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Home Archive 2022 № 3 Chelidonium majus WATER EXTRACT INITIATES PLATELET AGGREGATION AND INHIBITS FIBRIN POLYMERIZATION IN BLOOD PLASMA R. Yu. Marunych, V. O. Chernyshenko, T. M. Chernyshenko, S. O. Kalashnyk, V. O. Menshova, E. M. Makogonenko, A.V. Gudzenko
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ISSN 2410-7751 (Print)
ISSN 2410-776X (Online)

Biotechnologia Acta  V. 15, No. 3, 2022
P. 29-34. Bibliography 16, Engl.
UDC: 577.11; 601; 615.32; 616
https://doi.org/10.15407/biotech15.03.029

Chelidonium majus WATER EXTRACT INITIATES PLATELET AGGREGATION AND INHIBITS FIBRIN POLYMERIZATION IN BLOOD PLASMA

R. Yu. Marunych 1, V. O. Chernyshenko 1, T. M. Chernyshenko 1, S. O. Kalashnyk 2,
V. O. Menshova 2, E. M. Makogonenko 1, A.V. Gudzenko
3

1 Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, Kyiv
2 Fomin Botanical garden, NSC "Institute of Biology and Medicine"of Taras Shevchenko National University of Kyiv, Ukraine
3 PHEE “Kyiv Medical University”, Ukraine

Chelidonium majus is a well-known source of biologically active compounds. Most of them are alkaloids, which are used in researches and for tradition medicine. In this study, we explored the influence of C. majus crude total extract onto blood coagulation system in vitro, primary and secondary hemostasis.

Aim. To study influence of C. majus extract onto blood coagulation process.

Methods. Turbidimetry of blood plasma, APTT test with chromogenic substrates, and platelet aggregation were used in the work.

Results. We demonstrated moderate stimulating effect of the extract on platelets (the rate of platelet aggregation increased up to 10%, followed by disaggregation). Extract also increased the rate of platelet aggregation stimulated by 12.5 mcM of ADP.

We observed the increase in the plasma clotting time in the presence of the extract, that corresponded to the 274, 411, 685, 1370 mcg of dry extract, from 70 s in control to 80, 90, 170 and 180 s, respectively, in turbidimetry test of plasma stimulated by APTT-reagent.

However, APTT test with the chromogenic substrate of thrombin (S2238) did not show significant influence of this plant’s extract on thrombin activity.

Conclusions. Thus, we can conclude that anticoagulant activity of Chelidonium majus extract corresponded to the direct inhibition of fibrin polymerization.

Key words: Chelidonium majus, platelet activation, plasma coagulation, thrombin.

© Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, 2022

  • References
    • 1. Aronson J. K., Meyler L. Desai C. Meyler's side effects of drugs: The international encyclopedia of adverse drug reactions and interactions. Amsterdam : Elsevier Science, 2016. Available at https://www.sciencedirect.com/referencework/9780444537164/meylers-side-effects-of-drugs (Acessed, July, 2022)

      2. Potopalskij A. I. Celandine preparations in biology and medicine. Kiev, Naukova dumka, 1992, 1-239, Russian.

      3. T?borsk? E, Bochor?kov? H, Paulov? H, Dost?l J. Separation of Alkaloids in Chelidonium majus by Reversed Phase HPLC. Planta Med. 1994, 60(4), 380?381. https://doi.org/10.1055/s-2006-959508

      4. Barabanov E. I., Zaichikova S. G. Botany: a textbook for higher educational institutions. Moscow, Publishing Center “Academy”, 2006, 1-448. (In Russian).

      5. Zieli?ska S, W?jciak-Kosior M, Dzi?gwa-Becker M, Gle?sk M, Sowa I, Fija?kowski K, Rura?ska-Smutnicka D, Matkowski A, Junka A. The Activity of Isoquinoline Alkaloids and Extracts from Chelidonium majus against Pathogenic Bacteria and Candida sp. Toxins (Basel). 2019, 11(7), 406. https://doi.org/10.3390/toxins11070406

      6. Nawrot R, Warowicka A, Rudzki P.J, Musidlak O, Dolata K.M, Musijowski J, Stolarczyk E.U, Go?dzicka-J?zefiak A. Combined Protein and Alkaloid Research of Chelidonium majus Latex Reveals CmMLP1 Accompanied by Alkaloids with Cytotoxic Potential to Human Cervical Carcinoma Cells. Int J Mol Sci. 2021, 22(21), 11838. https://doi.org/10.3390/ijms222111838

      7. Och A, Zalewski D, Komsta ?, Ko?odziej P, Kocki J, Bogucka-Kocka A. Cytotoxic and Proapoptotic Activity of Sanguinarine, Berberine, and Extracts of Chelidonium majus L. and Berberis thunbergii DC. toward Hematopoietic Cancer Cell Lines. Toxins (Basel). 2019, 11(9), 485. https://doi.org/10.3390/toxins11090485

      8. Gracz-Bernaciak J., Mazur O., Nawrot R. Functional Studies of Plant Latex as a Rich Source of Bioactive Compounds: Focus on Proteins and Alkaloids. Int J Mol Sci. 2021, 22(22), 12427. https://doi.org/10.3390/ijms222212427

      9. Nawrot R. Defense-related Proteins from Chelidonium majus L. as Important Components of its Latex. Curr Protein Pept Sci. 2017, 18(8), 864?880. https://doi.org/10.2174/1389203718666170406124013

      10. Xia L. M., Luo M. H. Study progress of berberine for treating cardiovascular disease. Chronic Dis Transl Med. 2016, 1(4), 231?235. https://doi.org/10.1016/j.cdtm.2015.11.006

      11. Anti-platelet Effect of Berberine in Patients After Elective Percutaneous Coronary Intervention (APLABE-PCI). ClinicalTrials.gov Id: NCT03378934. 2017?2018;

      12. Deulenko V. N., Marunych R. Y., Chernyshenko V. O., Rebriev A. V.,  Lugovskoy E. V., Garmanchuck L. V., Pavliuk O. V. Influence of Chelidonium majus Water Extract onto Cancer Cells and Platelets. Biochem Anal Biochem. 2018; 7(4), 370. https://doi.org/10.4172/2161-1009.1000370

      13. Paul M., Hemshekhar M., Kemparaju K., Girish K. S. Berberine mitigates high glucose-potentiated platelet aggregation and apoptosis by modulating aldose reductase and NADPH oxidase activity. Free Radic Biol Med. 2019, 130, 196?205. https://doi.org/10.1016/j.freeradbiomed.2018.10.453

      14. Horak I., Prylutska S., Krysiuk I., Luhovskyi S., Hrabovsky O., Tverdokhleb N., Franskevych D., Rumiantsev D., Senenko A., Evstigneev M., Drobot L., Matyshevska O., Ritter U., Piosik J., Prylutskyy Y. Nanocomplex of Berberine with C60 Fullerene Is a Potent Suppressor of Lewis Lung Carcinoma Cells Invasion In Vitro and Metastatic Activity In Vivo. Materials (Basel). 2021, 14(20), 6114. https://doi.org/10.3390/ma14206114

      15. Xiao C. W, Liu Y., Wei Q., Ji Q. A., Li K., Pan L. J., Bao G. L. Inhibitory Effects of Berberine Hydrochloride on Trichophyton mentagrophytes and the Underlying Mechanisms. Molecules. 2019, 24(4):742. https://doi.org/10.3390/molecules24040742

      16. Caymanchem.com, chemical products, cat. Number 10006427. Available at https://cdn.caymanchem.com/cdn/insert/10006427.pdf  (Acessed, July, 2022).


 

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Home Archive 2022 № 3 Chelidonium majus WATER EXTRACT INITIATES PLATELET AGGREGATION AND INHIBITS FIBRIN POLYMERIZATION IN BLOOD PLASMA R. Yu. Marunych, V. O. Chernyshenko, T. M. Chernyshenko, S. O. Kalashnyk, V. O. Menshova, E. M. Makogonenko, A.V. Gudzenko

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