechnologia Acta V. 14, No 2, 2021
Р. 59-66, Bibliography 24 , English
Universal Decimal Classification:[577.13+543.645]:581.143.6
https://doi.org/10.15407/biotech14.02.059

THE CONTENT OF PHENOLIC COMPOUNDS AND FLAVONOIDS IN Deschampsia antarctica TISSUE CULTURE

M. O. Twardovska ¹, I. I. Konvalyuk ¹, K. V. Lystvan ², I. O. Andreev ¹, V. A. Kunakh ¹

¹Institute of Molecular Biology and Genetics of the National Academy of Sciences of Ukraine, Kyiv
²Institute of Cell Biology and Genetic Engineering of the National Academy of Sciences of Ukraine, Kyiv

Aim. The aim of the study was to determine the quantitative and qualitative content of phenolic compounds and flavonoids in Deschampsia antarctica E. Desv. tissue cultures obtained from plants originating from different islands of the maritime Antarctic.

Methods. In vitro tissue culture, Folin-Ciocalteu method, spectrophotometry, HPLC analysis.

Results. The quantitative content of phenolic compounds and flavonoids in D. antarctica tissue cultures obtained from plants of six genotypes (DAR12, DAR13, G/D12-2a, Y66, R30 and L57) was determined. The highest content of phenolic compounds (4.46 and 3.75 mg/g) was found in tissue cultures obtained from root and leaf explants of plant genotype L57. The highest amount of flavonoids (7.17 mg/g) was accumulated in G/D12-2a tissue culture of root origin. The content of the studied biologically active compounds (BACs) did not change with increasing number of subculture generations (from passage 10 to 19). HPLC analysis showed that in D. antarctica tissue cultures, a shift in the biosynthesis of BACs occurred towards the synthesis of more polar metabolites compared to explant donor plants.

Conclusions. It was found that the transition of cells to undifferentiated growth affected the content of BACs, the amount of which decreased 2–5 times simultaneously with a significant change in their profile. This provided a basis for further biochemical studies, as well as for careful selection of tissue culture of D. antarctica to use it as a potential source of BACs.

Key words: plant tissue culture, Deschampsia antarctica E. Desv., phenolic compounds, flavonoids, HPLC analysis.

References

1. Kunakh V. A. Plant biotechnology for human life improvement. Biotekhnolohiya. 2008, 1 (1), 28-39. (In Ukrainian).

2. Espinosa-Leal C. A., Puente-Garza C. A., Garc?a-Lara S. In vitro plant tissue culture: means for production of biological active compounds. Planta. 2018, 248 (1), 1-18. https://doi.org/10.1007/s00425-018-2910-1

3. Kalynyn F. L., Kushnyr H. P., Sarnatskaya V. V. Technology of microclonal plant propagation. Lobov V. P. (Ed.). Kyiv: Naukova dumka. 1992, 228 p. (In Russian).

4. Kunakh V. A. Biotechnology of medicinal plants. Genetic, physiological and biochemical basis. Kyiv: Logos. 2005, 724 p. (In Ukrainian).

5. George E. F. Plant propagation by tissue culture. Part 1: the technology. Edington: Exegetics Ltd. 1993, 574 p.

6. Loyola-Vargas V. M., Ochoa-Alejo N. An introduction to plant tissue culture: advances and perspectives. Plant Cell Culture Protocols. 2018, P. 3-13. https://doi.org/10.1007/978-1-4939-8594-4_1

7. Fischer R., Vasilev N., Twyman R. M., Schillberg S. High-value products from plants: the challenges of process optimization. Curr. Opin. Biotechnol. 2015, V. 32, P. 156-162. https://doi.org/10.1016/j.copbio.2014.12.018

8. Savithramma N., Linga Rao M., Suhrulatha D. Screening of medicinal plants for secondary metabolites. Middle-East J. Sci. Res. 2011, 8 (3), 579-584.

9. Ramakrishna A., Ravishankar G. Influence of abiotic stress signals on secondary metabolites in plants. Plant Signal Behav. 2011, 6 (11), 1720-1731. https://doi.org/10.4161/psb.6.11.17613

10. Cuba-D?az M., Rivera-Mora C., Navarrete E., Klagges M. Advances of native and nonnative Antarctic species to in vitro conservation: improvement of disinfection protocols. Sci. Reports. 2020, V. 10, P. 3845. https://doi.org/10.1038/s41598-020-60533-1

11. Brakenchielm E. R., Cao Y. Suppression of angiogenesis, tumor growth, and wound healing by resveratrol, a natural compound in red wine and grapes. FASEB J. 2001, V. 15, P. 1798-1800. https://doi.org/10.1096/fj.01-0028fje

12. Gidekel M., Weber H., Cabrera G., Gutierres A., Osorio J., Becera J., Podhajcer O., Cafferata E., Sunkel C., Mihovilovic I. Extracts of Deschampsia antarctica Desv. with antineoplastic activity. U. S. Patent 2010/0310686 A1, December 9, 2010.

13. Malvicini M., Gutierrez-Moraga A., Rodriguez M. M., Gomez-Bustillo S., Salazar L., Sunkel C., Nozal L., Salgado A., Hidalgo M., Lopez-Casas P. P., Novella J. L., Vaquero J.J., Alvarez-Builla J., Mora A., Gidekel M., Mazzolini G. A tricin derivative from Deschampsia antarctica Desv. inhibits colorectal carcinoma growth and liver metastasis through the induction of a specific immune response. Mol. Cancer. Ther. 2018, 17 (5), 966-976. https://doi.org/10.1158/1535-7163.MCT-17-0193

14. Twardovska M. O., Konvalyuk I. I., Lystvan K. V., Andreev I. O., Kunakh V. A. The content of phenolic compounds and flavonoids in in vitro plants and tissue culture of Deschampsia antarctica E. Desv. Factors in Experimental Evolution of Organisms. 2020, V. 26, P. 276-281. https://doi.org/10.7124/FEEO.v26.1279

15. Gamborg O. L., Eveleigh D. E. Culture methods and detection of glucanases in cultures of wheat and barley. Can. J. Biochem. 1968, 46 (5), 417-421. https://doi.org/10.1139/o68-063

16. Konvalyuk I. I., Mozhylevs'ka L. P., Kunakh V. A. Callus initiation and organogenesis in vitro in Deschampsia antarctica E. Desv. Visn. Ukr. tov-va genetikiv i selekcioneriv. 2019, 17 (1), 8-15. (In Ukrainian). https://doi.org/10.7124/visnyk.utgis.17.1.1196

17. Murashige T., Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 1962, 15 (13), 473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

18. Singleton V. L., Rossi J. A. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Vitic. 1965, V. 16, P. 144-158.

19. P?kal A., Pyrzynska K. Evaluation of aluminium complexation reaction for flavonoid content assay. Food Anal. Methods. 2014, 7 (9), 1776-1782. https://doi.org/10.1007/s12161-014-9814-x

20. Twardovska M., Konvalyuk I., Lystvan K., Andreev I., Parnikoza I., Kunakh V. Phenolic and flavonoid contents in Deschampsia antarctica plants growing in nature and cultured in vitro. Polish Polar Res. 2021, in press.

21. Parast B. M., Chetri S. K., Sharma K., Agrawal V. In vitro isolation, elicitation of psoralen in callus cultures of Psoralea corylifolia and cloning of psoralen synthase gene. Plant Physiol. Biochem. 2011, 49 (10), 1138-1146. https://doi.org/10.1016/j.plaphy.2011.03.017

22. Kadkade P. Growth and podophyllotoxin production in callus tissues of Podophyllum peltatum. Plant Sci. Lett. 1982, 25 (1), 107-115. https://doi.org/10.1016/0304-4211(82)90213-9

23. Matkowski A. In vitro isoflavonoid production in callus from different organs of Pueraria lobata (Wild.) Ohwi. J. Plant Physiol. 2004, 161 (3), 343-346. https://doi.org/10.1078/0176-1617-01145

24. Yurin V. M., Dytchenko T. Y., Fylyppova S. N., Molchan O. V., Lohvyna A. O., Hlushakova D. Yu., Spyrydovych Ye. V., Reshetnykov V. N. Collections of callus cultures of medicinal plants. Proceedings of the International Scientific Conference Dedicated to the 85th Anniversary of the Central Botanical Garden of the National Academy of Sciences of Belarus. Minsk. 2017, P. 481-486. (In Russian).

25. Evans D. E., Coleman J. O. D., Kearns A. Plant cell culture. Bios Scientific Publishers, Taylor & Francis Group, London. 2003, 208 p.

26. Eibl R., Meier Ph., Stutz I., Schildberger D., H?hn T., Eibl D. Plant cell culture technology in the cosmetics and food industries: current state and future trends. Appl. Microbiol. Biotechnol. 2018, V. 12, P. 8661-8675. https://doi.org/10.1007/s00253-018-9279-8

27. Nosov A. M. Application of cell technologies for production of plant-derived bioactive substances of plant origin. Appl. Biochem. Microbiol. 2012, V. 48, P. 609-624. https://doi.org/10.1134/S000368381107009X

28. Reinert J., Yeoman M. M. The production of the steroid, diosgenin, from tissue cultures of Dioscorea deltoidea. Plant Cell and Tissue Culture. 1982, Р. 51-54. https://doi.org/10.1007/978-3-642-81784-7_15

29. Kunakh V. A. Evolution of cell populations in vitro: peculiarities, driving forces, mechanisms and consequences. Biopolym. Cell. 2013, 29 (4), 295-310. https://doi.org/10.7124/bc.000824

30. Lystvan K., Kumorkiewicz A., Szneler E., Wybraniec S. Study on betalains in Celosia cristata Linn. callus culture and identification of new malonylated amaranthins. J. Agricult. Food Сhem. 2018, 66 (15), 3870-3879. https://doi.org/10.1021/acs.jafc.8b01014

31. Cuba M., Guti?rrez-Moraga A., Butendieck B., Gidekel M. Micropropagation of Deschampsia antarctica - a frost-resistant Antarctic plant. Antarctic Sci. 2005, 17 (1), 69-70. https://doi.org/10.1017/S0954102005002440

32. Navarro G. E. Z., Honorato A. E. S., Oyarzun A. G. C., Rodrigues E. A. T., Encalada H. G. P., Cantillana P. A. Z. Thermo-photo-bioreactor and method for the culture and mass micropropagation of Deschampsia аntarctica in vitro. U. S. Patent 2013/0344528 A1, December 26, 2013.

33. Sequeida A., Tapia E., Ortega M. Production of phenolic metabolites by Deschampsia antarctica shoots using UV-B treatments during cultivation in a photobioreactor. Electronic J. Biotechnol. 2012, 15 (4), 1-8. https://doi.org/10.2225/vol15-issue4-fulltext-7

34. Twardovska M. O., Drobyk N. M., Mel?nyk V. M., Konvalyuk I. I., Kunakh V. A. Genome variability of some Gentiana L. species in nature and in culture in vitro: RAPD-analysis. Biopolym. Cell. 2010. 26 (6), 499-507. https://doi.org/10.7124/bc.00017A