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Home Archive 2009 № 2 OPTIMIZING PROTEIN A-COLLOIDAL GOLD CONJUGATES SYNTHESIS CONDITIONS AND DEVELOPING APPROACHES FOR THEIR CHARACTERIZATION A. Yu. Labyntsev, O. S. Oliinyk, A. A. Kaberniuk, O. Yu. Chunihin V. F. Gorchev, T. O. Kyrchenko, V. I. Chernushov, D. V. Kolibo
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ISSN 1995-5537

Scientific journal

БІОТЕХНОЛОГІЯ

V. 2, No. 2, 2009


"Biotechnology" journal Vol. 2, No. 2, 2009
Р. 78-85, Bibliography 13, Ukrainian.
Universal Decimal classification: 577(087.1+112.4)


Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv

Approach for deriving conjugates of gold nanoparticles with Staphylococcus aureus protein A recombinant fragment for immunochromatograpical test-systems was optimized. Influence of main components in reaction solution on particle size of gold and its conjugates with protein A fragment, their homogeneity, stability and immunoglobulin-recognition ability was investigated.

Methods for rapid size determination of gold nanoparticles based on their optical characteristics were proposed. Recommendations for deriving staphylococcal protein A fragment-gold nanoparticle conjugates that could be successfully used in fast immunochromatograpical test-systems were given.

Key words: gold nanoparticles, protein А, Staphylococcus aureus, immunoglobulinrecognition ability, immunochromatograpical test-systems, electron microscopy, laser correlation spectroscopy, spectrophotometry, RGB spectrum.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2008

 

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Home Archive 2009 № 2 OPTIMIZING PROTEIN A-COLLOIDAL GOLD CONJUGATES SYNTHESIS CONDITIONS AND DEVELOPING APPROACHES FOR THEIR CHARACTERIZATION A. Yu. Labyntsev, O. S. Oliinyk, A. A. Kaberniuk, O. Yu. Chunihin V. F. Gorchev, T. O. Kyrchenko, V. I. Chernushov, D. V. Kolibo

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